GABA application improves the mitochondrial antioxidant system and reduces peel browning in ‘Nanguo’ pears after removal from cold storage

2019 ◽  
Vol 297 ◽  
pp. 124903 ◽  
Author(s):  
Jiaxin Li ◽  
Xin Zhou ◽  
Baodong Wei ◽  
Shunchang Cheng ◽  
Qian Zhou ◽  
...  
2018 ◽  
Vol 137 ◽  
pp. 31-37 ◽  
Author(s):  
Mesbah Babalar ◽  
Farhad Pirzad ◽  
Mohammad Ali Askari Sarcheshmeh ◽  
Alireza Talaei ◽  
Hossein Lessani

Author(s):  
Henry H. Eichelberger ◽  
John G. Baust ◽  
Robert G. Van Buskirk

For research in cell differentiation and in vitro toxicology it is essential to provide a natural state of cell structure as a benchmark for interpreting results. Hypothermosol (Cryomedical Sciences, Rockville, MD) has proven useful in insuring the viability of synthetic human epidermis during cold-storage and in maintaining the epidermis’ ability to continue to differentiate following warming.Human epidermal equivalent, EpiDerm (MatTek Corporation, Ashland, MA) consisting of fully differentiated stratified human epidermal cells were grown on a microporous membrane. EpiDerm samples were fixed before and after cold-storage (4°C) for 5 days in Hypothermosol or skin culture media (MatTek Corporation) and allowed to recover for 7 days at 37°C. EpiDerm samples were fixed 1 hour in 2.5% glutaraldehyde in sodium cacodylate buffer (pH 7.2). A secondary fixation with 0.2% ruthenium tetroxide (Polysciences, Inc., Warrington, PA) in sodium cacodylate was carried out for 3 hours at 4°C. Other samples were similarly fixed, but with 1% Osmium tetroxide in place of ruthenium tetroxide. Samples were dehydrated through a graded acetone series, infiltrated with Spurrs resin (Polysciences Inc.) and polymerized at 70°C.


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