In Situ Monitoring Reactive Oxygen Species Released by Single Cells Using Scanning Electrochemical Microscopy with Self-Designed Multi-Potential Step Waveform

2021 ◽  
pp. 139638
Author(s):  
Tao Wu ◽  
Xin Ning ◽  
Qiang Xiong ◽  
Fan Zhang ◽  
Pingang He
Keyword(s):  
Reactive Oxygen Species ◽  
Single Cells ◽  
Reactive Oxygen ◽  
Scanning Electrochemical Microscopy ◽  
In Situ Monitoring ◽  
Oxygen Species ◽  
Potential Step ◽  
Electrochemical Microscopy

Redox reactions of reactive oxygen species in aqueous solutions as the probe for scanning electrochemical microscopy of single live T24 cells

2010 ◽  
Vol 88 (6) ◽  
pp. 569-576 ◽  
Author(s):  
Xiaocui Zhao ◽  
Mengni Zhang ◽  
Yitao Long ◽  
Zhifeng Ding
Keyword(s):  
Reactive Oxygen Species ◽  
Hydrogen Peroxide ◽  
Aqueous Solutions ◽  
Redox Reactions ◽  
Reactive Oxygen ◽  
Scanning Electrochemical Microscopy ◽  
Electrochemical Technique ◽  
Oxygen Species ◽  
T24 Cells ◽  
Electrochemical Microscopy

The redox reactions of two main components of reactive oxygen species (ROS), superoxide and hydrogen peroxide, along with oxygen in aqueous solutions were investigated using a conventional electrochemical technique, differential pulse voltammetry (DPV). Superoxide undergoes oxidation at a Pt working electrode biased at 0.055 V versus Ag/AgCl, while hydrogen peroxide can be oxidized and reduced at 0.817 and –0.745 V, respectively. Oxygen in the solutions is reduced at the electrode with an applied potential of –0.455 V. Based on these results, hydrogen peroxide and superoxide released from live cells can be successfully monitored, identified, and mapped using scanning electrochemical microscopy (SECM) at different potentials. Single human bladder (T24) cells were imaged using a 5 μm diameter SECM probe biased at –0.400, –0.600, and –0.800 V. Oxygen reduction that seems an interference can be discriminated from that of hydrogen peroxide by means of SECM.

scholarly journals Insights into the Photocatalytic Bacterial Inactivation by Flower-Like Bi2WO6 under Solar or Visible Light, Through in Situ Monitoring and Determination of Reactive Oxygen Species (ROS)

Water ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 1099 ◽  
Author(s):  
Minoo Karbasi ◽  
Fathallah Karimzadeh ◽  
Keyvan Raeissi ◽  
Sami Rtimi ◽  
John Kiwi ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Visible Light ◽  
Morphological Characteristics ◽  
Reactive Oxygen ◽  
In Situ Monitoring ◽  
Inactivation Kinetics ◽  
Oxygen Species ◽  
Bacterial Inactivation ◽  
X Ray

This study addresses the visible light-induced bacterial inactivation kinetics over a Bi2WO6 synthesized catalyst. The systematic investigation was undertaken with Bi2WO6 prepared by the complexation of Bi with acetic acid (carboxylate) leading to a flower-like morphology. The characterization of the as-prepared Bi2WO6 was carried out by X-ray diffraction (XRD), scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), specific surface area (SSA), and photoluminescence (PL). Under low intensity solar light (<48 mW/cm2), complete bacterial inactivation was achieved within two hours in the presence of the flower-like Bi2WO6, while under visible light, the synthesized catalyst performed better than commercial TiO2. The in situ interfacial charge transfer and local pH changes between Bi2WO6 and bacteria were monitored during the bacterial inactivation. Furthermore, the reactive oxygen species (ROS) were identified during Escherichia coli inactivation mediated by appropriate scavengers. The ROS tests alongside the morphological characteristics allowed the proposition of the mechanism for bacterial inactivation. Finally, recycling of the catalyst confirmed the stable nature of the catalyst presented in this study.

Scanning Electrochemical Microscopy Studies of Glutathione-Modified Surfaces. An Erasable and Sensitive-to-Reactive Oxygen Species Surface

Langmuir ◽  
2011 ◽  
Vol 27 (17) ◽  
pp. 11206-11211 ◽  
Author(s):  
Alina Latus ◽  
Jean-Marc Noël ◽  
Elena Volanschi ◽  
Corinne Lagrost ◽  
Philippe Hapiot
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Scanning Electrochemical Microscopy ◽  
Oxygen Species ◽  
Modified Surfaces ◽  
Electrochemical Microscopy

scholarly journals Reactive oxygen species formed in organic lithium–oxygen batteries

2016 ◽  
Vol 18 (16) ◽  
pp. 10774-10780 ◽  
Author(s):  
Patrick Schwager ◽  
Saustin Dongmo ◽  
Daniela Fenske ◽  
Gunther Wittstock
Keyword(s):  
Reactive Oxygen Species ◽  
Fluorescence Microscopy ◽  
Reactive Oxygen ◽  
Scanning Electrochemical Microscopy ◽  
Oxygen Species ◽  
Organic Electrolytes ◽  
Lithium Oxygen Batteries ◽  
Electrochemical Microscopy ◽  
Discharge Reaction

The generation of reactive oxygen species has been assumed to occur during the charging reaction of lithium-oxygen batteries with organic electrolytes. Here we show independently by fluorescence microscopy and scanning electrochemical microscopy that superoxide is also formed and released into the solution during the discharge reaction.

scholarly journals A residue-free approach to water disinfection using catalytic in situ generation of reactive oxygen species

2021 ◽  
Author(s):  
Thomas Richards ◽  
Jonathan H. Harrhy ◽  
Richard J. Lewis ◽  
Alexander G. R. Howe ◽  
Grzegorz M. Suldecki ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Water Disinfection ◽  
Oxygen Species ◽  
In Situ Generation

scholarly journals Advances in the in situ analysis of reactive oxygen species (ROS)

2021 ◽  
Vol 77 (8) ◽  
pp. 529-535
Author(s):  
Maja Szelągowska ◽  
Justyna Skrzypek ◽  
Maciej Gawlik
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
In Situ Analysis ◽  
Oxygen Species
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