Mechanism of recombinant bacteria adsorb UO22+ under culture condition

2021 ◽  
pp. 109920
Author(s):  
Yangzhen Shu ◽  
Shanshan Li ◽  
Jingxi Xie ◽  
Kexin Guo ◽  
Conghui Cheng ◽  
...  
Keyword(s):  
Culture Condition ◽  
Recombinant Bacteria

La rhétorique de la légitimité

2014 ◽  
Vol 32 (4) ◽  
pp. 348-361
Author(s):  
Hélène Casanova-Robin
Keyword(s):  
Culture Condition

Le traité De Principe rédigé par Giovanni Pontano dans la seconde moitié du Quattrocento vise à éduquer le futur souverain, Alphonse II et à lui offrir tous les outils pour conforter sa légitimité contestée par les barons napolitains. La rhétorique de l'éloge, conjuguée au discours didactique, sert ici la construction d'un idéal de souverain, appuyé sur les modèles antiques mais aussi nourri de références contemporaines, l'humaniste proposant une véritable parénèse vers l'accession à la sagesse, qui repose sur l'acquisition d'une solide culture, condition sine qua non pour que se développent les vertus. Mais cet apprentissage demeure incomplet si le prince ne sait pas en outre se doter d'une persona destinée précisément à faire apparaître ses qualités. L'attention portée au souverain en représentation, exposée d'après les modèles de l'orateur et de l'acteur, ouvre la voix à une nouvelle conception de l'exercice du pouvoir.

Retention and expression of recombinant plasmids in suspended and biofilm-bound bacteria degrading trichloroethene (TCE)

1997 ◽  
Vol 36 (10) ◽  
pp. 1-8 ◽  
Author(s):  
James D. Bryers ◽  
Robert R. Sharp
Keyword(s):  
Biofilm Reactor ◽  
Bacterial Biofilm ◽  
Laboratory Evaluation ◽  
Recombinant Bacteria ◽  
Suitable Host ◽  
Term Operation ◽  
Open Environment ◽  
Recombinant Microorganisms ◽  
Extracellular Polymer

Exposure of plasmid recombinant microorganisms to an open environment, either inadvertently or intentionally, requires research into those fundamental processes that govern plasmid retention, transfer and expression. In the open environment, a majority of the microbial activity occurs associated with an interface, within thin biological layers consisting of cells and their insoluble extracellular polymer, layers known as biofilms. Current toxic wastewater or wastegas treatment reactors exploit bacterial biofilm systems for certain system operating advantages. Using recombinant bacteria within a biofilm reactor to degrade xenobiotic wastes requires finding a suitable host to harbor and express the desired plasmid phenotype. Suitable host characteristics include: the ability to produce copious amounts of biofilm, resistance to waste-related injury and toxicity, and the ability to retain and express the desired plasmid during long term operation. This paper reports on a laboratory evaluation of factors governing plasmid retention and the expression of trichloroethene (TCE) degradative capacity in both suspended and biofilm cultures.

scholarly journals Detecting the Mechanism behind the Transition from Fixed Two-Dimensional Patterned Sika Deer (Cervus nippon) Dermal Papilla Cells to Three-Dimensional Pattern

2021 ◽  
Vol 22 (9) ◽  
pp. 4715
Author(s):  
Guanning Wei ◽  
Hongmei Sun ◽  
Haijun Wei ◽  
Tao Qin ◽  
Yifeng Yang ◽  
...  
Keyword(s):  
Culture Condition ◽  
Sika Deer ◽  
Three Dimensional ◽  
Cervus Nippon ◽  
Dermal Papilla ◽  
Growth Patterns ◽  
The Self ◽  
Dermal Papilla Cells ◽  
Aggregative Behavior ◽  
Cell Pattern

The hair follicle dermal papilla is critical for hair generation and de novo regeneration. When cultured in vitro, dermal papilla cells from different species demonstrate two distinguishable growth patterns under the conventional culture condition: a self-aggregative three dimensional spheroidal (3D) cell pattern and a two dimensional (2D) monolayer cell pattern, correlating with different hair inducing properties. Whether the loss of self-aggregative behavior relates to species-specific differences or the improper culture condition remains unclear. Can the fixed 2D patterned dermal papilla cells recover the self-aggregative behavior and 3D pattern also remains undetected. Here, we successfully constructed the two growth patterns using sika deer (Cervus nippon) dermal papilla cells and proved it was the culture condition that determined the dermal papilla growth pattern. The two growth patterns could transit mutually as the culture condition was exchanged. The fixed 2D patterned sika deer dermal papilla cells could recover the self-aggregative behavior and transit back to 3D pattern, accompanied by the restoration of hair inducing capability when the culture condition was changed. In addition, the global gene expressions during the transition from 2D pattern to 3D pattern were compared to detect the potential regulating genes and pathways involved in the recovery of 3D pattern and hair inducing capability.

scholarly journals Characterization and Site-specific Mutagenesis of the Calcium-binding Protein Oncomodulin Produced by Recombinant Bacteria

1989 ◽  
Vol 264 (6) ◽  
pp. 3470-3477
Author(s):  
J P MacManus ◽  
C M L Hutnik ◽  
B D Sykes ◽  
A G Szabo ◽  
T C Williams ◽  
...  
Keyword(s):  
Calcium Binding ◽  
Binding Protein ◽  
Calcium Binding Protein ◽  
Recombinant Bacteria ◽  
Site Specific

scholarly journals Chemically defined and xeno-free culture condition for human extended pluripotent stem cells

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Bei Liu ◽  
Shi Chen ◽  
Yaxing Xu ◽  
Yulin Lyu ◽  
Jinlin Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Culture Condition ◽  
Human Fibroblast ◽  
Culture System ◽  
Disease Modeling ◽  
Directed Differentiation

AbstractExtended pluripotent stem (EPS) cells have shown great applicative potentials in generating synthetic embryos, directed differentiation and disease modeling. However, the lack of a xeno-free culture condition has significantly limited their applications. Here, we report a chemically defined and xeno-free culture system for culturing and deriving human EPS cells in vitro. Xeno-free human EPS cells can be long-term and genetically stably maintained in vitro, as well as preserve their embryonic and extraembryonic developmental potentials. Furthermore, the xeno-free culturing system also permits efficient derivation of human EPS cells from human fibroblast through reprogramming. Our study could have broad utility in future applications of human EPS cells in biomedicine.

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