Dissimilatory nitrate/nitrite reduction to ammonium (DNRA) pathway dominates nitrate reduction processes in rhizosphere and non-rhizosphere of four fertilized farmland soil

AbstractTo overcome the problem that ferrous complexes are easily oxidized by O2 and then lose NO binding ability in the chemical absorption-biological reduction (CABR) process, cobalt(II)-histidine [Co(II)His] was proposed as an alternative. To evaluate the applicability of Co(II)His, the effects of CoHis absorbent on the aerobic denitrification by Paracoccus versutus LYM were investigated. Results indicated that His significantly promoted nitrite reduction. The inhibition effects of CoHis absorbent could be substantially alleviated by increasing the initial His/Co2+ to 4 or higher. CoHis with concentrations of 4, 8, 12, 16 and 20 mM presented no distinct effect on nitrite reduction, but slightly inhibited the reduction of nitrate, resulting in longer lag of nitrate reduction, and obviously promoted the growth of strain LYM. In the presence of 5, 10, 15 and 20 mM CoHis absorbent, the main denitrification product was N2 (not less than 95.0%). This study is of significance in verifying the applicability of Co(II)His in the CABR process, and provides a referable CoHis absorbent concentration as 20 mM with an initial His/Co2+ of 4 for the future experiments.

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Variations of dissimilatory nitrate reduction processes along reclamation chronosequences in Chongming Island, China

Soil and Tillage Research ◽

10.1016/j.still.2020.104815 ◽

2021 ◽

Vol 206 ◽

pp. 104815

Author(s):

Yinghui Jiang ◽

Guoyu Yin ◽

Lijun Hou ◽

Min Liu ◽

Dengzhou Gao ◽

...

Keyword(s):

Nitrate Reduction ◽

Chongming Island ◽

Reduction Processes ◽

Dissimilatory Nitrate Reduction

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Biogenic Fe(II-III) Hydroxycarbonate Green Rust Enhances Nitrate Removal and Decreases Ammonium Selectivity during Heterotrophic Denitrification

Minerals ◽

10.3390/min10090818 ◽

2020 ◽

Vol 10 (9) ◽

pp. 818

Author(s):

Georges Ona-Nguema ◽

Delphine Guerbois ◽

Céline Pallud ◽

Jessica Brest ◽

Mustapha Abdelmoula ◽

...

Keyword(s):

Nitrate Reduction ◽

Nitrate Removal ◽

Green Rust ◽

Nitrite Reduction ◽

Nitrite Accumulation ◽

Bacterial Reduction ◽

Water Treatment Process ◽

Nitrite Production ◽

Ammonium Production ◽

Biogenic Iron

Nitrification-denitrification is the most widely used nitrogen removal process in wastewater treatment. However, this process can lead to undesirable nitrite accumulation and subsequent ammonium production. Biogenic Fe(II-III) hydroxycarbonate green rust has recently emerged as a candidate to reduce nitrite without ammonium production under abiotic conditions. The present study investigated whether biogenic iron(II-III) hydroxycarbonate green rust could also reduce nitrite to gaseous nitrogen during bacterial nitrate reduction. Our results showed that biogenic iron(II-III) hydroxycarbonate green rust could efficiently decrease the selectivity of the reaction towards ammonium during heterotrophic nitrate reduction by native wastewater-denitrifying bacteria and by three different species of Shewanella: S. putrefaciens ATCC 12099, S. putrefaciens ATCC 8071 and S. oneidensis MR-1. Indeed, in the absence of biogenic hydroxycarbonate green rust, bacterial reduction of nitrate converted 11–42% of the initial nitrate into ammonium, but this value dropped to 1–28% in the presence of biogenic hydroxycarbonate green rust. Additionally, nitrite accumulation did not exceed the 2–13% in the presence of biogenic hydroxycarbonate green rust, versus 0–28% in its absence. Based on those results that enhance the extent of denitrification of about 60%, the study proposes a water treatment process that couples the bacterial nitrite production with the abiotic nitrite reduction by biogenic green rust.

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Implications of Limited Thermophilicity of Nitrite Reduction for Control of Sulfide Production in Oil Reservoirs

Applied and Environmental Microbiology ◽

10.1128/aem.00599-16 ◽

2016 ◽

Vol 82 (14) ◽

pp. 4190-4199 ◽

Cited By ~ 32

Author(s):

Tekle Tafese Fida ◽

Chuan Chen ◽

Gloria Okpala ◽

Gerrit Voordouw

Keyword(s):

Nitrate Reduction ◽

Nitrite Reduction ◽

Oil Fields ◽

Microbial Consortia ◽

Subsequent Reduction ◽

Content Type ◽

Reduction Activity ◽

Pure Cultures ◽

Nitrate And Nitrite ◽

Reducing Bacteria

ABSTRACTNitrate reduction to nitrite in oil fields appears to be more thermophilic than the subsequent reduction of nitrite. Concentrated microbial consortia from oil fields reduced both nitrate and nitrite at 40 and 45°C but only nitrate at and above 50°C. The abundance of thenirSgene correlated with mesophilic nitrite reduction activity.ThaueraandPseudomonaswere the dominant mesophilic nitrate-reducing bacteria (mNRB), whereasPetrobacterandGeobacilluswere the dominant thermophilic NRB (tNRB) in these consortia. The mNRBThauerasp. strain TK001, isolated in this study, reduced nitrate and nitrite at 40 and 45°C but not at 50°C, whereas the tNRBPetrobactersp. strain TK002 andGeobacillussp. strain TK003 reduced nitrate to nitrite but did not reduce nitrite further from 50 to 70°C. Testing of 12 deposited pure cultures of tNRB with 4 electron donors indicated reduction of nitrate in 40 of 48 and reduction of nitrite in only 9 of 48 incubations. Nitrate is injected into high-temperature oil fields to prevent sulfide formation (souring) by sulfate-reducing bacteria (SRB), which are strongly inhibited by nitrite. Injection of cold seawater to produce oil creates mesothermic zones. Our results suggest that preventing the temperature of these zones from dropping below 50°C will limit the reduction of nitrite, allowing more effective souring control.IMPORTANCENitrite can accumulate at temperatures of 50 to 70°C, because nitrate reduction extends to higher temperatures than the subsequent reduction of nitrite. This is important for understanding the fundamentals of thermophilicity and for the control of souring in oil fields catalyzed by SRB, which are strongly inhibited by nitrite.

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The synergetic and competitive mechanism and its dominant affecting factors of dissimilatory nitrate reduction processes: a review

Acta Ecologica Sinica ◽

10.5846/stxb201407181464 ◽

2016 ◽

Vol 36 (5) ◽

Cited By ~ 1

Author(s):

杨杉 YANG Shan ◽

吴胜军 WU Shengjun ◽

蔡延江 CAI Yanjiang ◽

周文佐 ZHOU Wenzuo ◽

朱同彬 ZHU Tongbin ◽

...

Keyword(s):

Nitrate Reduction ◽

Affecting Factors ◽

Reduction Processes ◽

Dissimilatory Nitrate Reduction ◽

Competitive Mechanism

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Nitrate reduction and nitrogenase activity in Spirillum lipoferum

Canadian Journal of Microbiology ◽

10.1139/m77-045 ◽

1977 ◽

Vol 23 (3) ◽

pp. 306-310 ◽

Cited By ~ 38

Author(s):

Carlos A. Neyra ◽

Peter Van Berkum

Keyword(s):

Nitrate Reduction ◽

Time Course ◽

Nitrogenase Activity ◽

Reductase Activity ◽

Nitrite Reduction ◽

Lag Phase ◽

Nitrite Accumulation ◽

Assimilatory Nitrate Reduction ◽

Nitrate And Nitrite ◽

Anaerobic Pretreatment

Nitrate and nitrite reduction under aerobic, microaerophillic, and anaerobic conditions was demonstrated in Spirillum lipoferum (ATCC 29145). Nitrite did not accumulate during assimilatory nitrate reduction in air. The nitrite produced during dissimilatory nitrate reduction accumulated in the medium but not in the cells. On exposure of the bacteria to nitrate and anaerobiosis, a low initial rate (lag) was followed by accelerated rates of nitrite accumulation. A 3-h anaerobic pretreatment, in the absence of nitrate, did not avoid the lag phase. No nitrate reductase activity (NRA) developed in the presence of chloramphenicol. The data suggest that induction of anaerobic NRA in S. lipoferum required nitrate and protein synthesis.Anaerobic N2ase activity by S. lipoferum was greatly stimulated in the presence of nitrate. The time course of nitrate reduction was coincidental with the pattern of nitrate-stimulated N2ase activity indicating that a relationship exists between these two processes.

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Glycerol-driven Denitratation: Process Kinetics, Microbial Ecology, and Operational Controls

10.1101/2021.09.25.461789 ◽

2021 ◽

Author(s):

Matthew P. Baideme ◽

Chenghua Long ◽

Luke T. Plante ◽

Jeffrey A. Starke ◽

Michael A. Butkus ◽

...

Keyword(s):

Organic Carbon ◽

Microbial Ecology ◽

Nitrate Reduction ◽

Carbon Sources ◽

Selective Reduction ◽

Nitrite Reduction ◽

Nitrite Accumulation ◽

Ammonium Oxidation ◽

Order Of Magnitude ◽

Nitrate And Nitrite

ABSTRACTDenitratation, the selective reduction of nitrate to nitrite, is a novel process when coupled with anaerobic ammonium oxidation (anammox) could achieve resource-efficient biological nitrogen removal of ammonium- and nitrate-laden waste streams. Using a fundamentally-based, first principles approach, this study optimized a stoichiometrically-limited, glycerol-driven denitratation process and characterized mechanisms supporting nitrite accumulation with results that aligned with expectations. Glycerol supported selective nitrate reduction to nitrite and near-complete nitrate conversion, indicating its viability in a denitratation system. Glycerol-supported specific rates of nitrate reduction (135.3 mg-N/g-VSS/h) were at least one order of magnitude greater than specific rates of nitrite reduction (14.9 mg-N/g-VSS/h), potentially resulting in transient nitrite accumulation and indicating glycerol’s superiority over other organic carbon sources in denitratation systems. pH and ORP inflection points in nitrogen transformation assays corresponded to maximum nitrite accumulation, indicating operational setpoints to prevent further nitrite reduction. Denitratation conditions supported enrichment of Thauera sp. as the dominant genus. Stoichiometric limitation of influent organic carbon, coupled with differential nitrate and nitrite reduction kinetics, optimized operational controls, and a distinctively enriched microbial ecology, was identified as causal in glycerol-driven denitratation.

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