scholarly journals Bacterial populations in the rhizosphere of tobacco plants producing the quorum-sensing signals hexanoyl-homoserine lactone and 3-oxo-hexanoyl-homoserine lactone

2004 ◽  
Vol 51 (1) ◽  
pp. 19-29 ◽  
Author(s):  
Cathy D'Angelo-Picard ◽  
Denis Faure ◽  
Aurélien Carlier ◽  
Stéphane Uroz ◽  
Aurélie Raffoux ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Homoserine Lactone ◽  
Tobacco Plants ◽  
Bacterial Populations ◽  
Quorum Sensing Signals

scholarly journals Host modification of a bacterial quorum-sensing signal induces a phenotypic switch in bacterial symbionts

2017 ◽  
Vol 114 (40) ◽  
pp. E8488-E8497 ◽  
Author(s):  
Cleo Pietschke ◽  
Christian Treitz ◽  
Sylvain Forêt ◽  
Annika Schultze ◽  
Sven Künzel ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Bacterial Communities ◽  
Homoserine Lactone ◽  
Innate Immune ◽  
Expression Data ◽  
Phenotypic Switch ◽  
Host Colonization ◽  
Bacterial Quorum Sensing ◽  
Bacterial Quorum ◽  
Quorum Sensing Signals

Bacterial communities colonize epithelial surfaces of most animals. Several factors, including the innate immune system, mucus composition, and diet, have been identified as determinants of host-associated bacterial communities. Here we show that the early branching metazoan Hydra is able to modify bacterial quorum-sensing signals. We identified a eukaryotic mechanism that enables Hydra to specifically modify long-chain 3-oxo-homoserine lactones into their 3-hydroxy-HSL counterparts. Expression data revealed that Hydra’s main bacterial colonizer, Curvibacter sp., responds differentially to N-(3-hydroxydodecanoyl)-l-homoserine lactone (3OHC12-HSL) and N-(3-oxododecanoyl)-l-homoserine lactone (3OC12-HSL). Investigating the impacts of the different N-acyl-HSLs on host colonization elucidated that 3OHC12-HSL allows and 3OC12-HSL represses host colonization of Curvibacter sp. These results show that an animal manipulates bacterial quorum-sensing signals and that this modification leads to a phenotypic switch in the bacterial colonizers. This mechanism may enable the host to manipulate the gene expression and thereby the behavior of its bacterial colonizers.

Chemical identification of N -acyl homoserine lactone quorum-sensing signals produced by Sinorhizobium meliloti strains in defined medium

2003 ◽  
Vol 180 (6) ◽  
pp. 494-497 ◽  
Author(s):  
Max Teplitski ◽  
Anatol Eberhard ◽  
Matthew R. Gronquist ◽  
Mengsheng Gao ◽  
Jayne B. Robinson ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Sinorhizobium Meliloti ◽  
Homoserine Lactone ◽  
Defined Medium ◽  
Acyl Homoserine Lactone ◽  
Chemical Identification ◽  
Quorum Sensing Signals

scholarly journals So different and still so similar: The plant compound rosmarinic acid mimics bacterial homoserine lactone quorum sensing signals

2016 ◽  
Vol 9 (2) ◽  
pp. e1156832 ◽  
Author(s):  
Andrés Corral-Lugo ◽  
Abdelali Daddaoua ◽  
Alvaro Ortega ◽  
Manuel Espinosa-Urgel ◽  
Tino Krell
Keyword(s):  
Quorum Sensing ◽  
Rosmarinic Acid ◽  
Homoserine Lactone ◽  
Quorum Sensing Signals

scholarly journals Quorum-Sensing Signals and Quorum-Sensing Genes in Burkholderia vietnamiensis

2002 ◽  
Vol 184 (4) ◽  
pp. 1187-1191 ◽  
Author(s):  
Barbara-Ann Conway ◽  
E. P. Greenberg
Keyword(s):  
Quorum Sensing ◽  
Burkholderia Cepacia ◽  
Homoserine Lactone ◽  
Abundant Species ◽  
Open Reading Frame ◽  
Reading Frame ◽  
Burkholderia Vietnamiensis ◽  
Dna Fragment ◽  
Low Levels ◽  
Quorum Sensing Signals

ABSTRACT Acyl-homoserine lactone (acyl-HSL) quorum sensing is common to many Proteobacteria including a clinical isolate of Burkholderia cepacia. The B. cepacia isolate produces low levels of octanoyl-HSL. We have examined an environmental isolate of Burkholderia vietnamiensis. This isolate produced several acyl-HSLs. The most abundant species was decanoyl-HSL. Decanoyl-HSL in B. vietnamiensis cultures reached concentrations in excess of 20 μM. We isolated a B. vietnamiensis DNA fragment containing a gene for the synthesis of decanoyl-HSL (bviI) and an open reading frame that codes for a putative signal receptor (bviR). A B. vietnamiensis bviI mutant did not produce detectable levels of decanoyl-HSL.

scholarly journals LuxR Solos in the Plant Endophyte Kosakonia sp. Strain KO348

2020 ◽  
Vol 86 (13) ◽  
Author(s):  
Susan Mosquito ◽  
Xianfa Meng ◽  
Giulia Devescovi ◽  
Iris Bertani ◽  
Alexander M. Geller ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Homoserine Lactone ◽  
The Other ◽  
The Novel ◽  
Homoserine Lactones ◽  
Interkingdom Signaling ◽  
Promoter Studies ◽  
Quorum Sensing Signals ◽  
Target Promoter ◽  
Plant Compounds

ABSTRACT Endophytes are microorganisms that live inside plants and are often beneficial for the host. Kosakonia is a novel bacterial genus that includes several species that are diazotrophic and plant associated. This study revealed two quorum sensing-related LuxR solos, designated LoxR and PsrR, in the plant endophyte Kosakonia sp. strain KO348. LoxR modeling and biochemical studies demonstrated that LoxR binds N-acyl homoserine lactones (AHLs) in a promiscuous way. PsrR, on the other hand, belongs to the subfamily of plant-associated-bacterium (PAB) LuxR solos that respond to plant compounds. Target promoter studies as well as modeling and phylogenetic comparisons suggest that PAB LuxR solos are likely to respond to different plant compounds. Finally, LoxR is involved in the regulation of T6SS and PsrR plays a role in root endosphere colonization. IMPORTANCE Cell-cell signaling in bacteria allows a synchronized and coordinated behavior of a microbial community. LuxR solos represent a subfamily of proteins in proteobacteria which most commonly detect and respond to signals produced exogenously by other microbes or eukaryotic hosts. Here, we report that a plant-beneficial bacterial endophyte belonging to the novel genus of Kosakonia possesses two LuxR solos; one is involved in the detection of exogenous N-acyl homoserine lactone quorum sensing signals and the other in detecting a compound(s) produced by the host plant. These two Kosakonia LuxR solos are therefore most likely involved in interspecies and interkingdom signaling.

scholarly journals Characterization of a Phosphotriesterase-Like Lactonase fromSulfolobus solfataricusand Its Immobilization for Disruption of Quorum Sensing

2010 ◽  
Vol 77 (4) ◽  
pp. 1181-1186 ◽  
Author(s):  
Filomena S. W. Ng ◽  
Daniel M. Wright ◽  
Stephen Y. K. Seah
Keyword(s):  
Quorum Sensing ◽  
Virulence Factors ◽  
Acyl Chain ◽  
Quorum Quenching ◽  
Homoserine Lactone ◽  
Homoserine Lactones ◽  
Specificity Constant ◽  
Chain Lengths ◽  
Quorum Sensing Signals

ABSTRACTSsoPox, a bifunctional enzyme with organophosphate hydrolase andN-acyl homoserine lactonase activities from the hyperthermophilic archaeonSulfolobus solfataricus, was overexpressed and purified from recombinantPseudomonas putidaKT2440 with a yield of 9.4 mg of protein per liter of culture. The enzyme has a preference forN-acyl homoserine lactones (AHLs) with acyl chain lengths of at least 8 carbon atoms, mainly due to lowerKmvalues for these substrates. The highest specificity constant obtained was forN-3-oxo-decanoyl homoserine lactone (kcat/Km= 5.5 × 103M−1·s−1), but SsoPox can also degradeN-butyryl homoserine lactone (C4-HSL) andN-oxo-dodecanoyl homoserine lactone (oxo-C12-HSL), which are important for quorum sensing in ourPseudomonas aeruginosamodel system. WhenP. aeruginosaPAO1 cultures were grown in the presence of SsoPox-immobilized membranes, the production of C4-HSL- and oxo-C12-HSL-regulated virulence factors, elastase, protease, and pyocyanin were significantly reduced. This is the first demonstration that immobilized quorum-quenching enzymes can be used to attenuate the production of virulence factors controlled by quorum-sensing signals.

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