Rheological investigations of water-soluble polysaccharides from the Tunisian brown seaweed Cystoseira compressa

The fucoidans were obtained by extraction with water and gradient precipitation with different concentrations of ethanol. The main structural characterisations and bioactivities were achieved. Four fractions of water-soluble crude fucoidans with different molecular weights – SPC60, SPC70, SPH60, SPH70 – were extracted from S. pallidum collected from the Yellow Sea, China, using cold water and hot water extraction, and fractional precipitation with gradient concentrations of ethanol. Chemical analysis demonstrated that all of these fucoidan fractions consisted of fucose, rhamnose, xylose, mannose, glucose, and galactose with different monosaccharide mole ratios. Fractions SPC60, SPC70, and SPH70 showed moderate cytotoxic activity against P388 murine leukaemia cells. Fucoidan is a kind of bioactive polysaccharide from the brown algae. This study provides a fast and feasible method to obtain fucoidans from S. pallidum, which can be used as pharmaceutical material and functional food.

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Potential value of red and brown seaweed for sustainable bioethanol production

Bangladesh Journal of Botany ◽

10.3329/bjb.v44i4.38571 ◽

2018 ◽

Vol 44 (4) ◽

pp. 565-570 ◽

Cited By ~ 5

Author(s):

Ragaa A Hamouda ◽

Mervat H Hussein ◽

Noura El-Ahmady El-Naggar

Keyword(s):

Sugar Content ◽

Brown Seaweed ◽

Biomass Pretreatment ◽

Red Seaweed ◽

Bioethanol Production ◽

Brown Seaweeds ◽

Cystoseira Compressa ◽

Dry Biomass ◽

Productive Water ◽

High Level

Algae are renewable sources of feedstock for bioethanol that can be grown on non arable lands, non productive water sources and inexpensive culture systems. Red seaweed Laurencia obtusa and brown seaweeds Cystoseira compressa, Colpomenia sinuosa were analysed by determining sugar content by HPLC and converted into suitable fermentable feedstock by NaOH, H2SO4, HCl and H3PO4 at concentrations 1, 2, 3, 4 and 5% at 21°C of 20 minutes. The efficiency of hydrolysis significantly improved by 5% HCl for Laurencia obtusa at 42.84 g sugar/100 g dry biomass. Pretreatment of Cystoseira compressa and Colpomenia sinuosa with 3 and 5% H3PO4 gave higher sugar content of 30.51 and 41.34 g/100 g dry biomass, respectively. A relatively high level ethanol of 0.146 g/g dry biomass of Laurencia obtusa was produced. Results indicate that Cystoseira compressa and Laurencia obtusa can be good feedstocks for bioethanol production.

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Effects of Drying Methods on Nitric Oxide Inhibition Potential of Water Soluble Extracts of Turbinaria turbinata: A Brown Seaweed Species of Malaysian Origin

American Journal of Drug Discovery and Development ◽

10.3923/ajdd.2013.279.285 ◽

2013 ◽

Vol 3 (4) ◽

pp. 279-285 ◽

Cited By ~ 1

Author(s):

Ademola Monsur Hammed ◽

Tawakalit Tope Asiyanbi-H ◽

Irwandi Jaswir ◽

Azura Amid ◽

Md. Zahangir Alam

Keyword(s):

Nitric Oxide ◽

Brown Seaweed ◽

Water Soluble ◽

Drying Methods ◽

Nitric Oxide Inhibition ◽

Seaweed Species ◽

Oxide Inhibition

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A Resorcinol-Formaldehyde Resin as an Embedding Material for Electron Microscopy of Membranes

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010006355x ◽

1969 ◽

Vol 27 ◽

pp. 328-329 ◽

Cited By ~ 1

Author(s):

J. G. Robertson ◽

D. F. Parsons

Keyword(s):

Electron Microscopy ◽

Osmium Tetroxide ◽

Neutral Lipids ◽

Lipid Extraction ◽

Water Soluble ◽

Formaldehyde Resin ◽

Electron Microscope Autoradiography ◽

Resorcinol Formaldehyde ◽

Major Disadvantage ◽

Cell Organization

The extraction of lipids from tissues during fixation and embedding for electron microscopy is widely recognized as a source of possible artifact, especially at the membrane level of cell organization. Lipid extraction is also a major disadvantage in electron microscope autoradiography of radioactive lipids, as in studies of the uptake of radioactive fatty acids by intestinal slices. Retention of lipids by fixation with osmium tetroxide is generally limited to glycolipids, phospholipids and highly unsaturated neutral lipids. Saturated neutral lipids and sterols tend to be easily extracted by organic dehydrating reagents prior to embedding. Retention of the more saturated lipids in embedded tissue might be achieved by developing new cross-linking reagents, by the use of highly water soluble embedding materials or by working at very low temperatures.

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Ferritin Labeled Antibody Staining of Thin Sections

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100064013 ◽

1969 ◽

Vol 27 ◽

pp. 420-421

Author(s):

J. D. McLean ◽

S. J. Singer

Keyword(s):

Biological Material ◽

Water Soluble ◽

Thin Sections ◽

Antibody Staining ◽

Thin Sectioning ◽

Ultrathin Sections

The successful application of ferritin labeled antibodies (F-A) to ultrathin sections of biological material has been hampered by two main difficulties. Firstly the normally used procedures for the preparation of material for thin sectioning often result in a loss of antigenicity. Secondly the polymers employed for embedding may non-specifically absorb the F-A. Our earlier use of cross-linked polyampholytes as embedding media partially overcame these problems. However the water-soluble monomers used for this method still extract many lipids from the material.

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The Effect of Benzene on Bluegill Olfactory Lamellae

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010011965x ◽

1985 ◽

Vol 43 ◽

pp. 574-575

Author(s):

D.R. Mattie ◽

J.W. Fisher

Keyword(s):

Surface Morphology ◽

Soluble Fraction ◽

Lepomis Macrochirus ◽

Sublethal Concentration ◽

Water Soluble ◽

Major Constituent ◽

Jet Fuels ◽

Aquatic Biota ◽

Normal Surface ◽

Cacodylate Buffer

Jet fuels such as JP-4 can be introduced into the environment and come in contact with aquatic biota in several ways. Studies in this laboratory have demonstrated JP-4 toxicity to fish. Benzene is the major constituent of the water soluble fraction of JP-4. The normal surface morphology of bluegill olfactory lamellae was examined in conjunction with electrophysiology experiments. There was no information regarding the ultrastructural and physiological responses of the olfactory epithelium of bluegills to acute benzene exposure.The purpose of this investigation was to determine the effects of benzene on the surface morphology of the nasal rosettes of the bluegill sunfish (Lepomis macrochirus). Bluegills were exposed to a sublethal concentration of 7.7±0.2ppm (+S.E.M.) benzene for five, ten or fourteen days. Nasal rosettes were fixed in 2.5% glutaraldehyde and 2.0% paraformaldehyde in 0.1M cacodylate buffer (pH 7.4) containing 1.25mM calcium chloride. Specimens were processed for scanning electron microscopy.

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