A simple thermometer-based photothermometric assay for alkaline phosphatase activity based on target-induced nanoprobe generation

2020 ◽  
Vol 44 (41) ◽  
pp. 17753-17760
Author(s):  
Honghong Rao ◽  
Huiyi Huang ◽  
Xinyuan Zhang ◽  
Xin Xue ◽  
Mingyue Luo ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Prussian Blue ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Prussian Blue Nanoparticles

Alkaline phosphatase (ALP)-induced in situ generation of Prussian blue nanoparticles for photothermometric ALP detection.

Influence of Light on the Alkaline Phosphatase Activity of Selenastrum capricornutum (Chlorophyceae) in Streams

1985 ◽  
Vol 42 (2) ◽  
pp. 384-388 ◽  
Author(s):  
R. L. Klotz
Keyword(s):  
New York ◽  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
New York State ◽  
Selenastrum Capricornutum ◽  
Alkaline Phosphatases ◽  
Reactive Phosphorus ◽  
First Time

The alkaline phosphatase activity (APA) of Selenastrum capricomutum Printz incubated in situ in four streams in New York State was inversely related to total insolation. APA was not correlated with stream molybdate reactive phosphorus over the range of concentrations encountered. Selenastrum showed no diel cycle of APA. The phosphorus fraction made available by the activity of alkaline phosphatases, enzyme hydrolyzable phosphorus (measured for the first time in streams), increased the phosphorus supply to organisms with high APA.

In situ electrochemical analysis of alkaline phosphatase activity in 3D cell cultures

2020 ◽  
Vol 359 ◽  
pp. 136951
Author(s):  
Claudia Caviglia ◽  
Rodrigo Pimentel Carletto ◽  
Stefania De Roni ◽  
Yasmin Mohamed Hassan ◽  
Suhith Hemanth ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Cell Cultures ◽  
Alkaline Phosphatase Activity ◽  
Electrochemical Analysis ◽  
3D Cell Cultures

Does Algal–Bacterial Phosphorus Partitioning Vary Among Lakes? A Comparative Study of Orthophosphate Uptake and Alkaline Phosphatase Activity in Freshwater

1986 ◽  
Vol 43 (2) ◽  
pp. 311-318 ◽  
Author(s):  
David J. Currie ◽  
Ellen Bentzen ◽  
Jacob Kalff
Keyword(s):  
Alkaline Phosphatase ◽  
Comparative Study ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Size Class ◽  
Phosphorus Partitioning

In order to distinguish the activity of phytoplankton and bacterioplankton in 13 lakes of widely varying trophy, we size-fractionated orthophosphate uptake and alkaline phosphatase activity. In most lakes, orthophosphate uptake was consistently and overwhelmingly associated with the smallest particles. Based upon indicators of algal and bacterial presence in each size class, we inferred that the bacterioplankton are responsible for ≥95% of the orthophosphate uptake in situ, except in lakes that are not phosphorus-deficient. In contrast, a large portion of the alkaline phosphatase activity was free in solution (median 46%), and much of the remainder (median 44%) was apparently associated with algae.

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