Identifying differential expressed metabolites in viable embryos with UHPLC-ToF-MS (ultra high-performance liquid chromatography–time of flight-mass spectrometry): a comprehensive metabolomic approach

2013 ◽  
Vol 100 (3) ◽  
pp. S119
Author(s):  
Y.-C. Yang ◽  
C.-H. Kuo ◽  
Y.J. Tseng ◽  
H.-N. Ho ◽  
H.-F. Chen
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Time Of Flight ◽  
Flight Mass Spectrometry ◽  
Metabolomic Approach ◽  
Tof Ms

Determination of 4 psychoactive substances in tea using ultra high performance liquid chromatography combined with the quadrupole time-of-flight mass spectrometry

2020 ◽  
Vol 12 (40) ◽  
pp. 4878-4884
Author(s):  
Jian Li ◽  
Junmei Ma ◽  
Yan Zhang ◽  
Lei Zheng
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Time Of Flight ◽  
Psychoactive Substances ◽  
Flight Mass Spectrometry ◽  
Tof Ms

In this study, a method for the qualification and quantification of 4 psychoactive substances in tea using ultra high performance liquid chromatography coupled with the quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) has been developed.

scholarly journals Chemical Profiling of Lobelia chinensis with High-Performance Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry (HPLC/Q-TOF MS) Reveals Absence of Lobeline in the Herb

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3258 ◽  
Author(s):  
Haixing Wang ◽  
Yuanyuan Li ◽  
Yeqing Huang ◽  
Chunyan Zhao ◽  
Hon-Yeung Cheung
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Chemical Constituents ◽  
Time Of Flight ◽  
Chemical Components ◽  
Chemical Profiling ◽  
Flight Mass Spectrometry ◽  
Tof Ms

Lobelia chinensis is a kind of herbal medicine widely distributed and used in Asia. The chemical components of this herb, however, have not been well studied until now. Lobeline, as an essential and famous bioactive compound in Lobelia genus, has been assumed to be present in L. chinensis. In order to ascertain its presence and, more importantly, proper use of this herb, chemical profiling this herb with highly sensitive and high-resolution analytical mass spectrometry was applied. In this study, high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (HPLC/Q-TOF MS) method was employed to systematically profile the chemical constituents of L. chinensis for the first time. Comparative chemical profiling study of L. chinensis and Lobelia inflata was also conducted to provide evidence whether lobeline is present or not. Piperidine alkaloids except for lobeline, alkaloid-lignan hybrids, flavonoids, polyacetylenes, nonanedioic acid, and some new phytochemicals were successfully identified in L. chinensis simultaneously. Comparing to the chemical profiles of L. inflata, lobeline was found to be absent in L. chinensis. All of the secondary metabolites in L. chinensis were determined with the HPLC/Q-TOF MS method. The absence of lobeline in L. chinensis was confirmed after this extensive study.

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