In vivo imaging of green fluorescent protein-expressing cells in transgenic animals using fibred confocal fluorescence microscopy

2006 ◽  
Vol 85 (8) ◽  
pp. 837-845 ◽  
Author(s):  
Kaïs H. Al-Gubory ◽  
Louis-Marie Houdebine
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescence Microscopy ◽  
In Vivo Imaging ◽  
Fluorescent Protein ◽  
Transgenic Animals ◽  
Confocal Fluorescence Microscopy ◽  
Confocal Fluorescence ◽  
Green Fluorescent

Intraocular in vivo imaging of activated T-lymphocytes expressing green-fluorescent protein after stimulation with endotoxin

2001 ◽  
Vol 239 (8) ◽  
pp. 609-612 ◽  
Author(s):  
Matthias D. Becker ◽  
Sergio Crespo ◽  
Tammy M. Martin ◽  
Stephen R. Planck ◽  
Mayumi Naramura ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
T Lymphocytes ◽  
In Vivo Imaging ◽  
Fluorescent Protein ◽  
Activated T Lymphocytes ◽  
Green Fluorescent

scholarly journals Three-Dimensional in Vivo Imaging of Green Fluorescent Protein-Expressing T Cells in Mice with Noncontact Fluorescence Molecular Tomography

2007 ◽  
Vol 6 (2) ◽  
pp. 7290.2007.00007 ◽  
Author(s):  
Anikitos Garofalakis ◽  
Giannis Zacharakis ◽  
Heiko Meyer ◽  
Eleftherios N. Economou ◽  
Clio Mamalaki ◽  
...  
Keyword(s):  
T Cells ◽  
Green Fluorescent Protein ◽  
In Vivo Imaging ◽  
Fluorescent Protein ◽  
Three Dimensional ◽  
Fluorescence Molecular Tomography ◽  
Green Fluorescent

scholarly journals Lab-on-a-chip technologies for proteomic analysis from isolated cells

2008 ◽  
Vol 5 (suppl_2) ◽  
Author(s):  
H Sedgwick ◽  
F Caron ◽  
P.B Monaghan ◽  
W Kolch ◽  
J.M Cooper
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescence Microscopy ◽  
Fluorescent Protein ◽  
Single Cells ◽  
Lab On A Chip ◽  
Carcinoma Cells ◽  
Isolated Cells ◽  
Confocal Fluorescence ◽  
On Chip ◽  
Green Fluorescent

Lab-on-a-chip systems offer a versatile environment in which low numbers of cells and molecules can be manipulated, captured, detected and analysed. We describe here a microfluidic device that allows the isolation, electroporation and lysis of single cells. A431 human epithelial carcinoma cells, expressing a green fluorescent protein-labelled actin, were trapped by dielectrophoresis within an integrated lab-on-a-chip device containing saw-tooth microelectrodes. Using these same trapping electrodes, on-chip electroporation was performed, resulting in cell lysis. Protein release was monitored by confocal fluorescence microscopy.

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