Influence of Light on the Alkaline Phosphatase Activity of Selenastrum capricornutum (Chlorophyceae) in Streams

1985 ◽  
Vol 42 (2) ◽  
pp. 384-388 ◽  
Author(s):  
R. L. Klotz
Keyword(s):  
New York ◽  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
New York State ◽  
Selenastrum Capricornutum ◽  
Alkaline Phosphatases ◽  
Reactive Phosphorus ◽  
First Time

The alkaline phosphatase activity (APA) of Selenastrum capricomutum Printz incubated in situ in four streams in New York State was inversely related to total insolation. APA was not correlated with stream molybdate reactive phosphorus over the range of concentrations encountered. Selenastrum showed no diel cycle of APA. The phosphorus fraction made available by the activity of alkaline phosphatases, enzyme hydrolyzable phosphorus (measured for the first time in streams), increased the phosphorus supply to organisms with high APA.

A simple thermometer-based photothermometric assay for alkaline phosphatase activity based on target-induced nanoprobe generation

2020 ◽  
Vol 44 (41) ◽  
pp. 17753-17760
Author(s):  
Honghong Rao ◽  
Huiyi Huang ◽  
Xinyuan Zhang ◽  
Xin Xue ◽  
Mingyue Luo ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Prussian Blue ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Prussian Blue Nanoparticles

Alkaline phosphatase (ALP)-induced in situ generation of Prussian blue nanoparticles for photothermometric ALP detection.

scholarly journals Alkaline Phosphatase Activity of Leukocytes in Shock

Blood ◽  
1955 ◽  
Vol 10 (7) ◽  
pp. 730-734 ◽  
Author(s):  
FRANCESCO VACCARI ◽  
BENITO SABOTTO ◽  
ENRICO MANZINI
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Alkaline Phosphatases ◽  
General Metabolism

Abstract In animals and patients subjected to stress procedures and shock, there occurs simultaneously with the increase in general metabolism a rise of the cytoplasmic localizations of the alkaline phosphatases in granulocytes.

scholarly journals Potential inhibitors of rat tooth alkaline phosphatase studied by means of different histochemical techniques.

1979 ◽  
Vol 27 (5) ◽  
pp. 982-988 ◽  
Author(s):  
A Larsson ◽  
G Hasselgren
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Sodium Fluoride ◽  
Alkaline Phosphatase Activity ◽  
Staining Pattern ◽  
Alkaline Phosphatases ◽  
Phosphate Ion ◽  
Potential Inhibitors ◽  
Lead Pyrophosphate ◽  
Hydrolysis Of

Two histochemical methods for demonstration of alkaline phosphatase activity, a lead pyrophosphate- anda naphtholphosphate technique, were compared. Since different results may be due to methodological differences as well as different enzyme activities, the enzymatic hydrolysis of the naphtholphosphate was visualized both by means of an azo-dye coupler and by lead-capturing of the liberated phosphate ion. Various potential inhibitors of alkaline phosphatase activity (diphosphonate, D-penicillamine, and sodium fluoride) were also tested. The use of diphosphonate and D-penicillamine resulted in inhibited or reduced staining, which could mainly be explained by an interference by these compounds with components in the incubation media rather than with the enzyme itself. The addition of sodium fluoride had no effect on the naphtholphosphate staining pattern irrespective of capturing method, whereas the odontoblastic pyrophosphate splitting alkaline phosphatase appeared to be sensitive to sodium fluoride, suggesting the presence of two alkaline phosphatases in odontoblasts.

Studies on the morphology, biology and development of a strigeid cercaria (Cercaria X Baylis 1930)

Parasitology ◽  
1958 ◽  
Vol 48 (3-4) ◽  
pp. 312-335 ◽  
Author(s):  
David A. Erasmus
Keyword(s):  
Alkaline Phosphatase ◽  
Life Cycle ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Glycogen Storage ◽  
Free Swimming ◽  
Time Of Infection ◽  
Histochemical Tests ◽  
First Time ◽  
Host Tissues

1. Cercaria X (Baylis & Taylor, 1930) and its sporocyst have been redescribed with some additions to the original descriptions.2. The details of the development of the cercaria into a metacercaria of the diplostomulum type have been obtained and the anatomy of the mature metacercaria is described in detail for the first time. It is proposed to name the metacercaria Diplostomulum X. Development of the cercaria into a metacercaria takes from 40 to 90 days and the metacercariae have been shown to survive for a period of 544 days after the time of infection. This development has been obtained in the lenses of rats, frogs and a wide variety of fish. Development is not completed in any other region of the host's body.3.The effect of the sporocyst, migration of the cercariae, and the metacercaria on the appropriate host tissues has been considered. All these stages produce extensive changes in the host tissues.4. Histochemical tests have shown the presence of alkaline phosphatase activity in the cuticle of the sporocyst. The glycogen storage regions of the cercaria are described and it has been demonstrated that the caudal bodies in the tail-stem contain large amounts of glycogen which are utilized during the free-swimming period.5. Attempts at the completion of the life cycle were unsuccessful.

In situ electrochemical analysis of alkaline phosphatase activity in 3D cell cultures

2020 ◽  
Vol 359 ◽  
pp. 136951
Author(s):  
Claudia Caviglia ◽  
Rodrigo Pimentel Carletto ◽  
Stefania De Roni ◽  
Yasmin Mohamed Hassan ◽  
Suhith Hemanth ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Cell Cultures ◽  
Alkaline Phosphatase Activity ◽  
Electrochemical Analysis ◽  
3D Cell Cultures
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