Modified Tartary buckwheat (Fagopyrum tataricum Gaertn.) starch by gaseous ozone: Structural, physicochemical and in vitro digestible properties

2021 ◽  
pp. 107365
Author(s):  
Jingwei Hu ◽  
Xiaoping Li ◽  
Zhiyuan Cheng ◽  
Xin Fan ◽  
Zhen Ma ◽  
...  
Keyword(s):  
Tartary Buckwheat ◽  
Fagopyrum Tataricum ◽  
Gaseous Ozone

Plantlet Regeneration of Tartary Buckwheat (Fagopyrum tataricum Gaertn.) in Vitro Tissue Cultures

2016 ◽  
Vol 23 (5) ◽  
pp. 468-477
Author(s):  
Cheng-Long Wang ◽  
Xue-ni Dong ◽  
Meng-qi Ding ◽  
Yi-Xiong Tang ◽  
Xue-Mei Zhu ◽  
...  
Keyword(s):  
Plantlet Regeneration ◽  
Tartary Buckwheat ◽  
Tissue Cultures ◽  
Fagopyrum Tataricum

scholarly journals Expression Analysis of Phenylpropanoid Pathway Genes and Metabolomic Analysis of Phenylpropanoid Compounds in Adventitious, Hairy, and Seedling Roots of Tartary Buckwheat

Plants ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 90
Author(s):  
Minsol Choi ◽  
Ramaraj Sathasivam ◽  
Bao Van Nguyen ◽  
Nam Il Park ◽  
Sun-Hee Woo ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Leaf Explants ◽  
Tartary Buckwheat ◽  
Pcr Analysis ◽  
Naphthaleneacetic Acid ◽  
Root Induction ◽  
Root Cultures ◽  
Fagopyrum Tataricum ◽  
Seedling Roots

Tartary buckwheat (Fagopyrum tataricum) is an important crop that belongs to the Polygonaceae family, whose roots have received considerable attention due to the presence of compounds with high nutritional and medicinal value. In this study, we aimed to develop an efficient protocol for the culture of adventitious (ARs) and hairy (HRs) roots on a half-strength Schenk and Hildebrandt (SH) medium containing different concentrations of the auxins, α-naphthaleneacetic acid (NAA), indole-3-butyric acid (IBA), and indole-3-acetic acid (IAA). The highest percentage of root induction (91.67%) was achieved with 0.5 mg/L IAA, whereas the greatest number of roots was found in 1 mg/L IAA. In contrast, 0.1 mg/L IBA returned the longest roots. As expected, HRs were obtained from in vitro leaf explants infected with Agrobacterium rhizogenes R1000. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis of 11 phenolic pathway genes revealed that five genes (FtPAL, FtC3H, FtHQT, FtCHS, and FtANS) were highly expressed in HRs, whereas only four (FtC4H, FtFLS2, FtDFR, and FtANR), and three (Ft4CL, FtCHI, and FtF3H) were recognized in the ARs and seedling roots (SRs), respectively. HPLC analysis of phenolic compounds in different root cultures showed that the majority of the phenolic compounds (both individual and total) were significantly accumulated in the HRs. Principal component analysis (PCA) identified differences among the three root types, whereby HRs were separated from ARs and SRs based on the amount of phenolic compounds present. Analysis of the metabolic pathway revealed that among the identified metabolites, the 3, 2, and 1 pathways were associated with flavonoid, flavone and flavonol, and phenylpropanoid biosynthesis, respectively. Hierarchical clustering analysis and the heat map showed that the different root cultures presented unique metabolites.

scholarly journals Callus induction of tartary buckwheat and enhancement of its flavonoids via FtCHS1 overexpression

2019 ◽  
Author(s):  
Qixin Dong ◽  
Haixia Zhao ◽  
Xuerong Zhao ◽  
Bingbing Lv ◽  
Qi Li ◽  
...  
Keyword(s):  
Metabolic Regulation ◽  
Anthocyanin Biosynthesis ◽  
Tartary Buckwheat ◽  
Ms Medium ◽  
Fagopyrum Tataricum ◽  
Traditional Health ◽  
Traditional Health Care ◽  
Genetic Transformation System ◽  
Transgenic Manipulation

Tartary buckwheat (Fagopyrum tataricum), a popular and traditional health care-related cereal, has recently been the focus of research because of its metabolic regulation of flavonoids. Elicitingtissues in vitroculture is an effective way to explore flavonoid biosynthesis mechanisms in tartary buckwheat. In the present study, we developed an in vitro genetic transformation system using the tartary buckwheat variety ‘Xiqiao No. 2’. The results showed thattherate of callus induced from hypocotylexplants on Murashige and Skoog (MS) medium containing 0.8 mg/L 6-BA and 3.5 mg/L 2,4-D was 100%. Much greater amounts of calli could then be obtained by repeated subculture on MS medium supplemented with 3.0 mg/L 6-BA and 1.0 mg/L KT. Furthermore, transgenic calli expressing the FtCHS1 gene were obtained viaAgrobacterium-mediatedtransformation. Overexpressing FtCHS1 in tartary buckwheat callus led tothe marked promotion of flavonol (P<0.01) and anthocyanin accumulation (P<0.05) due to the dramatic upregulation of the transcription of FtCHI, FtCHS2, FtFLS1, FtFLS2, FtFLS3 and FtDFR1, the genes of key enzymes involved in the flavonol and anthocyanin biosynthesis pathways (P < 0.01). This study provides solid support for further transgenic manipulation of calli as part of a system for regenerating tartary buckwheat.

In vitro Regeneration of Tartary Buckwheat (Fagopyrum tataricum Gaertn.)*

2010 ◽  
Vol 2009 (6) ◽  
pp. 786-789
Author(s):  
Chongming WU ◽  
Xinrong MA ◽  
Hong YANG ◽  
Zhibin XU ◽  
Tao WANG
Keyword(s):  
Tartary Buckwheat ◽  
Fagopyrum Tataricum

scholarly journals Callus induction of tartary buckwheat and enhancement of its flavonoids via FtCHS1 overexpression

2019 ◽  
Author(s):  
Qixin Dong ◽  
Haixia Zhao ◽  
Xuerong Zhao ◽  
Bingbing Lv ◽  
Qi Li ◽  
...  
Keyword(s):  
Metabolic Regulation ◽  
Anthocyanin Biosynthesis ◽  
Tartary Buckwheat ◽  
Ms Medium ◽  
Fagopyrum Tataricum ◽  
Traditional Health ◽  
Traditional Health Care ◽  
Genetic Transformation System ◽  
Transgenic Manipulation

Tartary buckwheat (Fagopyrum tataricum), a popular and traditional health care-related cereal, has recently been the focus of research because of its metabolic regulation of flavonoids. Elicitingtissues in vitroculture is an effective way to explore flavonoid biosynthesis mechanisms in tartary buckwheat. In the present study, we developed an in vitro genetic transformation system using the tartary buckwheat variety ‘Xiqiao No. 2’. The results showed thattherate of callus induced from hypocotylexplants on Murashige and Skoog (MS) medium containing 0.8 mg/L 6-BA and 3.5 mg/L 2,4-D was 100%. Much greater amounts of calli could then be obtained by repeated subculture on MS medium supplemented with 3.0 mg/L 6-BA and 1.0 mg/L KT. Furthermore, transgenic calli expressing the FtCHS1 gene were obtained viaAgrobacterium-mediatedtransformation. Overexpressing FtCHS1 in tartary buckwheat callus led tothe marked promotion of flavonol (P<0.01) and anthocyanin accumulation (P<0.05) due to the dramatic upregulation of the transcription of FtCHI, FtCHS2, FtFLS1, FtFLS2, FtFLS3 and FtDFR1, the genes of key enzymes involved in the flavonol and anthocyanin biosynthesis pathways (P < 0.01). This study provides solid support for further transgenic manipulation of calli as part of a system for regenerating tartary buckwheat.

scholarly journals Micropropagation of Tartary Buckwheat (Fagopyrum tataricum Gaertn.) Plants In Vitro

2007 ◽  
Vol 45 (2) ◽  
pp. 127-132
Author(s):  
Wanna MANGKITA ◽  
Ornpapa ANUGOOLPRASERT ◽  
Ryo OHSAWA ◽  
Shigeru HISAJIMA
Keyword(s):  
Tartary Buckwheat ◽  
Fagopyrum Tataricum

Development of Rutin-rich Noodles Using Trace-rutinosidase Variety of Tartary Buckwheat (Fagopyrum Tataricum Gaertn.) ‘Manten-Kirari’

2019 ◽  
Vol 25 (6) ◽  
pp. 915-920
Author(s):  
Tatsuro Suzuki ◽  
Toshikazu Morishita ◽  
Shigenobu Takigawa ◽  
Takahiro Noda ◽  
Koji Ishiguro
Keyword(s):  
Tartary Buckwheat ◽  
Fagopyrum Tataricum

scholarly journals Effect of Gaseous Ozone on Listeria monocytogenes Planktonic Cells and Biofilm: An In Vitro Study

Foods ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1484
Author(s):  
Felice Panebianco ◽  
Selene Rubiola ◽  
Francesco Chiesa ◽  
Tiziana Civera ◽  
Pierluigi Aldo Di Ciccio
Keyword(s):  
Listeria Monocytogenes ◽  
In Vitro Study ◽  
Planktonic Cells ◽  
Free Living ◽  
Biofilm Inhibition ◽  
Additional Tool ◽  
Complete Inactivation ◽  
Gaseous Ozone ◽  
Food Borne

Among food-borne pathogens, Listeria monocytogenes continues to pose concerns to food business operators due to its capacity to form biofilm in processing environments. Ozone may be an eco-friendly technology to control microbial contaminations, but data concerning its effect on Listeria monocytogenes biofilm are still limited. In this study, the effect of gaseous ozone at 50 ppm on planktonic cells and biofilm of reference and food-related Listeria monocytogenes strains was evaluated. Ozone caused a reduction in microbial loads of 3.7 ± 0.4 and 3.9 ± 0.4 Log10 CFU/mL after 10 and 30 min, respectively. A complete inactivation of planktonic cells after 6 h of treatment was observed. Biofilm inhibition and eradication treatments (50 ppm, 6 h) resulted in a significant decrease of the biofilm biomass for 59% of the strains tested, whilst a slight dampening of live cell loads in the biofilm state was observed. In conclusion, gaseous ozone is not sufficient to completely counteract Listeria monocytogenes biofilm, but it may be useful as an additional tool to contrast Listeria monocytogenes free-living cells and to improve the existing sanitization procedures in food processing environments.

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