Impact of exogenous gonadotropin stimulation on follicular fluid cytokine profiles

2010 ◽  
Vol 94 (4) ◽  
pp. S30
Author(s):  
E.N. Baskind ◽  
V. Sharma ◽  
N. Orsi ◽  
S. Barber
Keyword(s):  
Follicular Fluid ◽  
Cytokine Profiles ◽  
Gonadotropin Stimulation

A Case–Control Study of Follicular Fluid Cytokine Profiles in Women with Diminished Ovarian Reserve

2021 ◽  
Author(s):  
Sina Abhari ◽  
Jingqiao Lu ◽  
Heather S. Hipp ◽  
Brianne Petritis ◽  
Sabrina A. Gerkowicz ◽  
...  
Keyword(s):  
Follicular Fluid ◽  
Ovarian Reserve ◽  
Case Control Study ◽  
Case Control ◽  
Diminished Ovarian Reserve ◽  
Cytokine Profiles ◽  
Control Study

scholarly journals Impact of Exogenous Gonadotropin Stimulation on Circulatory and Follicular Fluid Cytokine Profiles

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
N. Ellissa Baskind ◽  
Nicolas M. Orsi ◽  
Vinay Sharma
Keyword(s):  
Follicular Fluid ◽  
Fluid Phase ◽  
Controlled Ovarian Hyperstimulation ◽  
Developmental Competence ◽  
Leukaemia Inhibitory Factor ◽  
Natural Cycle ◽  
Cytokine Profiles ◽  
Cytokine Levels ◽  
The Impact

Background. The natural cycle is the prototype to which we aspire to emulate in assisted reproduction techniques. Increasing evidence is emerging that controlled ovarian hyperstimulation (COH) with exogenous gonadotropins may be detrimental to oogenesis, embryo quality, and endometrial receptivity. This research aimed at assessing the impact of COH on the intrafollicular milieu by comparing follicular fluid (FF) cytokine profiles during stimulatedin vitrofertilization (IVF) and modified natural cycle (MNC) IVF.Methods. Ten women undergoing COH IVF and 10 matched women undergoing MNC IVF were recruited for this pilot study. 40 FF cytokine concentrations from individual follicles and plasma were measured by fluid-phase multiplex immunoassay. Demographic/cycle/cytokine data were compared and correlations between cytokines were computed.Results. No significant differences were found between COH and MNC groups for patient and cycle demographics, including outcome. Overall mean FF cytokine levels were higher in the MNC group for 29/40 cytokines, significantly so for leukaemia inhibitory factor and stromal cell-derived factor-1α. Furthermore, FF MNC cytokine correlations were significantly stronger than for COH data.Conclusions. These findings suggest that COH perturbs intrafollicular cytokine networks, in terms of both cytokine levels and their interrelationships. This may impact oocyte maturation/fertilization and embryo developmental competence.

Plasma and follicular fluid total renin after gonadotropin stimulation in polycystic ovarian disease

1994 ◽  
Vol 53 (2) ◽  
pp. 148
Author(s):  
I. Matinlauri ◽  
L. Anttila ◽  
P. Koskinen ◽  
M. Aalto ◽  
K. Irjala ◽  
...  
Keyword(s):  
Follicular Fluid ◽  
Polycystic Ovarian Disease ◽  
Ovarian Disease ◽  
Gonadotropin Stimulation

Evaluation of the relationship between follicular fluid oxidative stress, ovarian hormones, and response to gonadotropin stimulation

2008 ◽  
Vol 89 (4) ◽  
pp. 912-921 ◽  
Author(s):  
Malathy Appasamy ◽  
Eric Jauniaux ◽  
Paul Serhal ◽  
Ahmed Al-Qahtani ◽  
Nigel P. Groome ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Follicular Fluid ◽  
Ovarian Hormones ◽  
Gonadotropin Stimulation ◽  
The Relationship

scholarly journals Gonadotropin Stimulation Has Only a Limited Effect on the Concentration of Follicular Fluid Signalling Proteins: An Antibody Array Analysis

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Nick A. Bersinger ◽  
Markus Eisenhut ◽  
Petra Stute ◽  
Michael von Wolff
Keyword(s):  
Map Kinase ◽  
Follicular Fluid ◽  
Caspase 3 ◽  
Differentially Expressed ◽  
Matched Pair ◽  
In Vitro Fertilisation ◽  
Array Analysis ◽  
Gonadotropin Stimulation ◽  
Cystatin A ◽  
Follicular Fluids

Objective. The follicular fluid (FF) plays an essential role in the physiology of the follicle and the oocyte. Gonadotropin stimulation affects the FF steroid hormone and anti-Mullerian hormone (AMH) concentrations, which has been suggested to be the reason for lower oocyte competence in conventional gonadotropin stimulated in vitro fertilisation (cIVF) compared to natural cycle IVF (NC-IVF). To analyse the effect of gonadotropin stimulation on a broad spectrum of signalling proteins, we ran proteomic antibody arrays on FF of women undergoing both treatments NC-IVF and cIVF. Method. Twenty women underwent one NC-IVF and one cIVF treatment cycle. Follicular fluids of the first aspirated follicle were compared between the two groups using a protein microarray which included antibodies against 224 proteins related to cell signalling and reference proteins. Each of the 40 albumin-stripped, matched-pair samples was labelled in the reverse-dye (Cy3/Cy5) procedure before undergoing array hybridisation. Signal analysis was performed using normalisation algorithms in dedicated software. Five proteins yielding a value of P < 0.05 in the array experiment (Cystatin A, Caspase-3, GAD65/67, ERK-1, and ERK-2) were then submitted to quantitative determination by ELISA in the same follicular fluids. Results. Array analysis yielded only a small number of differentially expressed signalling markers by unadjusted P values. Adjustment as a consequence of multiple determinations resulted in the absence of any significant differential marker expression on the array. Five unadjusted differentially expressed proteins were quantified immunometrically with antibodies from different sources. Follicular fluid concentrations of Cystatin A and MAP kinase ERK-1 concentrations were significantly higher in the cIVF than in the NC-IVF follicles, while GAD-2 (GAD65/67) did not differ. The assays for Caspase-3 and MAP kinase ERK-2 did not have the required sensitivities. Conclusion. In contrast to FF steroid hormones and AMH, FF concentrations of signalling proteins are not or only marginally altered by gonadotropin stimulation.

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