Novel near-infrared pH-sensitive cyanine-based fluorescent probes for intracellular pH monitoring

2019 ◽  
Vol 170 ◽  
pp. 107612 ◽  
Author(s):  
Di Jin ◽  
Bowei Wang ◽  
Yuqing Hou ◽  
Yuchao Du ◽  
Xue Li ◽  
...  
Keyword(s):  
Intracellular Ph ◽  
Fluorescent Probes ◽  
Near Infrared ◽  
Ph Monitoring ◽  
Ph Sensitive

Two near-infrared highly sensitive cyanine fluorescent probes for pH monitoring

2017 ◽  
Vol 28 (8) ◽  
pp. 1681-1687 ◽  
Author(s):  
Jing-Ru Hou ◽  
Di Jin ◽  
Bo Chen ◽  
Lei-Lei Si ◽  
Yue-Hua Jin ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Near Infrared ◽  
Ph Monitoring ◽  
Highly Sensitive

Near-infrared fluorescent probes with higher quantum yields and neutral pKa values for the evaluation of intracellular pH

RSC Advances ◽  
2016 ◽  
Vol 6 (73) ◽  
pp. 69641-69646 ◽  
Author(s):  
Xinbo Song ◽  
Mingyu Hu ◽  
Chao Wang ◽  
Yi Xiao
Keyword(s):  
High Resolution ◽  
Intracellular Ph ◽  
Fluorescent Probes ◽  
Near Infrared ◽  
Quantum Yields ◽  
Pka Values

Near-infrared fluorescent probes for pH, named pH-A and pH-B, for labeling cells to produce high resolution fluorescent images reflect the changes of intracellular pH.

LONG TERM EFFECTS OF THYROID STIMULATING HORMONE AND INSULIN ON INTRACELLULAR pH IN FRTL-5 CELLS

1992 ◽  
Vol 133 (2) ◽  
pp. R9-R11
Author(s):  
A.M. Wood ◽  
S.P. Bidey ◽  
J. Soden ◽  
W.R. Robertson
Keyword(s):  
Intracellular Ph ◽  
Small Groups ◽  
Thyroid Stimulating Hormone ◽  
Ph Sensitive ◽  
Long Term Effects ◽  
Bicarbonate Ions ◽  
Chronic Effects ◽  
Petri Dishes ◽  
Presence And Absence

ABSTRACT We have studied the chronic effects of TSH (100μU/ml) and insulin (10μg/ml) on intracellular pH (pHi) in FRTL-5 cells using the pH sensitive probe 2′7-bis (2-carboxyethyl-5′-6′) carboxyfluorescein. FRTL-5 cells were cultured on Petri dishes either in the presence of 4H, ie. Coons F-12 containing cortisol (10nM), transferrin (0.5μg/ml), glycyl-histidyl lysine acetate (10ng/ml) and somatostatin (10μg/ml), or with 4H+insulin (5H), 4H+TSH, or 4H+TSH+insulin (6H). pHi was measured in small groups of cells by microspectrofluorimetry both in the presence and absence of bicarbonate ions after cells had been deprived of serum for at least a day. In

scholarly journals A Near-Infrared Fluorescent Probe Based on a FRET Rhodamine Donor Linked to a Cyanine Acceptor for Sensitive Detection of Intracellular pH Alternations

Molecules ◽  
2018 ◽  
Vol 23 (10) ◽  
pp. 2679 ◽  
Author(s):  
Yibin Zhang ◽  
Jianheng Bi ◽  
Shuai Xia ◽  
Wafa Mazi ◽  
Shulin Wan ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Intracellular Ph ◽  
Near Infrared ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Sensitive Detection ◽  
Solution Ph ◽  
Acidic Conditions ◽  
Ph Level ◽  
Rhodamine Dye

A fluorescence resonance energy transfer (FRET)-based near-infrared fluorescent probe (B+) for double-checked sensitive detection of intracellular pH changes has been synthesized by binding a near-infrared rhodamine donor to a near-infrared cyanine acceptor through robust C-N bonds via a nucleophilic substitution reaction. To demonstrate the double-checked advantages of probe B+, a near-infrared probe (A) was also prepared by modification of a near-infrared rhodamine dye with ethylenediamine to produce a closed spirolactam residue. Under basic conditions, probe B+ shows only weak fluorescence from the cyanine acceptor while probe A displays nonfluorescence due to retention of the closed spirolactam form of the rhodamine moiety. Upon decrease in solution pH level, probe B+ exhibits a gradual fluorescence increase from rhodamine and cyanine constituents at 623 nm and 743 nm respectively, whereas probe A displays fluorescence increase at 623 nm on the rhodamine moiety as acidic conditions leads to the rupture of the probe spirolactam rings. Probes A and B+ have successfully been used to monitor intracellular pH alternations and possess pKa values of 5.15 and 7.80, respectively.

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