scholarly journals New integrative computational approaches unveil the Saccharomyces cerevisiae pheno-metabolomic fermentative profile and allow strain selection for winemaking

2016 ◽  
Vol 211 ◽  
pp. 509-520 ◽  
Author(s):  
Ricardo Franco-Duarte ◽  
Lan Umek ◽  
Inês Mendes ◽  
Cristiana C. Castro ◽  
Nuno Fonseca ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Strain Selection ◽  
Computational Approaches ◽  
Selection For

Yeast selection for non-alcoholic and low-alcoholic beverages based on wort

2019 ◽  
Vol 2 (2) ◽  
pp. 140
Author(s):  
Petar Nedyalkov ◽  
Maria Kaneva ◽  
Vesela Shopska ◽  
Rositsa Denkova ◽  
Georgi Kostov ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Strain Selection ◽  
Alcoholic Beverages ◽  
Good Growth ◽  
Saccharomyces Carlsbergensis ◽  
Yeast Selection ◽  
Yeast Strains ◽  
Selection For ◽  
Saccharomyces Diastaticus ◽  
Selection Of

A series of wort fermentations with eight yeast strains were carried out in laboratory conditions. The strains used were: Saccharomyces cerevisiae (2 strains), Saccharomyces diastaticus (3 strains), Saccharomyces carlsbergensis (1 strain), Saccharomyces lactis (1 strain), Saccharomyces sake gekkeikan (1 strain). Selection of yeast strains has been performed in order to study the possibilities for their aplication to obtain fermentable non-alcoholic and low-alcoholic beverages based on wort. Three yeast strains (two of Saccharomyces cerevisiae and one Saccharomyces diastaticus), were selected based on their good growth in the used medium and the pleasant organoleptic profile formed as a result of the fermentation carried out. The accumulated alcohol values varied between 0.05 and 0.22 % (w/w).

Yeast Strain Selection for Fuel Ethanol Production

2020 ◽  
pp. 225-243
Author(s):  
Chandra J. Panchal ◽  
Flavio Cesar Almeida Tavares
Keyword(s):  
Ethanol Production ◽  
Yeast Strain ◽  
Fuel Ethanol ◽  
Strain Selection ◽  
Selection For

scholarly journals Microhomology Selection for Microhomology Mediated End Joining in Saccharomyces cerevisiae

Genes ◽  
2019 ◽  
Vol 10 (4) ◽  
pp. 284 ◽  
Author(s):  
Kihoon Lee ◽  
Jae-Hoon Ji ◽  
Kihoon Yoon ◽  
Jun Che ◽  
Ja-Hwan Seol ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Budding Yeast ◽  
Product Formation ◽  
Dna Breaks ◽  
End Joining ◽  
Base Pairs ◽  
Circular Chromosome ◽  
Proximal Side ◽  
Selection For ◽  
Insight Into

Microhomology-mediated end joining (MMEJ) anneals short, imperfect microhomologies flanking DNA breaks, producing repair products with deletions in a Ku- and RAD52-independent fashion. Puzzlingly, MMEJ preferentially selects certain microhomologies over others, even when multiple microhomologies are available. To define rules and parameters for microhomology selection, we altered the length, the position, and the level of mismatches to the microhomologies flanking homothallic switching (HO) endonuclease-induced breaks and assessed their effect on MMEJ frequency and the types of repair product formation. We found that microhomology of eight to 20 base pairs carrying no more than 20% mismatches efficiently induced MMEJ. Deletion of MSH6 did not impact MMEJ frequency. MMEJ preferentially chose a microhomology pair that was more proximal from the break. Interestingly, MMEJ events preferentially retained the centromere proximal side of the HO break, while the sequences proximal to the telomere were frequently deleted. The asymmetry in the deletional profile among MMEJ products was reduced when HO was induced on the circular chromosome. The results provide insight into how cells search and select microhomologies for MMEJ in budding yeast.

scholarly journals Mitochondrial-nuclear coadaptation revealed through mtDNA replacements in Saccharomyces cerevisiae

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Tuc H. M. Nguyen ◽  
Sargunvir Sondhi ◽  
Andrew Ziesel ◽  
Swati Paliwal ◽  
Heather L. Fiumera
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Natural Variation ◽  
Epistatic Effect ◽  
Genetic Distances ◽  
Genetic Interactions ◽  
Effect Sizes ◽  
Ecological Niches ◽  
Environmentally Sensitive ◽  
Selection For ◽  
Growth Differences

Abstract Background Mitochondrial function requires numerous genetic interactions between mitochondrial- and nuclear- encoded genes. While selection for optimal mitonuclear interactions should result in coevolution between both genomes, evidence for mitonuclear coadaptation is challenging to document. Genetic models where mitonuclear interactions can be explored are needed. Results We systematically exchanged mtDNAs between 15 Saccharomyces cerevisiae isolates from a variety of ecological niches to create 225 unique mitochondrial-nuclear genotypes. Analysis of phenotypic profiles confirmed that environmentally-sensitive interactions between mitochondrial and nuclear genotype contributed to growth differences. Exchanges of mtDNAs between strains of the same or different clades were just as likely to demonstrate mitonuclear epistasis although epistatic effect sizes increased with genetic distances. Strains with their original mtDNAs were more fit than strains with synthetic mitonuclear combinations when grown in media that resembled isolation habitats. Conclusions This study shows that natural variation in mitonuclear interactions contributes to fitness landscapes. Multiple examples of coadapted mitochondrial-nuclear genotypes suggest that selection for mitonuclear interactions may play a role in helping yeasts adapt to novel environments and promote coevolution.

scholarly journals Strain selection for improvement ofBradyrhizobium japonicumcompetitiveness for nodulation of soybean

2008 ◽  
Vol 282 (1) ◽  
pp. 115-123 ◽  
Author(s):  
María Julia Althabegoiti ◽  
Silvina L. López-García ◽  
Carlos Piccinetti ◽  
Elías J. Mongiardini ◽  
Julieta Pérez-Giménez ◽  
...  
Keyword(s):  
Strain Selection ◽  
Selection For

scholarly journals Essential Regions of Saccharomyces cerevisiae Telomerase RNA: Separate Elements for Est1p and Est2p Interaction

2002 ◽  
Vol 22 (7) ◽  
pp. 2366-2374 ◽  
Author(s):  
April J. Livengood ◽  
Arthur J. Zaug ◽  
Thomas R. Cech
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Nucleotide Sequence ◽  
Dna Synthesis ◽  
Active Site ◽  
Telomerase Rna ◽  
Telomeric Dna ◽  
Protein Subunits ◽  
Essential Region ◽  
Selection For

ABSTRACT The Saccharomyces cerevisiae telomerase RNA subunit is encoded by the TLC1 gene. A selection for viable alleles of TLC1 RNA from a large library of random deletion alleles revealed that less than half (∼0.5 kb of the ∼1.3-kb RNA) is required for telomerase function in vivo. The main essential region (430 nucleotides), which contains the template for telomeric DNA synthesis, was required for coimmunoprecipitation with Est1p and Est2p. Furthermore, the subregion required for interaction with Est1p, the telomerase recruitment subunit, differed from those required for interaction with Est2p, the reverse transcriptase subunit. Two regions of the RNA distant from the template in the nucleotide sequence were required for Est2p binding, but the template itself was not. Having the RNA secured to the protein away from the template is proposed to facilitate the translocation of the RNA template through the active site. More generally, our results support a role for the telomerase RNA serving as a scaffold for binding key protein subunits.

scholarly journals Prolonged Maltose-Limited Cultivation of Saccharomyces cerevisiae Selects for Cells with Improved Maltose Affinity and Hypersensitivity

2004 ◽  
Vol 70 (4) ◽  
pp. 1956-1963 ◽  
Author(s):  
Mickel L. A. Jansen ◽  
Pascale Daran-Lapujade ◽  
Johannes H. de Winde ◽  
Matthew D. W. Piper ◽  
Jack T. Pronk
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Substrate Affinity ◽  
Maltose Permease ◽  
Prolonged Cultivation ◽  
Chemostat Cultures ◽  
Proton Symport ◽  
Limiting Nutrient ◽  
Substrate Saturation ◽  
Selection For ◽  
Substrate Tolerance

ABSTRACT Prolonged cultivation (>25 generations) of Saccharomyces cerevisiae in aerobic, maltose-limited chemostat cultures led to profound physiological changes. Maltose hypersensitivity was observed when cells from prolonged cultivations were suddenly exposed to excess maltose. This substrate hypersensitivity was evident from massive cell lysis and loss of viability. During prolonged cultivation at a fixed specific growth rate, the affinity for the growth-limiting nutrient (i.e., maltose) increased, as evident from a decreasing residual maltose concentration. Furthermore, the capacity of maltose-dependent proton uptake increased up to 2.5-fold during prolonged cultivation. Genome-wide transcriptome analysis showed that the increased maltose transport capacity was not primarily due to increased transcript levels of maltose-permease genes upon prolonged cultivation. We propose that selection for improved substrate affinity (ratio of maximum substrate consumption rate and substrate saturation constant) in maltose-limited cultures leads to selection for cells with an increased capacity for maltose uptake. At the same time, the accumulative nature of maltose-proton symport in S. cerevisiae leads to unrestricted uptake when maltose-adapted cells are exposed to a substrate excess. These changes were retained after isolation of individual cell lines from the chemostat cultures and nonselective cultivation, indicating that mutations were involved. The observed trade-off between substrate affinity and substrate tolerance may be relevant for metabolic engineering and strain selection for utilization of substrates that are taken up by proton symport.

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