Nutrient Removal in Sequential Batch Polishing Ponds

One of the main problems of waste stabilization ponds (WSP) is that they cannot remove nutrients when treating wastewater. Polishing ponds (PP) can efficiently remove nitrogen and phosphorus from effluents after efficient anaerobic pretreatment. It shown that the feasibility of nutrient removal is directly related to the pH that is established in the ponds. WSP normally operate at near neutral pH, but the biological processes that develop in PP tend to cause an elevation of pH and this, in turn, triggers the mechanisms of nutrient removal in ponds. In PP oxygen production by photosynthesis predominates over the oxidation of organic material. The net oxygen production has an equivalent CO2 consumption and this induces an increase in pH. The mechanism for nitrogen removal was identified as the desorption of ammonia from the liquid phase of the ponds. It was established that in ponds with a uniform concentration profile in the liquid phase the process developed in accordance with Fick’s law. The governing mechanism of phosphorus removal was precipitation with ions present in the wastewater, presumably calcium and magnesium. Polishing ponds can be operated with two different hydrodynamic regimes: flow-through (FTPP) and sequential batch (SBPP) ponds. The SBPP have the advantage that the pH elevation is more rapid, and that the final pH is higher.

Synergistic Co-Digestion of Microalgae and Primary Sludge to Enhance Methane Yield from Temperature-Phased Anaerobic Digestion

A two-stage temperature-phased mesophilic anaerobic digestion assay was carried out to study the interaction between various biological pretreatment conditions and the possible synergistic co-digestion of microalgae and primary sludge. The study of growth kinetics of the biochemical methane potential test revealed that a maximum of 36% increase in methane yield was observed from co-digestion of a substrate pretreated by thermophilic aerobic conditions (55 °C and HRT = 2 days) and an 8.3% increase was obtained from the anaerobic pretreated substrate (55 °C and HRT = 3 days). Moreover, no synergistic effects on methane yields were observed in co-digesting the substrate pretreated with high temperature (85 °C). The study also identified specific conditions in which interaction between biological pretreatment and co-digestion might substantially reduce methane yield. Careful optimization of operating conditions, both aerobic and anaerobic pretreatment at moderate thermophilic conditions, can be used as a biological pretreatment to enhance methane yield from the co-digestion of microalgae and primary sludge.

Bioreactor and wetland combinations

Wastewater treatment plants combining bioreactors and natural processes, designed to achievecost efficient treatment, are described and evaluated. The plants have a common generallayout: an anaerobic pretreatment, an aerated bioreactor, sedimentation with sludge return anda final sub-surface flow wetland treatment. Variations in this design, adaptations to variousapplications, process control strategies and sludge handling are discussed. Removalefficiencies obtained varies in the range: 96-99 % BOO7, 72-88 % COD, 92-96 % SS, 80-99% P, 37-91% N, where more advanced control yield higher efficiency. Thermo-tolerant fecalcoliform bacteria are typically removed by 99.9 %. Most of the nitrogen is removed in thebioreactors. Computer controlled aeration and sludge handling is required to obtain the hightotal nitrogen removal (> 80 %) Phosphorus can also be removed in the bioreactors andexported as sludge, or, more cost effectively, mainly removed in the wetland part of the plants.The cost efficiency of such treatment plants is good compared to alternative solutions.

Surface-to-surface biofilm transfer: a quick and reliable startup strategy for mixed culture microbial fuel cells

The startup of microbial fuel cells (MFCs) is known to be prone to failure or result in erratic performance impeding the research. The aim of this study was to advise a quick launch strategy for laboratory-scale MFCs that ensures steady operation performance in a short period of time. Different startup strategies were investigated and compared with membraneless single chamber MFCs. A direct surface-to-surface biofilm transfer (BFT) in an operating MFC proved to be the most efficient method. It provided steady power densities of 163 ± 13 mWm−2 4 days after inoculation compared to 58 ± 15 mWm−2 after 30 days following a conventional inoculation approach. The in situ BFT eliminates the need for microbial acclimation during startup and reduces performance fluctuations caused by shifts in microbial biodiversity. Anaerobic pretreatment of the substrate and addition of suspended enzymes from an operating MFC into the new MFC proved to have a beneficial effect on startup and subsequent operation. Polarization methods were applied to characterize the startup phase and the steady state operation in terms of power densities, internal resistance and power overshoot during biofilm maturation. Applying this method a well-working MFC can be multiplied into an array of identically performing MFCs.