In situ electrochemical analysis of alkaline phosphatase activity in 3D cell cultures

2020 ◽  
Vol 359 ◽  
pp. 136951
Author(s):  
Claudia Caviglia ◽  
Rodrigo Pimentel Carletto ◽  
Stefania De Roni ◽  
Yasmin Mohamed Hassan ◽  
Suhith Hemanth ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Cell Cultures ◽  
Alkaline Phosphatase Activity ◽  
Electrochemical Analysis ◽  
3D Cell Cultures

A simple thermometer-based photothermometric assay for alkaline phosphatase activity based on target-induced nanoprobe generation

2020 ◽  
Vol 44 (41) ◽  
pp. 17753-17760
Author(s):  
Honghong Rao ◽  
Huiyi Huang ◽  
Xinyuan Zhang ◽  
Xin Xue ◽  
Mingyue Luo ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Prussian Blue ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Prussian Blue Nanoparticles

Alkaline phosphatase (ALP)-induced in situ generation of Prussian blue nanoparticles for photothermometric ALP detection.

scholarly journals Alkaline inorganic pyrophosphatase activity of mammalian-cell alkaline phosphatase

1967 ◽  
Vol 105 (1) ◽  
pp. 155-161 ◽  
Author(s):  
Rody P. Cox ◽  
Paul Gilbert ◽  
Martin J. Griffin
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Mammalian Cell ◽  
Cell Cultures ◽  
Alkaline Phosphatase Activity ◽  
Inorganic Pyrophosphatase ◽  
Mammalian Cell Cultures ◽  
Single Protein ◽  
Pyrophosphatase Activity ◽  
Substrate Induction

Alkaline phosphatase prepared from mammalian cell cultures was found to have alkaline inorganic pyrophosphatase activity. Both of these activities appear to be associated with a single protein, as demonstrated by: (1) concomitant purification of alkaline phosphatase and alkaline inorganic pyrophosphatase; (2) proportional precipitation of alkaline phosphatase and inorganic pyrophosphatase activities by titrating constant amounts of an enzyme preparation with increasing concentration of antibody; (3) immune electrophoresis, which showed that precipitin bands that have alkaline phosphatase activity also have pyrophosphatase activity; (4) inhibition of pyrophosphatase activity by cysteine, an inhibitor of alkaline phosphatase activity; (5) similar subcellular localization of the two enzyme activities as demonstrated by histochemical methods; (6) hormonal and substrate induction of alkaline phosphatase activity in mammalian cell cultures, which produced a nearly parallel rise in inorganic pyrophosphatase activity.

Influence of Light on the Alkaline Phosphatase Activity of Selenastrum capricornutum (Chlorophyceae) in Streams

1985 ◽  
Vol 42 (2) ◽  
pp. 384-388 ◽  
Author(s):  
R. L. Klotz
Keyword(s):  
New York ◽  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
New York State ◽  
Selenastrum Capricornutum ◽  
Alkaline Phosphatases ◽  
Reactive Phosphorus ◽  
First Time

The alkaline phosphatase activity (APA) of Selenastrum capricomutum Printz incubated in situ in four streams in New York State was inversely related to total insolation. APA was not correlated with stream molybdate reactive phosphorus over the range of concentrations encountered. Selenastrum showed no diel cycle of APA. The phosphorus fraction made available by the activity of alkaline phosphatases, enzyme hydrolyzable phosphorus (measured for the first time in streams), increased the phosphorus supply to organisms with high APA.

Sign in / Sign up

Export Citation Format

Share Document