Follicular fluid homocysteine levels with N-Acetyl cysteine supplemented controlled ovarian hyperstimulation, correlation with oocyte yield and ICSI cycle outcome

The first attempts at in vitro fertilization (IVF) of human oocytes were performed during cycles utilizing human menopausal gonadotropin (hMG) and human chorionic gonadotropin (hCG). These early cycles resulted in a successful conception, which unfortunately ended as a tubal gestation. The birth of Louise Brown in 1978, the first successful IVF birth, was actually achieved following fertilization during a spontaneous cycle in which ovulation was triggered with endogenous luteinizing hormone (LH).However, due to the greater margin for error afforded by larger numbers of follicles, the practice of IVF rapidly evolved towards the use of controlled ovarian hyperstimulation (COH) to achieve higher pregnancy rates. It is easy to understand why this approach evolved. Oocyte harvesting was accomplished primarily by laparoscopy. Since oocyte yield per follicle was less than 100% and fertilization rates were limited, the relatively traumatic follicle aspiration process was more likely to result in embryo transfer if a greater number of follicles was present.

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Controlled ovarian hyperstimulation induced changes in the expression of circulatory miRNA in bovine follicular fluid and blood plasma

Journal of Ovarian Research ◽

10.1186/s13048-015-0208-5 ◽

2015 ◽

Vol 8 (1) ◽

Cited By ~ 28

Author(s):

Sina Seifi Noferesti ◽

Md. Mahmodul Hasan Sohel ◽

Michael Hoelker ◽

Dessie Salilew-Wondim ◽

Ernst Tholen ◽

...

Keyword(s):

Blood Plasma ◽

Follicular Fluid ◽

Controlled Ovarian Hyperstimulation ◽

Ovarian Hyperstimulation ◽

Induced Changes

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Follicular fluid lipid peroxidation levels in women with endometriosis during controlled ovarian hyperstimulation

Human Fertility ◽

10.1080/14647273.2016.1246753 ◽

2016 ◽

Vol 20 (1) ◽

pp. 48-54 ◽

Cited By ~ 2

Author(s):

Camila Bruna de Lima ◽

Fernanda Bertuccez Cordeiro ◽

Mariana Camargo ◽

Daniel Suslik Zylbersztejn ◽

Agnaldo Pereira Cedenho ◽

...

Keyword(s):

Lipid Peroxidation ◽

Follicular Fluid ◽

Controlled Ovarian Hyperstimulation ◽

Ovarian Hyperstimulation

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Homocystein (HCY), folic acid and vitamin (B12) levels in follicular fluid (FF) and serum of patient who became pregnant and those who did not after controlled ovarian hyperstimulation (COH) for ICSI

Fertility and Sterility ◽

10.1016/j.fertnstert.2012.07.103 ◽

2012 ◽

Vol 98 (3) ◽

pp. S28

Author(s):

M.E. Hammadeh

Keyword(s):

Folic Acid ◽

Vitamin B12 ◽

Follicular Fluid ◽

Controlled Ovarian Hyperstimulation ◽

Ovarian Hyperstimulation

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Lipid peroxidation and vitamin E in serum and follicular fluid of infertile women with peritoneal endometriosis submitted to controlled ovarian hyperstimulation: a pilot study

Fertility and Sterility ◽

10.1016/j.fertnstert.2007.10.072 ◽

2008 ◽

Vol 90 (6) ◽

pp. 2080-2085 ◽

Cited By ~ 27

Author(s):

C CAMPOSPETEAN ◽

R FERRIANI ◽

R DOSREIS ◽

M DIASDEMOURA ◽

A JORDAOJR ◽

...

Keyword(s):

Lipid Peroxidation ◽

Vitamin E ◽

Pilot Study ◽

Follicular Fluid ◽

Controlled Ovarian Hyperstimulation ◽

Ovarian Hyperstimulation ◽

Infertile Women ◽

Peritoneal Endometriosis

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Ovarian modulators of 11beta-hydroxysteroid dehydrogenase (11betaHSD) activity in follicular fluid from gonadotrophin-stimulated assisted conception cycles

Reproduction ◽

10.1530/rep.0.1240801 ◽

2002 ◽

pp. 801-812 ◽

Cited By ~ 13

Author(s):

LM Thurston ◽

DP Norgate ◽

KC Jonas ◽

C Chandras ◽

HJ Kloosterboer ◽

...

Keyword(s):

Follicular Fluid ◽

Rat Kidney ◽

Hydroxysteroid Dehydrogenase ◽

Controlled Ovarian Hyperstimulation ◽

Ovarian Hyperstimulation ◽

Assisted Conception ◽

Human Follicular Fluid ◽

Glucocorticoid Metabolism ◽

Stimulation Of

In the ovary, cortisol-cortisone interconversion is catalysed by isoforms of 11beta-hydroxysteroid dehydrogenase (11betaHSD). The objective of this study was to establish whether human follicular fluid (hFF), obtained after controlled ovarian hyperstimulation, contains paracrine modulators of 11betaHSD activity. Of 274 hFF samples tested for effects in rat kidney homogenates, 206 hFF samples significantly inhibited NADP(+)-dependent oxidation of cortisol within 1 h (by 11-67% of control 11betaHSD activity), whereas 42 hFF samples significantly stimulated 11betaHSD activity (16-210% increase relative to control). Although charcoal-stripping of hFF prevented the inhibition and potentiated the stimulation of NADP(+)-dependent cortisol oxidation in a renal homogenate, effects of individual hFF samples on NADP(+)-dependent cortisol oxidation were independent of intrafollicular progesterone concentrations. Hydrophilic fractions of hFF samples, isolated by C18 column chromatography, stimulated both the NADP(+)-dependent oxidation of cortisol (by 55+/-5%, n=98) and the NADPH-dependent reduction of cortisone (by 86+/-22%, n= 5). In contrast, the hydrophobic fractions of hFF (eluted at 65-85% methanol) inhibited both NADP(+)-dependent 11beta-dehydrogenase and NADPH-dependent 11-ketosteroid reductase activities (by 63+/-2% and 74+/-4%, respectively). None of the C18 column fractions of 50 hFF samples had any significant effect on NAD(+)-dependent 11beta-dehydrogenase activities. The hydrophobic inhibitors of NADP(H)-dependent cortisol-cortisone metabolism did not co-elute with several candidate compounds (prostaglandins E(2) and F(2alpha), cortisol, cortisone, oestradiol, testosterone, progesterone, pregnenolone or cholesterol). Hence, hFF aspirated from women undergoing controlled ovarian hyperstimulation for assisted conception contains both hydrophilic stimuli and hydrophobic inhibitors of glucocorticoid metabolism which appear to be selective for the NADP(H)-dependent, type 1 isoform of 11betaHSD.

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