Maternal serum PFOA concentration and DNA methylation in cord blood: A pilot study

Abstract Objectives Prior research has suggested that epigenetic mechanisms may underly associations between maternal caffeine intake and adverse childhood metabolic outcomes. We examined preconception and early pregnancy maternal caffeine exposure with DNA methylation (DNAm) patterns in the cord blood of 378 neonates. Methods DNAm was profiled by the Infinium MethylationEPIC BeadChip in women enrolled in the EAGeR Trial. Maternal serum was collected 1–2 cycles preconception and at 8 weeks gestation as was self-reported caffeinated beverage intake through standardized questionnaires or daily diaries. Serum caffeine, paraxanthine, and theobromine were measured by liquid chromatography mass spectrometry. We performed multivariable robust linear regression to assess associations between maternal caffeine and methylation β-values and Ingenuity Pathway Analysis to evaluate biologic implications. Results Preconceptionally, 65%, 21% and 7% reported any soda, coffee or tea intake, respectively with the majority consuming on average ≤ 1 serving/day. Preconception self-reported intake compared to no intake was associated with DNAm at cg09002832 near GLIS3 (false discovery rate [FDR] p = 0.036). No associations with self-reported intake were found during pregnancy. Preconception serum markers were not associated with individual CpG sites (FDR > 5%), though pregnancy theobromine (tertile 2 vs 1) was associated with DNAm at cg09460369 near RAB2A (FDR p = 0.012). Overlapping pathways for the top 100 CpG sites identified in the preconception intake and pregnancy theobromine analyses elucidated cell cycle and lipid metabolism processes. Conclusions Few differences in DNAm were identified in association with maternal caffeine intake in this low consumption population. DNAm changes from preconception caffeine or pregnancy theobromine exposure may be linked to signaling networks involving lipid metabolism, but further research among women with higher caffeine and theobromine exposure is warranted. Funding Sources Epidemiology Branch, Division of Intramural Population Health Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD.

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Global and single gene DNA methylation in umbilical cord blood cells after elective caesarean: a pilot study

European Journal of Obstetrics & Gynecology and Reproductive Biology ◽

10.1016/j.ejogrb.2014.05.038 ◽

2014 ◽

Vol 179 ◽

pp. 121-124 ◽

Cited By ~ 11

Author(s):

Maximilian B. Franz ◽

Mariella Poterauer ◽

Marie Elhenicky ◽

Susanne Stary ◽

Peter Birner ◽

...

Keyword(s):

Dna Methylation ◽

Pilot Study ◽

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Blood Cells ◽

Single Gene ◽

Cord Blood Cells ◽

Umbilical Cord Blood Cells

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Perfluorinated compounds in maternal serum and cord blood from selected areas of South Africa: results of a pilot study

Journal of Environmental Monitoring ◽

10.1039/b924420d ◽

2010 ◽

Vol 12 (6) ◽

pp. 1355 ◽

Cited By ~ 79

Author(s):

Linda Hanssen ◽

Halina Röllin ◽

Jon Øyvind Odland ◽

Morten K. Moe ◽

Torkjel M. Sandanger

Keyword(s):

South Africa ◽

Pilot Study ◽

Cord Blood ◽

Perfluorinated Compounds ◽

Maternal Serum

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Abstract #91: Comparison of Cord Blood Insulin in Large-for-Gestational-Age Infants and Appropriate-for-Age Filipino Infants of Nondiabetic Mothers: A Pilot Study

Endocrine Practice ◽

10.1016/s1530-891x(20)46363-3 ◽

2004 ◽

Vol 10 ◽

pp. 31

Author(s):

Florence M. Amorado-Santos ◽

Maria Honolina S. Gomez ◽

Maria Victoria R. Olivares ◽

Zayda N. Gamilla

Keyword(s):

Pilot Study ◽

Cord Blood ◽

Gestational Age ◽

Large For Gestational Age ◽

Blood Insulin

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DNA methylation analyses in a pilot study of patients with borderline personality disorder

10.26226/morressier.5785edc4d462b80296c99086 ◽

2016 ◽

Author(s):

Emese Kruk

Keyword(s):

Dna Methylation ◽

Borderline Personality Disorder ◽

Pilot Study ◽

Personality Disorder ◽

Borderline Personality

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Treatment of Children With Autistic Spectrum Disorder With Autologous Umbilical Cord Blood, a Pilot Study

Case Medical Research ◽

10.31525/ct1-nct04243382 ◽

2020 ◽

Author(s):

Keyword(s):

Pilot Study ◽

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Autistic Spectrum Disorder ◽

Spectrum Disorder ◽

Autistic Spectrum

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Maternal Vitamin D and Newborn Telomere Length

Nutrients ◽

10.3390/nu13062012 ◽

2021 ◽

Vol 13 (6) ◽

pp. 2012

Author(s):

Lisa Daneels ◽

Dries S. Martens ◽

Soumia Arredouani ◽

Jaak Billen ◽

Gudrun Koppen ◽

...

Keyword(s):

Vitamin D ◽

Cord Blood ◽

Telomere Length ◽

First Trimester ◽

Maternal Serum ◽

Adverse Outcomes ◽

Dietary Vitamin ◽

Maternal Vitamin ◽

Vitamin D Intake ◽

Diet Supplements

Nutrition is important during pregnancy for offspring health. Gestational vitamin D intake may prevent several adverse outcomes and might have an influence on offspring telomere length (TL). In this study, we want to assess the association between maternal vitamin D intake during pregnancy and newborn TL, as reflected by cord blood TL. We studied mother–child pairs enrolled in the Maternal Nutrition and Offspring’s Epigenome (MANOE) cohort, Leuven, Belgium. To calculate the dietary vitamin D intake, 108 women were asked to keep track of their diet using the seven-day estimated diet record (EDR) method. TL was assessed in 108 cord blood using a quantitative real-time PCR method. In each trimester of pregnancy, maternal serum 25-hydroxyvitamin D (25-OHD) concentration was measured. We observed a positive association (β = 0.009, p-value = 0.036) between newborn average relative TL and maternal vitamin D intake (diet + supplement) during the first trimester. In contrast, we found no association between average relative TL of the newborn and mean maternal serum 25-OHD concentrations during pregnancy. To conclude, vitamin D intake (diet + supplements), specifically during the first trimester of pregnancy, is an important factor associated with TL at birth.

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Birthweight DNA methylation signatures in infant saliva

Clinical Epigenetics ◽

10.1186/s13148-021-01053-1 ◽

2021 ◽

Vol 13 (1) ◽

Author(s):

Chiara Moccia ◽

Maja Popovic ◽

Elena Isaevska ◽

Valentina Fiano ◽

Morena Trevisan ◽

...

Keyword(s):

Dna Methylation ◽

Cord Blood ◽

Gestational Age ◽

Low Birthweight ◽

Continuous Variable ◽

Linear Regression Models ◽

Association Analyses ◽

Cpg Sites ◽

Adverse Health Outcomes ◽

The Look

Abstract Background Low birthweight has been repeatedly associated with long-term adverse health outcomes and many non-communicable diseases. Our aim was to look-up cord blood birthweight-associated CpG sites identified by the PACE Consortium in infant saliva, and to explore saliva-specific DNA methylation signatures of birthweight. Methods DNA methylation was assessed using Infinium HumanMethylation450K array in 135 saliva samples collected from children of the NINFEA birth cohort at an average age of 10.8 (range 7–17) months. The association analyses between birthweight and DNA methylation variations were carried out using robust linear regression models both in the exploratory EWAS analyses and in the look-up of the PACE findings in infant saliva. Results None of the cord blood birthweight-associated CpGs identified by the PACE Consortium was associated with birthweight when analysed in infant saliva. In saliva EWAS analyses, considering a false discovery rate p-values < 0.05, birthweight as continuous variable was associated with DNA methylation in 44 CpG sites; being born small for gestational age (SGA, lower 10th percentile of birthweight for gestational age according to WHO reference charts) was associated with DNA methylation in 44 CpGs, with only one overlapping CpG between the two analyses. Despite no overlap with PACE results at the CpG level, two of the top saliva birthweight CpGs mapped at genes associated with birthweight with the same direction of the effect also in the PACE Consortium (MACROD1 and RPTOR). Conclusion Our study provides an indication of the birthweight and SGA epigenetic salivary signatures in children around 10 months of age. DNA methylation signatures in cord blood may not be comparable with saliva DNA methylation signatures at about 10 months of age, suggesting that the birthweight epigenetic marks are likely time and tissue specific.

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Associations of circulating folate, vitamin B12 and homocysteine concentrations in early pregnancy and cord blood with epigenetic gestational age: the Generation R Study

Clinical Epigenetics ◽

10.1186/s13148-021-01065-x ◽

2021 ◽

Vol 13 (1) ◽

Author(s):

Giulietta S. Monasso ◽

Leanne K. Küpers ◽

Vincent W. V. Jaddoe ◽

Sandra G. Heil ◽

Janine F. Felix

Keyword(s):

Dna Methylation ◽

Vitamin B12 ◽

Cord Blood ◽

Gestational Age ◽

Fetal Development ◽

Maternal Plasma ◽

Plasma Homocysteine ◽

Training Population ◽

Epigenetic Aging ◽

Pregnancy Dating

Abstract Background Circulating folate, vitamin B12 and homocysteine concentrations during fetal development have been associated with health outcomes in childhood. Changes in fetal DNA methylation may be an underlying mechanism. This may be reflected in altered epigenetic aging of the fetus, as compared to chronological aging. The difference between gestational age derived in clinical practice and gestational age predicted from neonatal DNA methylation data is referred to as gestational age acceleration. Differences in circulating folate, vitamin B12 and homocysteine concentrations during fetal development may be associated with gestational age acceleration. Results Up to 1346 newborns participating in the Generation R Study, a population-based prospective cohort study, had both cord blood DNA methylation data available and information on plasma folate, serum total and active B12 and plasma homocysteine concentrations, measured in early pregnancy and/or in cord blood. A subgroup of 380 newborns had mothers with optimal pregnancy dating based on a regular menstrual cycle and a known date of last menstrual period. For comparison, gestational age acceleration was calculated based the method of both Bohlin and Knight. In the total study population, which was more similar to Bohlin’s training population, one standard deviation score (SDS) higher maternal plasma homocysteine concentrations was nominally associated with positive gestational age acceleration [0.07 weeks, 95% confidence interval (CI) 0.02, 0.13] by Bohlin’s method. In the subgroup with pregnancy dating based on last menstrual period, the method that was also used in Knight’s training population, one SDS higher cord serum total and active B12 concentrations were nominally associated with negative gestational age acceleration [(− 0.16 weeks, 95% CI − 0.30, − 0.02) and (− 0.15 weeks, 95% CI − 0.29, − 0.01), respectively] by Knight’s method. Conclusions We found some evidence to support associations of higher maternal plasma homocysteine concentrations with positive gestational age acceleration, suggesting faster epigenetic than clinical gestational aging. Cord serum vitamin B12 concentrations may be associated with negative gestational age acceleration, indicating slower epigenetic than clinical gestational aging. Future studies could examine whether altered fetal epigenetic aging underlies the associations of circulating homocysteine and vitamin B12 blood concentrations during fetal development with long-term health outcomes.

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