Production of intracellular lipids from thermally hydrolyzed waste sludge by oleaginous yeast for energy and resource recovery

2022 ◽  
Vol 252 ◽  
pp. 115129
Author(s):  
Lei Wen ◽  
Xiao-yan Li
Keyword(s):  
Oleaginous Yeast ◽  
Resource Recovery ◽  
Intracellular Lipids ◽  
Waste Sludge

The isocitrate dehydrogenase gene of oleaginous yeast Lipomyces starkeyi is linked to lipid accumulation

2009 ◽  
Vol 55 (9) ◽  
pp. 1062-1069 ◽  
Author(s):  
Wei Tang ◽  
Sufang Zhang ◽  
Qian Wang ◽  
Haidong Tan ◽  
Zongbao Kent Zhao
Keyword(s):  
Isocitrate Dehydrogenase ◽  
Lipid Accumulation ◽  
Oleaginous Yeast ◽  
Phylogenetic Analyses ◽  
Dry Mass ◽  
Strain Engineering ◽  
Pcr Analysis ◽  
Lipomyces Starkeyi ◽  
Intracellular Lipids ◽  
Rapid Amplification

The oleaginous yeast Lipomyces starkeyi can accumulate intracellular lipids to over 60% of its cell dry mass under a nitrogen-limited condition. We showed that extracellular and intracellular citrate concentrations of L. starkeyi AS 2.1560 increased and the nicotinamide adenine dinucleotide – isocitrate dehydrogenase (NAD+–IDH) activity decreased at the beginning of the lipid accumulation, suggesting that the attenuation of the NAD+–IDH activity might initiate lipid storage. We next cloned the IDH gene by the methods of degenerate PCR and rapid amplification of cDNA ends. Phylogenetic analyses of the evolutionary relationships among LsIDH1, LsIDH2, and other yeast NAD+–IDHs revealed that the L. starkeyi IDH had a closer relationship with the IDHs of Yarrowia lipolytica . Further real-time PCR analysis showed that the expression levels of both LsIDH1 and LsIDH2 decreased concurrently with the evolution of cellular lipids. Our data should be valuable for understanding the biology of oleaginous yeasts and for further strain engineering of L. starkeyi.

scholarly journals Establishing CRISPR/Cas9 in Lipomyces starkeyi

2019 ◽  
Vol 2 (2) ◽  
pp. 51-52
Author(s):  
Zoe Lau ◽  
David Stuart ◽  
Bonnie Mcneil
Keyword(s):  
Protein Expression ◽  
Genome Editing ◽  
Oleaginous Yeast ◽  
Lipomyces Starkeyi ◽  
Guide Rna ◽  
Intracellular Lipids ◽  
Dry Cell Weight ◽  
Cas9 Protein ◽  
High Concentrations ◽  
Dry Cell

The goal of this project was to adapt the Yarrowia lipolytica plasmid based CRISPR/Cas9 system for usage in Lipomyces starkeyi. Lipomyces starkeyi is an oleaginous yeast, which synthesizes and stores high amounts of intracellular lipids. This specific yeast can store lipids at concentrations higher than 60% of its dry cell weight. Due to these high concentrations of lipids, L. starkeyi is a desired organism for the production of biofuels and other oleochemicals. However, there is a lack of knowledge and of genetic tools when trying to engineer the cells to produce these lipids for our use. The genome editing tool, CRISPR/Cas9 is efficient and simple, therefore desirable for the engineering of L. starkeyi. The goal was achieved by replacing the Y. lipolytica promoter with a L. starkeyi promoter, inserting guide RNA, as well as confirming cas9 protein expression.

Direct Methanolysis of Oleaginous Yeast Biomass (Pseudozyma parantarctica) to Microbial Biodiesel

2017 ◽  
Vol 753 ◽  
pp. 259-263
Author(s):  
Atsdawut Areesirisuk ◽  
Chiu Hsia Chiu ◽  
Tsair Bor Yen ◽  
Jia Hsin Guo
Keyword(s):  
Oleaginous Yeast ◽  
Methyl Esters ◽  
Lipid Extraction ◽  
Cetane Number ◽  
Acid Catalyst ◽  
Biodiesel Production ◽  
Intracellular Lipids ◽  
Yeast Biomass ◽  
Cellular Lipids ◽  
Astm D6751

In this study, intracellular lipids of a novel oleaginous biomass of P. parantarctica were converted to biodiesel directly using simple acid catalyst methanolysis. The optimum condition of this method was investigated. Under optimum conditions (0.1 M H2SO4, 10 h reaction time, 65°C reaction temperature, and 1:20 (w/v) biomass-to-methanol ratio), the yield of crude biodiesel was 93.18 ± 2.09% based on total cellular lipids. The composition of crude biodiesel was C16:C18 fatty acid methyl esters (FAMEs) for 91.91%. Especially, the C18:1 methyl ester was the main FAME (47.10%). In addition, the result showed that this technique could produce the microbial biodiesel from biomass containing high free fatty acids (FFAs) without soap formation. The predicted cetane number and kinematic viscosity of biodiesel were characterized according to ASTM D6751 and EN 14214 standards. Our results indicated that this process produces a good quality biodiesel. Moreover, it can decrease the manufacturing costs of microbial biodiesel production from oleaginous yeast biomass without cell disruption and lipid extraction.

scholarly journals Identification of oleaginous yeast strains able to accumulate high intracellular lipids when cultivated in alkaline pretreated corn stover

2014 ◽  
Vol 98 (17) ◽  
pp. 7645-7657 ◽  
Author(s):  
Irnayuli R. Sitepu ◽  
Mingjie Jin ◽  
J. Enrique Fernandez ◽  
Leonardo da Costa Sousa ◽  
Venkatesh Balan ◽  
...  
Keyword(s):  
Corn Stover ◽  
Oleaginous Yeast ◽  
Pretreated Corn Stover ◽  
Intracellular Lipids ◽  
Yeast Strains

Resource recovery treatment of waste sludge using a solubilizing reagent

2007 ◽  
Vol 9 (1) ◽  
pp. 34-39 ◽  
Author(s):  
Toshiyuki Nomura ◽  
Shunsuke Araki ◽  
Takanori Nagao ◽  
Yasuhiro Konishi
Keyword(s):  
Resource Recovery ◽  
Waste Sludge ◽  
Recovery Treatment

Increasing the fatigue strength of high-strength steel parts by repeated treatment with shot peening

2020 ◽  
pp. 79-82
Author(s):  
G.N. Kravchenko ◽  
K.G. Kravchenko
Keyword(s):  
Fatigue Strength ◽  
Shot Peening ◽  
High Strength Steel ◽  
High Strength ◽  
Resource Recovery ◽  
Repeated Treatment ◽  
Multiple Processing

The effectiveness of multiple hardening by shot peening of samples made of «30ХГСН2А» high-strength steel to increase their fatigue strength is experimentally established. Repeated hardenings allow not only to restore the original durability and even significantly increase it. Keywords fatigue strength, durability, resource recovery, multiple processing by shot peening, repeated hardening, high-strength steel. [email protected]

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