Dataset on the diversity of plant-parasitic nematodes in cultivated olive trees in southern Spain

Incidence and nematode population densities of plant-parasitic nematodes were determined in 64 samples of soil and grapevine roots collected from commercial vineyards in southern Spain between October 2003 and May 2005. In addition, a histopathological study was done of root-stock roots naturally infected by root-knot nematodes (Meloidogyne spp.). Nematodes infecting the rootstocks were identified according to conventional procedures, and the Meloidogyne spp. were furthermore identified by sequence characterized amplified region-polymerase chain reaction (SCAR-PCR) and isozyme esterase analyses. The most important plant-parasitic nematodes detected, in order of decreasing frequency of total soil infestation and root infection (percentage of samples), were Mesocriconema xenoplax (34.4%), Meloidogyne incognita (26.6%), Meloidogyne javanica (14.1%), Xiphinema index (12.5%), Xiphinema italiae (10.9%), Pratylenchus vulnus (6.3%), and Meloidogyne arenaria (1.6%). No disease symptoms were observed on aboveground plant parts of the infected grapevines, except for plants in some fields where soil was infested with the virus-vector nematodes X. index and X. italiae. Those grapevines showed a yellow mosaic pattern in leaves early in the growing season and the internode shortening characteristic of infections by Grapevine fanleaf virus. Rootstocks infected by root-knot nematodes (Meloidogyne spp.) showed distorted feeder roots and large- to moderate-sized root galls, present either singly or in clusters. Histopathology of galled roots showed a typical susceptible response to infection by root-knot nematodes: cellular alterations were induced in the cortex, endodermis, pericycle, and vascular system, including giant-cell formation and severe distortion of vascular tissues. Most Meloidogyne egg masses ocurred on the surface of the galled root tissues, a position that could facilitate dispersion of the nematode eggs and juveniles and the occurrence of secondary infections. Some of the grapevine rootstocks surveyed in this study (Paulsen 1103, Richter 110, Rupestris du Lot, and SO4) had previously been reported to be resistant to Meloidogyne spp.; however, the population densities of these nematodes found in soil and roots sampled in the present study, as well as the compatible host-parasite relationship revealed by histopathology, indicate a susceptible response to Meloidogyne spp. from southern Spain.

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Incidence of plant-parasitic nematodes in natural and semi-natural mountain grassland and the host status of some common grass species

Nematology ◽

10.1163/156854102760290518 ◽

2002 ◽

Vol 4 (4) ◽

pp. 541-552 ◽

Cited By ~ 11

Author(s):

Alfonso Navas ◽

Miguel Talavera

Keyword(s):

Parasitic Nematode ◽

Southern Spain ◽

Grass Species ◽

Parasitic Nematodes ◽

Plant Parasitic Nematodes ◽

Forage Legumes ◽

Pasture Legumes ◽

Mountain Grassland ◽

Host Status ◽

Plant Parasitic

AbstractA plant-parasitic nematode survey was undertaken in mountainous pastures of southern Spain. Sixty-three species of plant-parasitic nematodes distributed over 25 genera were associated with pastures and grasslands in southern Spain. Paratylenchus species (P. microdorus, P. similis, P. nanus and P. ciccaronei) were the most abundant and prevalent plant-parasitic nematodes found. Pratylenchus neglectus and P.thornei were widely distributed in 55 and 41% of sites, respectively. Amplimerlinius globigerus, Helicotylenchus digonicus, H. dihystera, H. tunisiensis, Merlinius brevidens, M. microdorus, Rotylenchus unisexus and Scutylenchus quadrifer were found in more than 10% of the fields, on occasion at densities of more than 200 per 100 cm³ of soil. A further 49 species of plant-parasitic nematodes were identified and reported. A pot experiment, using a range of grasses and forage legumes adapted to the zone, ascertained the host status of the most abundant plant-parasitic nematodes. Helicotylenchus digonicus, M. microdorus and P.microdorus populations were maintained or increased by all grasses or legumes tested. In general, grasses were better hosts of P.neglectus and P.thornei than the pasture legumes tested. Medicago minima reduced P.thornei densities after 3 months of growing.

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Plant-parasitic nematodes associated with cultivated and wild olive trees in Crete, Greece

Hellenic Plant Protection Journal ◽

10.2478/hppj-2020-0003 ◽

2020 ◽

Vol 13 (1) ◽

pp. 24-28 ◽

Cited By ~ 1

Author(s):

A. Archidona-Yuste ◽

C. Cantalapiedra-Navarrete ◽

J.E. Palomares-Rius ◽

P. Castillo ◽

E.A. Tzortzakakis

Keyword(s):

Nematode Species ◽

Parasitic Nematodes ◽

Plant Parasitic Nematodes ◽

Wild Olive ◽

Olive Trees ◽

First Time ◽

Plant Parasitic

SummaryThe present study is part of a survey for the identification of plant-parasitic nematodes in the rhizosphere of cultivated and wild olive trees in Crete, Greece. Sixteen species corresponding to 13 genera are added to 20 species belonging to 8 genera, previously reported in the survey. Seven nematode species, Filenchus ditissimus, Filenchus vulgaris, Ogma civellae, Pratylenchoides crenicauda, Psilenchus hilarulus, Tylenchus elegans, and Zygotylenchus guevarai, are recorded for the first time in Greece.

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Incidence and Population Density of Plant-Parasitic Nematodes Associated with Olive Planting Stocks at Nurseries in Southern Spain

Plant Disease ◽

10.1094/pdis.2002.86.10.1075 ◽

2002 ◽

Vol 86 (10) ◽

pp. 1075-1079 ◽

Cited By ~ 28

Author(s):

A. I. Nico ◽

H. F. Rapoport ◽

R. M. Jiménez-Díaz ◽

P. Castillo

Keyword(s):

Vascular System ◽

Cell Formation ◽

Southern Spain ◽

Parasitic Nematodes ◽

Plant Parasitic Nematodes ◽

Root Knot Nematode ◽

Population Densities ◽

Histopathological Studies ◽

Root Tissues ◽

Plant Parasitic

Nematode population densities were determined in 259 soil and root samples collected from 18 olive nurseries in Córdoba, Jaén, and Sevilla provinces (southern Spain), between October 1997 and May 1998. The most important plant-parasitic nematodes detected, in order of decreasing frequency of infestation (percentage of samples), were Mesocriconema xenoplax (39.0%), Pratylenchus penetrans (32.1%), P. vulnus (25.9%), Meloidogyne incognita (14.7%), M. javanica (11.2%), and M. arenaria (2.7%). No disease symptoms were noted on aboveground organs of infected plants. However, population densities of Pratylenchus and Meloidogyne spp. were at potentially damaging levels in most of the olive nurseries surveyed. Histopathological studies of galled roots from the naturally infected olive planting stocks showed a susceptible response to root-knot nematode infection. Large numbers of egg masses were present within the galled root tissues that might contribute to secondary infections. Feeding by root-knot nematodes induced the expected cellular alterations in the cortex, endodermis, pericycle, and vascular system, including giant-cell formation and the alteration of vascular tissues.

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Nematode Management in the Strawberry Fields of Southern Spain

Agronomy ◽

10.3390/agronomy9050252 ◽

2019 ◽

Vol 9 (5) ◽

pp. 252 ◽

Cited By ~ 2

Author(s):

Miguel Talavera ◽

Luis Miranda ◽

José Antonio Gómez-Mora ◽

María Dolores Vela ◽

Soledad Verdejo-Lucas

Keyword(s):

Tolerance Limit ◽

Southern Spain ◽

Parasitic Nematodes ◽

Soil Nematode ◽

Pratylenchus Penetrans ◽

Plant Parasitic Nematodes ◽

Population Densities ◽

The Impact ◽

Plant Parasitic ◽

Strawberry Cultivars

(1) Background: Spain is the sixth strawberry producer in the world, with about 6500 ha producing more than 350,000 tons, and an annual commercial value about 390 million €. Stunted and dead strawberry plants are frequently associated with plant-parasitic nematodes, but nematode diseases have not been characterized to date in the country. (2) Methods: A poll on the perception of the impact of nematodes on strawberry production was carried out by face-to-face interviews with farm advisors. In addition, nematological field surveys were carried out at the end of the growing season in 2017 and 2018 to determine prevalence and abundance of plant-parasitic nematodes in strawberry crops. The host suitability to Meloidogyne hapla of seventeen strawberry cultivars and the tolerance limit to M. hapla at progressively higher initial population densities (Pi) were assessed in pot experiments in a growth chamber. Comparison of the relative efficacies of several soil disinfestation methods in controlling nematode populations (M. hapla and Pratylenchus penetrans) was carried out in experimental field trials for twelve consecutive years. (3) Results: Meloidogyne spp., Pratylenchus penetrans, and Hemicycliophora spp. were the main plant-parasitic nematodes in the strawberry fields in South Spain. Root-knot nematodes were found in 90% of the fields, being M. hapla the most prevalent species (71% of the fields). A tolerance limit of 0.2 M. hapla juveniles per g of soil was estimated for strawberry, and currently cropped strawberry cultivars did not show resistance to M. hapla. Nematode population densities were reduced by more than 70% by soil fumigation with 1,3-dichloropropene, dazomet, dimethyl-disulfide, and methyl iodide. The efficacy of metam-sodium in reducing nematode populations was about 50% and that of chloropicrin, furfural, and sodium-azide, less than 40%. Combination of solarization with organic manures (biosolarization) reduced soil nematode populations by 68–73%. (4) Conclusions: Plant-parasitic nematodes (Meloidogyne, Pratylenchus, and Hemicycliophora) are widely distributed in the strawberry fields of Southern Spain. Strawberry is a poor host for M. hapla with a tolerance limit of 0.2 J2 per g of soil, and low population increases in cropping cycles of 7–8 months. Strawberry cultivars show a range of susceptibility and tolerance to M. hapla, but no resistance is found. Nematodes are effectively controlled by chemical fumigation of soils, but soil biosolarization is equally effective, and therefore, can be proposed as a sustainable alternative for pathogen control in strawberry cultivation.

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Plant Parasitic Nematodes Associated With Chickpea in Southern Spain and Effect of Soil Temperature On Reproduction of Pratylenchus Thornei

Nematologica ◽

10.1163/004325996x00057 ◽

1996 ◽

Vol 42 (2) ◽

pp. 211-219 ◽

Cited By ~ 17

Author(s):

A. Gomez-Barcina ◽

P. Castillo ◽

R.M. Jimenez-Diaz

Keyword(s):

Soil Temperature ◽

Southern Spain ◽

Parasitic Nematodes ◽

Plant Parasitic Nematodes ◽

Pratylenchus Thornei ◽

Plant Parasitic

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Can plants with good anthelmintic activity against free-living and animal parasitic nematodes be effective against plant parasitic nematodes?

10.1055/s-0039-3399736 ◽

2019 ◽

Author(s):

FN Makhubu ◽

MC Khosa ◽

LJ McGaw

Keyword(s):

Anthelmintic Activity ◽

Parasitic Nematodes ◽

Plant Parasitic Nematodes ◽

Free Living ◽

Plant Parasitic

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Management of Plant-Parasitic Nematodes in Florida Cotton Production

EDIS ◽

10.32473/edis-ng015-2017 ◽

2017 ◽

Vol 2017 (2) ◽

pp. 8

Author(s):

Zane Grabau

Keyword(s):

Parasitic Nematodes ◽

Cotton Production ◽

Plant Parasitic Nematodes ◽

Fact Sheet ◽

Plant Parasitic

This 8-page fact sheet written by Zane J. Grabau and published in January 2017 by the UF Department of Entomology and Nematology explains how to diagnose and manage nematode problems in cotton production.http://edis.ifas.ufl.edu/ng015

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