Cell death signaling and morphology in chemical-treated tobacco BY-2 suspension cultured cells

AbstractThe receptor interacting serine/threonine kinase1 and 3 (RIPK1, RIPK3) are regulators of cell death and survival. RIPK1 kinase activity is required for necroptosis and apoptosis, while its scaffolding function is necessary for survival. Although both proteins can mediate apoptosis, RIPK1 and RIPK3 are most well-known for their role in the execution of necroptosis via the mixed lineage domain like pseudokinase. Necroptosis is a caspase-independent regulated cell death program which was first described in cultured cells with unknown physiologic relevance in the liver. Many recent reports have suggested that RIPK1 and/or RIPK3 participate in liver disease pathogenesis and cell death. Notably, both proteins have been shown to mediate inflammation independent of cell death. Whether necroptosis occurs in hepatocytes, and how it is executed in the presence of an intact caspase machinery is controversial. In spite of this controversy, it is evident that RIPK1 and RIPK3 participate in many experimental liver disease models. Therefore, in addition to cell death signaling, their necroptosis-independent role warrants further examination.

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Cell death associated release of volatile organic sulphur compounds with antioxidant properties in chemical-challenged tobacco BY-2 suspension cultured cells

Journal of Plant Physiology ◽

10.1016/j.jplph.2020.153223 ◽

2020 ◽

Vol 251 ◽

pp. 153223

Author(s):

Elena T. Iakimova ◽

Zhenia P. Yordanova ◽

Simona M. Cristescu ◽

Frans F.M. Harren ◽

Ernst J. Woltering

Keyword(s):

Cell Death ◽

Cultured Cells ◽

Antioxidant Properties ◽

Sulphur Compounds ◽

Suspension Cultured Cells ◽

Organic Sulphur ◽

Volatile Organic

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Salt stress induces programmed cell death in Thellungiella halophila suspension-cultured cells

Journal of Plant Physiology ◽

10.1016/j.jplph.2010.03.008 ◽

2010 ◽

Vol 167 (14) ◽

pp. 1145-1151 ◽

Cited By ~ 41

Author(s):

Jin Wang ◽

Xinrong Li ◽

Yubing Liu ◽

Xin Zhao

Keyword(s):

Cell Death ◽

Salt Stress ◽

Programmed Cell Death ◽

Cultured Cells ◽

Thellungiella Halophila ◽

Suspension Cultured Cells

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DNA nick end labeling: a reliable marker for apoptosis?

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100141184 ◽

1995 ◽

Vol 53 ◽

pp. 962-963

Author(s):

Anne F. Bushnell ◽

Sarah Webster ◽

Lynn S. Perlmutter

Keyword(s):

Cell Death ◽

Cultured Cells ◽

Apoptotic Cell ◽

Morphological Characteristics ◽

Detection Methods ◽

Tissue Preparation ◽

Preparation Methods ◽

Dna Laddering ◽

Disease States ◽

Reliable Marker

Apoptosis, or programmed cell death, is an important mechanism in development and in diverse disease states. The morphological characteristics of apoptosis were first identified using the electron microscope. Since then, DNA laddering on agarose gels was found to correlate well with apoptotic cell death in cultured cells of dissimilar origins. Recently numerous DNA nick end labeling methods have been developed in an attempt to visualize, at the light microscopic level, the apoptotic cells responsible for DNA laddering.The present studies were designed to compare various tissue processing techniques and staining methods to assess the occurrence of apoptosis in post mortem tissue from Alzheimer's diseased (AD) and control human brains by DNA nick end labeling methods. Three tissue preparation methods and two commercial DNA nick end labeling kits were evaluated: the Apoptag kit from Oncor and the Biotin-21 dUTP 3' end labeling kit from Clontech. The detection methods of the two kits differed in that the Oncor kit used digoxigenin dUTP and anti-digoxigenin-peroxidase and the Clontech used biotinylated dUTP and avidinperoxidase. Both used 3-3' diaminobenzidine (DAB) for final color development.

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