Regulation of conidiation, polarity growth, and pathogenicity by MrSte12 transcription factor in entomopathogenic fungus, Metarhizium rileyi

2021 ◽  
pp. 103612
Author(s):  
Yunlong Lin ◽  
Jing Wang ◽  
Kai Yang ◽  
Liqin Fan ◽  
Zhongkang Wang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Entomopathogenic Fungus ◽  
Metarhizium Rileyi

scholarly journals Analogous and Diverse Functions of APSES-Type Transcription Factors in the Morphogenesis of the Entomopathogenic Fungus Metarhizium rileyi

2020 ◽  
Vol 86 (8) ◽  
Author(s):  
Caiyan Xin ◽  
Jinping Zhang ◽  
Siji Nian ◽  
Guangxi Wang ◽  
Zhongkang Wang ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Transcriptome Analysis ◽  
Entomopathogenic Fungus ◽  
Regulatory Mechanisms ◽  
Sequencing Analysis ◽  
Fungal Virulence ◽  
Content Type ◽  
Dimorphic Transition ◽  
Metarhizium Rileyi ◽  
Fungal Morphogenesis

ABSTRACT APSES-type transcription factors (TFs) have analogous and diverse functions in the regulation of fungal morphogenesis processes. However, little is known about these functions in microsclerotium formation. In this study, we characterized two orthologous APSES genes (MrStuA and MrXbp) in the entomopathogenic fungus Metarhizium rileyi. Deletion of either MrStuA or MrXbp impaired dimorphic transition, conidiation, fungal virulence, and microsclerotium formation. Compared with the wild-type strain, ΔMrStuA and ΔMrXbp mutants were hypersensitive to thermal and oxidative stress. Furthermore, transcriptome sequencing analysis revealed that MrStuA and MrXbp independently regulate their own distinctive subsets of signaling pathways during dimorphic transition and microsclerotium formation, but they also show an overlapping regulation of genes during these two distinct morphogenesis processes. These results provide a global insight into vital roles of MrStuA and MrXbp in M. rileyi and aid in dissection of the interacting regulatory mechanisms of dimorphism transition and microsclerotium development. IMPORTANCE Transcription factors (TFs) are core components of the signaling pathway and play an important role in transcriptional regulation of gene expression during fungal morphogenesis processes. A prevailing theory suggests an interplay between different TFs regulating microsclerotial differentiation; however, the persisting issue remains that these interplay mechanisms are not clear. Here, we analyzed two members of the APSES-type TFs in Metarhizium rileyi using a gene deletion strategy and transcriptome analysis. Mutants were significantly impaired in microsclerotium formation and dimorphic transition. Transcriptome analysis provided evidence for interacting regulatory mechanisms by the two TFs in microsclerotium formation and dimorphic transition. Furthermore, we investigated their overlapping roles in mediating the expression of genes required for different fungal morphogenesis processes. Characterization of TFs in this study will aid in dissecting the interplay between regulatory mechanisms in fungal morphogenesis processes.

scholarly journals Mr-AbaA Regulates Conidiation by Interacting with the Promoter Regions of Both Mr-veA and Mr-wetA in Metarhizium robertsii

2021 ◽  
Author(s):  
Hao Wu ◽  
Youmin Tong ◽  
Rong Zhou ◽  
Yulong Wang ◽  
Zhangxun Wang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Entomopathogenic Fungus ◽  
Submerged Culture ◽  
Comprehensive Understanding ◽  
Promoter Regions ◽  
Metarhizium Robertsii ◽  
Content Type

Metarhizium robertsii is an emerging model entomopathogenic fungus for developing biopesticides; therefore, a comprehensive understanding of its conidiation is very important for its application. In this study, we revealed that the transcription factor Mr-AbaA is involved in the control of aerial conidiation and blastospore separation in submerged culture.

scholarly journals Complete mitogenome of the entomopathogenic fungus Metarhizium rileyi

2020 ◽  
Vol 5 (2) ◽  
pp. 1494-1495
Author(s):  
Shu Zhang ◽  
Li-Yuan Ren ◽  
Yong-Jie Zhang
Keyword(s):  
Entomopathogenic Fungus ◽  
Metarhizium Rileyi ◽  
Complete Mitogenome
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