Characterization of cider by its hydrophobic protein profile and foam parameters

Morphology, protein profile and the level of Bedbug infestations were carried out at a University in Lagos, Nigeria after an outcry of high infestation by the students in halls of residence. Three bedbugswere each collected from nine halls of residence for morphological characterization of adults and to carry out protein profile analysis usingSodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Coomassie staining method. Level of infestation of Bedbugs was carried out in two female halls A and Busing blood smeared on the walls. All bedbugs found were Cimex hemipterus. 29.8% of the rooms in Hall Bhad no bedbug infestation, 23.9%, 20.2%, 15.4% and 10.6% had low, average, high and very high levels of bedbug infestation respectively while in A hall, it was 21.4%, 30.5%, 24.4%, 14.5% and 9.2% respectively. In A hall, 4.6%, 16.8% and 0.8% had one, two and three out of the four mattresses in the room infested with bedbugs respectively while 56.5% had all the four mattresses infested with bedbugs. In B hall, 1.6%, 16.0% and 0% had one, two and three mattresses infested with bedbugs respectively while the remaining 52.7% had all the four mattresses in the room infested with bedbugs. There was a significant relationship between the level of infestation and the number of mattresses infested in each hall (P < 0.01).The protein profile analysis of bedbugs did not show the protein bands clearly because of the low soluble protein content of Cimex hemipterus and the detection limit of Coomassie stain.

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A Comprehensive Peptidomic Approach to Characterize the Protein Profile of Selected Durum Wheat Genotypes: Implication for Coeliac Disease and Wheat Allergy

Nutrients ◽

10.3390/nu11102321 ◽

2019 ◽

Vol 11 (10) ◽

pp. 2321 ◽

Cited By ~ 4

Author(s):

Rosa Pilolli ◽

Agata Gadaleta ◽

Luigia Di Stasio ◽

Antonella Lamonaca ◽

Elisabetta De Angelis ◽

...

Keyword(s):

Risk Assessment ◽

Celiac Disease ◽

In Silico ◽

Proteolytic Enzymes ◽

Protein Profile ◽

Sequence Information ◽

Wheat Allergy ◽

Wheat Genotypes ◽

Proteomic Approach

The wheat varietal selection undertaken by breeders in recent decades has been tailored mainly to improve technological and productivity-related traits; however, the latter has resulted in a considerable impoverishment of the genetic diversity of wheat-based products available on the market. This pitfall has encouraged researchers to revalue the natural diversity of cultivated and non-cultivated wheat genotypes in light of their different toxic/immunogenic potential for celiac disease and wheat-allergic patients. In the present investigation, an advanced proteomic approach was designed for the global characterization of the protein profile of selected tetraploid wheat genotypes (Triticum turgidum). The approach combined proteins/peptides sequence information retrieved by specific enzymatic digestions (single and dual proteolytic enzymes) with protein digestibility information disclosed by means of in-vitro simulated human gastroduodenal digestion experiments. In both cases, the peptide pools were characterized by discovery analysis with liquid chromatography high-resolution tandem mass spectrometry, and specific amino acid sequences were identified via commercial software. The peptide list was screened for in silico toxicity/immunogenicity risk assessment, with the aid of various open-source bioinformatics tools for epitopes matching. Given the global information provided by the designed proteomic approach, the in silico risk assessment not only tackled toxicity implication for celiac disease patients, but also scouted for immunogenic sequences relevant for wheat allergic patients, achieving a comprehensive characterization of the protein profile of the selected genotypes. These latter were assessed to encrypt a variable number of toxic/immunogenic epitopes for celiac disease and wheat allergy, and as such they could represent convenient bases for breeding practices and for the development of new detoxification strategies.

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Characterization of urinary protein profile in regular kratom (Mitragyna speciosa korth.) users in Malaysia

Journal of Addictive Diseases ◽

10.1080/10550887.2021.1981122 ◽

2021 ◽

pp. 1-12

Author(s):

Rana Khudhair Jasim ◽

Zurina Hassan ◽

Darshan Singh ◽

Edward Boyer ◽

Lay-Harn Gam

Keyword(s):

Protein Profile ◽

Urinary Protein ◽

Mitragyna Speciosa

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Protein Profile Characterization of Hop (Humulus LupulusL.) Varieties

Journal of the American Society of Brewing Chemists ◽

10.1094/asbcj-2014-0629-01 ◽

2014 ◽

Vol 72 (3) ◽

pp. 184-191 ◽

Cited By ~ 1

Author(s):

Christoph Neugrodda ◽

Martina Gastl ◽

Thomas Becker

Keyword(s):

Protein Profile

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Protein profile characterization of bacterial lysates by capillary electrophoresis

Electrophoresis ◽

10.1002/elps.1150191311 ◽

1998 ◽

Vol 19 (13) ◽

pp. 2317-2323 ◽

Cited By ~ 22

Author(s):

Ildikó Kustos ◽

Béla Kocsis ◽

Ildikó Kerepesi ◽

Ferenc Kilár

Keyword(s):

Capillary Electrophoresis ◽

Protein Profile ◽

Bacterial Lysates

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Characterization of fractions of aqueoussoluble constituents of the mushroom Pleurotus ostreato-roseus Sing. and its effects on the serum protein profile of mice

Revista Brasileira de Farmacognosia ◽

10.1590/s0102-695x2002000300017 ◽

2002 ◽

Vol 12 ◽

pp. 36-37

Author(s):

Nerivan Barbosa de Lima ◽

Maria Raquel Querino de Sousa ◽

Onaldo Guedes Rodrigues ◽

Marcos José Correia

Keyword(s):

Serum Protein ◽

Protein Profile ◽

Serum Protein Profile

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Characterization of lactococcal bacteriophages from Quebec cheese plants

Canadian Journal of Microbiology ◽

10.1139/m92-143 ◽

1992 ◽

Vol 38 (9) ◽

pp. 875-882 ◽

Cited By ~ 79

Author(s):

Sylvain Moineau ◽

Josée Fortier ◽

Hans-W. Ackermann ◽

Sithian Pandian

Keyword(s):

Electron Microscopy ◽

Host Range ◽

Cheese Whey ◽

Protein Profile ◽

Tail Length ◽

Dna Homology ◽

Partial Loss ◽

Narrow Host Range ◽

Siphoviridae Family

This is the first study on the characterization of lactococcal phages isolated in Canada. Thirty lactococcal phages were isolated from Quebec cheese plants reporting partial loss of starter activity. Phages were characterized by electron microscopy, DNA homology, protein profile, and host range. All phages belonged to the Siphoviridae family. Seventeen phages (57%) has prolate heads (60 × 40 nm) and 100 nm long, noncontractile tails (morphotype B2, species c2). They showed strong DNA homology with other prolate-headed phages isolated from other countries (Australia, United States). In addition to normal prolate phages, most lysates contained pairs of empty heads (no DNA) connected by a small bridge. Thirteen phages (43%) had small isometric heads (55 nm in diameter) and long, noncontractile tails (morphotype B1). Based on DNA homology, 11 of these phages were found related to phage species 936 despite differences in tail length (140 to 200 nm). The two other small isometric phages, UL36 and UL39, hybridized with phage P335 DNA, and therefore belong to this species. No DNA homology was observed between prolate and small isometric phages. Phages with prolate heads showed a broader host range than small isometric-headed phages. The DNA of phage UL36, which has a relatively narrow host range, has more restriction endonuclease sites than other lactococcal bacteriophages. Key words: lactococci, bacteriophage, taxonomy, cheese, whey.

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