Clonal relationship among Vibrio parahaemolyticus isolated from Mediterranean mussels (Mytilus galloprovincialis) and grooved carpet shells (Ruditapes decussatus) harvested in Sardinia (Italy)

2019 ◽  
Vol 84 ◽  
pp. 103258
Author(s):  
Sonia Lamon ◽  
Asmine Bastardo ◽  
Domenico Meloni ◽  
Simonetta Gianna Consolati ◽  
Federica Fois ◽  
...  
2019 ◽  
Vol 82 (11) ◽  
pp. 1851-1856 ◽  
Author(s):  
SONIA LAMON ◽  
SIMONETTA G. CONSOLATI ◽  
FEDERICA FOIS ◽  
MARIA G. CAMBULA ◽  
MARGHERITA PES ◽  
...  

ABSTRACT In this study, we investigated the occurrence, seasonal distribution, and molecular characterization of pathogenic vibrios in Mediterranean mussels (Mytilus galloprovincialis) and grooved carpet shells (Ruditapes decussatus) from two harvesting areas of Sardinia (Italy). Samples collected before and after depuration were submitted for qualitative and quantitative determination of Vibrio spp. Vibrio spp. isolates were presumptively identified by means of biochemical methods. Identification and virulence profile of Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus were performed by molecular methods. The prevalence of Vibrio spp. in M. galloprovincialis and R. decussatus was, respectively, 96 and 77%. The averaged enumeration (mean ± standard deviation) of Vibrio spp. in samples of M. galloprovincialis and R. decussatus collected at the harvesting time was 2.04 ± 0.45 and 2.51 ± 0.65 log CFU/g, respectively. The average contamination levels in samples collected after purification were 2.28 ± 0.58 log CFU/g (M. galloprovincialis) and 2.12 ± 0.67 log CFU/g (R. decussatus). Four potentially pathogenic V. parahaemolyticus isolates (tdh+ or trh+) were recovered from grooved carpet shells samples. No isolate was tdh+/trh+. The presence of potentially pathogenic vibrios in Sardinian waters strengthens the need for rational purification practices under controlled conditions to guarantee the protection of consumers.


2013 ◽  
Vol 67 (1) ◽  
pp. 36-40
Author(s):  
Carmen Lopez-Joven ◽  
Ana Roque ◽  
Josu Pérez-Larruscain ◽  
Imanol Ruiz-Zarzuela ◽  
M. Dolores Furones ◽  
...  

Author(s):  
Pedro Miguel Callapez ◽  
Ricardo Pimentel

Resumo O menir de Padrão 1 constitui um dos monumentos mais representativos do núcleo megalítico de Sagres - Vila do Bispo, localizado no extremo sudoeste do Algarve (sul de Portugal). Nas intervenções arqueológicas de que foi alvo em 1984 e 1994, sob direção de Mário Varela Gomes, foi efetuada uma amostragem exaustiva de concentrações de restos esqueléticos de invertebrados marinhos presentes num contexto de ocupação do Neolítico antigo. Considerando a sua importância para o entendimento das estratégias de subsistência, economia alimentar e práticas culturais durante este intervalo marcante da Pré-história recente, procedeu-se ao seu estudo zooarqueológico. Contabilizou-se um número mínimo de 1496 indivíduos pertencentes a 25 espécies de moluscos bivalves e gastrópodes, três de crustáceos e uma de anelídeo poliqueta. A sua composição, abundância relativa e análise paleoecológica sugerem práticas locais de subsistência, ligadas à exploração oportunista de espécies comestíveis. A recoleção seletiva incidiu em áreas rochosas expostas do litoral próximo (Patella depressa, P. ulyssiponensis, Mytilus galloprovincialis, Perna perna, Stramonita haemastoma, Pollicipes pollicipes), mas também em meio estuarino ou lagunar hoje já desaparecido (Cerastoderma edule, Ruditapes decussatus). A associação presente, com destaque para P. perna, S. haemastoma e Siphonaria pectinata, é compatível com o ótimo climático holocénico, durante o qual espécies mediterrânicas e oeste-africanas ampliaram a sua área de repartição biogeográfica para norte, até à costa portuguesa. Destaca-se, ainda, a ocorrência de P. perna, dado o seu possível carácter inédito em contextos pré-históricos portugueses. Resumen El menhir de Padrão 1 constituye uno de los monumentos más representativos del núcleo megalítico de Sagres - Vila do Bispo, ubicado en el extremo suroeste del Algarve (sur de Portugal). En las intervenciones arqueológicas de que fue objeto en 1984 y 1994, dirigidas por Mário Varela Gomes, se efectuó un muestreo exhaustivo de concentraciones de restos esqueléticos de invertebrados marinos en un contexto de ocupación del Neolítico antiguo. Considerando su importancia para el entendimiento de las estrategias de subsistencia, economía alimentaria y prácticas culturales durante este intervalo importante de la Prehistoria reciente, se procedió a su estudio zooarqueológico. Se contabilizó un número mínimo de 1496 individuos pertenecientes a 25 especies de moluscos bivalvos y gasterópodos, tres de crustáceos y una de gusano poliqueto. Su composición, abundancia relativa y análisis paleoecológico sugieren prácticas locales de subsistencia, ligadas a la explotación oportunista de especies comestibles. La recolección selectiva se centró en áreas rocosas expuestas del litoral próximo (Patella depressa, P. ulyssiponensis, Mytilus galloprovincialis, Perna perna, Stramonita haemastoma, Pollicipes pollicipes), pero también en un medio estuarino o lagunar hoy ya desaparecido (Cerastoderma edule, Ruditapes decussatus). Esta asociación, especialmente P. perna, S. haemastoma y Siphonaria pectinata, es compatible con el óptimo climático del Holoceno, durante el cual las especies del Mediterráneo y de África Occidental extendieron su área de distribución biogeográfica hacia al norte, hasta la costa portuguesa. Se destaca también la presencia de P. perna, dada su posible primera cita en contextos prehistóricos portugueses. Abstract The menhir of Padrão 1 has been known as one of the most representative monuments from the megalithic nucleus of Sagres - Vila do Bispo, in the southwest end of Algarve (South of Portugal). During two archaeological interventions made in 1984 and 1994, under direction of Mário Varela Gomes, an exhaustive sampling of concentrations of skeletal remains of marine invertebrates from an early Neolithic settlement context was made. Considering its importance to understand subsistence strategies, food economy and cultural practices during this important interval of recent Prehistory, the zooarchaeological study of this assemblage was carried out. A minimal number of 1496 individuals was counted, including 25 species of bivalve and gastropod mollusks, three of crustaceans and one of polychaete worm. Its composition, relative abundance and paleoecologic analysis suggest local strategies of subsistence based on the opportunistic exploitation of edible species. The selective recollection was more effective on intertidal rocky shores available in the proximity (Patella intermedia, P. ulyssiponensis, Mytilus galloprovincialis, Perna perna, Stramonita haemastoma, Pollicipes pollicipes), but also made from a possible estuarine or lagoonal environment already disappeared (Cerastoderma edule, Ruditapes decussatus). The faunal association, with emphasis on P. perna, S. haemastoma and Siphonaria pectinata, is compatible with the Holocene climatic optimum, when several Mediterranean and West-African species have extended their biogeographic ranges northwards, till the Portuguese coast. The mytilid P. perna also stands out, as the first occurrence of this species in Portuguese Pre-historic contexts.


2011 ◽  
Vol 59 (2) ◽  
pp. 155-164 ◽  
Author(s):  
Urška Henigman ◽  
Majda Biasizzo ◽  
Stanka Vadnjal ◽  
Andrej Kirbiš ◽  
Ivan Toplak ◽  
...  

The aim of this study was to determine the prevalence ofVibrio parahaemolyticusin shellfish samples harvested along the Slovenian coast. Shellfish samples of Mediterranean mussels (Mytilus galloprovincialis) were collected along the Slovenian coast at four locations (Seča, Piran, Strunjan and Debeli Rtič) between 2006 and 2008. Samples were examined and analysed for the presence ofV. parahaemolyticusby conventional and molecular methods. The presence ofVibrioin the samples was examined by conventional methods on plate grown bacterial cells before and after enrichment in alkaline saline peptone water (ASPW). PCR methods were used for the detection ofV. parahaemolyticus-specifictoxRandtlhgenes and of the virulence-associatedtdhandtrhgenes. Out of 168 samples examined, 24 were positive fortoxRandtlhgenes by PCR from enrichment broth. Five out of 62 (8.1%), 4 out of 32 (12.5%) and 15 out of 74 (20.2%) samples were positive in 2006, 2007 and 2008, respectively. Colonies ofV. parahaemolyticuswere isolated from only one sample positive forV. parahaemolyticusby PCR.


2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Eva Šatović ◽  
Andrea Luchetti ◽  
Juan J. Pasantes ◽  
Daniel García-Souto ◽  
Andrea Cedilak ◽  
...  

AbstractTerminal repeat retrotransposons in miniature (TRIMs) are small non-autonomous LTR retrotransposons consisting of two terminal direct repeats surrounding a short internal domain. The detection and characterization of these elements has been mainly limited to plants. Here we present the first finding of a TRIM element in bivalves, and among the first known in the kingdom Animalia. Class Bivalvia has high ecological and commercial importance in marine ecosystems and aquaculture, and, in recent years, an increasing number of genomic studies has addressed to these organisms. We have identified biv-TRIM in several bivalve species: Donax trunculus, Ruditapes decussatus, R. philippinarum, Venerupis corrugata, Polititapes rhomboides, Venus verrucosa, Dosinia exoleta, Glycymeris glycymeris, Cerastoderma edule, Magallana gigas, Mytilus galloprovincialis. biv-TRIM has several characteristics typical for this group of elements, exhibiting different variations. In addition to canonically structured elements, solo-TDRs and tandem repeats were detected. The presence of this element in the genome of each species is <1%. The phylogenetic analysis showed a complex clustering pattern of biv-TRIM elements, and indicates the involvement of horizontal transfer in the spreading of this element.


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