Response surface methodology analysis towards biodegradability and antimicrobial activity of biopolymer film containing turmeric oil against Aspergillus niger

Abstract To maximize fermentable sugars production, response surface methodology (RSM) was adopted to optimize pretreatment and enzymatic hydrolysis of wheat straw powder (WSP) using the crude cellulases preparation containing xylanases from Aspergillus niger HQ-1. Factors of pretreatment including sodium hydroxide concentration, pretreatment time and temperature were found to have significant effects on sugars production. Results indicated that WSP with particle size 0.3 mm should be pretreated using 1.8% (w/v) sodium hydroxide solution with 25.0% (w/v) of solid loading at 94.0°C for 46.0 min and the optimized pretreatment conditions could result in 90.9% of cellulose recovery, 54.6% of hemicellulose recovery and 72.7% of lignin removal, respectively. Furthermore, variables of enzymatic hydrolysis including enzyme loading, biomass loading and reaction time were proved to have significant effects on sugars yields. After hydrolysis at 50°C for 44.8 h with 7.1% (w/v) of biomass loading, 8.1 FPU/g of enzyme loading and 0.2% (w/v) of Tween-80, maximum yields of reducing sugar (632.92 mg/g) and xylose (149.83 mg/g) could be obtained, respectively. In addition, holocellulose and hemicellulose conversion were 81.6% and 80.0%, respectively. To the best of our knowledge, this is the first report about systematic optimization of sodium hydroxide pretreatment and enzymatic hydrolysis of WSP using RSM.

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Optimizing the tray dryer temperature and time of white corn flour culture

Food Research ◽

10.26656/fr.2017.5(5).718 ◽

2021 ◽

Vol 5 (5) ◽

pp. 95-104

Author(s):

D. Hunaefi ◽

Rahmawati R. ◽

D. Saputra ◽

R.R. Maulani ◽

T. Muhandri

Keyword(s):

Response Surface Methodology ◽

Aspergillus Niger ◽

Water Content ◽

Response Surface ◽

Water Activity ◽

Penicillium Citrinum ◽

Drying Process ◽

Corn Flour ◽

Acremonium Strictum ◽

Candida Famata

This research aimed to optimize the tray dryer temperature and time of white corn flour culture by Response Surface Methodology (RSM). There were two cultures used in this research, namely Amylolytic Culture (AC) and Complete Culture (CC). AC consisted of Penicillium citrinum, Aspergillus niger, Acremonium strictum, and Candida famata, while CC consisted of Penicillium chrysogenum, Penicillium citrinum, Aspergillus niger, Rhizopus stolonifer, Rhizopus oryzae, Fusarium oxysporum, Acremonium strictum, Candida famata, Kodamaea ohmeri and Candida krusei/incospicua. The independent variables in this study were drying temperature and time, where the quality indicators used were total viability of mold and yeast, water content, water activity, and pH. This research used a factor response surface methodology. Data were analyzed by ANOVA with an α level of 95%. The result of this research showed that the optimum drying process for AC starter was 40°C for 10 hrs, with characteristic response viability 8.8×107 CFU/g, water activity 0.43, water content 8.90%, and pH 4.05. CC starter showed an optimum drying process at 49°C for 4.5 hrs, with characteristic response viability 4.9×107 CFU/g, water activity 0.49, water content 7.02%, and pH 3.95. The optimum tray dryer temperatures and times were achieved for AC and CC starters.

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Optimization of ultrasound extraction of functional compound from makiang seed by response surface methodology and antimicrobial activity of optimized extract with its application in orange juice

Ultrasonics Sonochemistry ◽

10.1016/j.ultsonch.2022.105916 ◽

2022 ◽

pp. 105916

Author(s):

Thitirat Sirichan ◽

Isaya Kijpatanasilp ◽

Nicha Asadatorn ◽

Kitipong Assatarakul

Keyword(s):

Antimicrobial Activity ◽

Response Surface Methodology ◽

Response Surface ◽

Orange Juice ◽

Ultrasound Extraction

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Optimization of Cellulase Production by Aspergillus niger ITBCC L74 with Bagasse as Substrate using Response Surface Methodology

HAYATI Journal of Biosciences ◽

10.4308/hjb.25.3.115 ◽

2018 ◽

Vol 25 (3) ◽

pp. 115

Author(s):

Abdullah Abdullah ◽

Hamid Hamid ◽

Marcelinus Christwardana ◽

H. Hadiyanto

Keyword(s):

Response Surface Methodology ◽

Aspergillus Niger ◽

Response Surface ◽

Cellulase Activity ◽

Cellulase Production ◽

Lignocellulosic Material ◽

Huge Amount ◽

Activity Effect ◽

Mgcl2 Concentration ◽

Important Enzyme

Cellulase is a very important enzyme for lignocelluloses based ethanol production. Bagasse contains mainly cellulose (57.76%), hemicellulose (12.44%), lignin (21.34%), and others (7.96%). Lignocellulosic material has been considered as the good option for cellulase production because it is cheap and already available in a huge amount. The objective of this research was to produce cellulase enzyme and to optimize it by using response surface methodology. The bagasse with water content of 80% was incubated with 2 ml inoculum of Aspergillus niger ITBCC L74 in a 250 ml Erlenmeyer flask. After reaching the specified time the enzyme was extracted and then determined for its activity. Effect of process parameters such as pH, urea and MgCl2 addition were studied. The optimal cellulase activity was achieved at urea concentration of 4.5% (w/w), MgCl2 concentration of 1 mM and pH of 3.5, with maximum enzyme activity was 0.630 U/gr.

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