scholarly journals Callus induction of tartary buckwheat and enhancement of its flavonoids via FtCHS1 overexpression

2019 ◽  
Author(s):  
Qixin Dong ◽  
Haixia Zhao ◽  
Xuerong Zhao ◽  
Bingbing Lv ◽  
Qi Li ◽  
...  
Keyword(s):  
Metabolic Regulation ◽  
Anthocyanin Biosynthesis ◽  
Tartary Buckwheat ◽  
Ms Medium ◽  
Fagopyrum Tataricum ◽  
Traditional Health ◽  
Traditional Health Care ◽  
Genetic Transformation System ◽  
Transgenic Manipulation

Tartary buckwheat (Fagopyrum tataricum), a popular and traditional health care-related cereal, has recently been the focus of research because of its metabolic regulation of flavonoids. Elicitingtissues in vitroculture is an effective way to explore flavonoid biosynthesis mechanisms in tartary buckwheat. In the present study, we developed an in vitro genetic transformation system using the tartary buckwheat variety ‘Xiqiao No. 2’. The results showed thattherate of callus induced from hypocotylexplants on Murashige and Skoog (MS) medium containing 0.8 mg/L 6-BA and 3.5 mg/L 2,4-D was 100%. Much greater amounts of calli could then be obtained by repeated subculture on MS medium supplemented with 3.0 mg/L 6-BA and 1.0 mg/L KT. Furthermore, transgenic calli expressing the FtCHS1 gene were obtained viaAgrobacterium-mediatedtransformation. Overexpressing FtCHS1 in tartary buckwheat callus led tothe marked promotion of flavonol (P<0.01) and anthocyanin accumulation (P<0.05) due to the dramatic upregulation of the transcription of FtCHI, FtCHS2, FtFLS1, FtFLS2, FtFLS3 and FtDFR1, the genes of key enzymes involved in the flavonol and anthocyanin biosynthesis pathways (P < 0.01). This study provides solid support for further transgenic manipulation of calli as part of a system for regenerating tartary buckwheat.

scholarly journals Callus induction of tartary buckwheat and enhancement of its flavonoids via FtCHS1 overexpression

2019 ◽  
Author(s):  
Qixin Dong ◽  
Haixia Zhao ◽  
Xuerong Zhao ◽  
Bingbing Lv ◽  
Qi Li ◽  
...  
Keyword(s):  
Metabolic Regulation ◽  
Anthocyanin Biosynthesis ◽  
Tartary Buckwheat ◽  
Ms Medium ◽  
Fagopyrum Tataricum ◽  
Traditional Health ◽  
Traditional Health Care ◽  
Genetic Transformation System ◽  
Transgenic Manipulation

Tartary buckwheat (Fagopyrum tataricum), a popular and traditional health care-related cereal, has recently been the focus of research because of its metabolic regulation of flavonoids. Elicitingtissues in vitroculture is an effective way to explore flavonoid biosynthesis mechanisms in tartary buckwheat. In the present study, we developed an in vitro genetic transformation system using the tartary buckwheat variety ‘Xiqiao No. 2’. The results showed thattherate of callus induced from hypocotylexplants on Murashige and Skoog (MS) medium containing 0.8 mg/L 6-BA and 3.5 mg/L 2,4-D was 100%. Much greater amounts of calli could then be obtained by repeated subculture on MS medium supplemented with 3.0 mg/L 6-BA and 1.0 mg/L KT. Furthermore, transgenic calli expressing the FtCHS1 gene were obtained viaAgrobacterium-mediatedtransformation. Overexpressing FtCHS1 in tartary buckwheat callus led tothe marked promotion of flavonol (P<0.01) and anthocyanin accumulation (P<0.05) due to the dramatic upregulation of the transcription of FtCHI, FtCHS2, FtFLS1, FtFLS2, FtFLS3 and FtDFR1, the genes of key enzymes involved in the flavonol and anthocyanin biosynthesis pathways (P < 0.01). This study provides solid support for further transgenic manipulation of calli as part of a system for regenerating tartary buckwheat.

scholarly journals Genome-wide analysis of BBX gene family in Tartary buckwheat (Fagopyrum tataricum)

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11939
Author(s):  
Jiali Zhao ◽  
Hongyou Li ◽  
Juan Huang ◽  
Taoxiong Shi ◽  
Ziye Meng ◽  
...  
Keyword(s):  
Growth And Development ◽  
Environmental Changes ◽  
Anthocyanin Biosynthesis ◽  
Expression Patterns ◽  
Tartary Buckwheat ◽  
Chromosome 6 ◽  
Fagopyrum Tataricum ◽  
Conserved Motifs ◽  
Genome Wide ◽  
Gene Structures

BBX (B-box), a zinc finger transcription factor with one or two B-box domains, plays an important role in plant photomorphogenesis, growth, and development as well as response to environmental changes. In this study, 28 Tartary buckwheat BBX (FtBBX) genes were identified and screened using a comparison program. Their physicochemical properties, gene structures, conserved motifs, distribution in chromosomal, and phylogeny of the coding proteins, as well as their expression patterns, were analyzed. In addition, multiple collinearity analysis in three monocots and three dicot species illustrated that the BBX proteins identified from monocots clustered separately from those of dicots. Moreover, the expression of 11 candidate BBX genes with probable involvement in the regulation of anthocyanin biosynthesis was analyzed in the sprouts of Tartary buckwheat during light treatment. The results of gene structure analysis showed that all the 28 BBX genes contained B-box domain, three genes lacked introns, and these genes were unevenly distributed on the other seven chromosomes except for chromosome 6. The 28 proteins contained 10 conserved motifs and could be divided into five subfamilies. BBX genes of Tartary buckwheat showed varying expression under different conditions demonstrating that FtBBXs might play important roles in Tartary buckwheat growth and development. This study lays a foundation for further understanding of Tartary buckwheat BBX genes and their functions in growth and development as well as regulation of pigmentation in Tartary buckwheat.

Plantlet Regeneration of Tartary Buckwheat (Fagopyrum tataricum Gaertn.) in Vitro Tissue Cultures

2016 ◽  
Vol 23 (5) ◽  
pp. 468-477
Author(s):  
Cheng-Long Wang ◽  
Xue-ni Dong ◽  
Meng-qi Ding ◽  
Yi-Xiong Tang ◽  
Xue-Mei Zhu ◽  
...  
Keyword(s):  
Plantlet Regeneration ◽  
Tartary Buckwheat ◽  
Tissue Cultures ◽  
Fagopyrum Tataricum

scholarly journals Expression Analysis of Phenylpropanoid Pathway Genes and Metabolomic Analysis of Phenylpropanoid Compounds in Adventitious, Hairy, and Seedling Roots of Tartary Buckwheat

Plants ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 90
Author(s):  
Minsol Choi ◽  
Ramaraj Sathasivam ◽  
Bao Van Nguyen ◽  
Nam Il Park ◽  
Sun-Hee Woo ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Leaf Explants ◽  
Tartary Buckwheat ◽  
Pcr Analysis ◽  
Naphthaleneacetic Acid ◽  
Root Induction ◽  
Root Cultures ◽  
Fagopyrum Tataricum ◽  
Seedling Roots

Tartary buckwheat (Fagopyrum tataricum) is an important crop that belongs to the Polygonaceae family, whose roots have received considerable attention due to the presence of compounds with high nutritional and medicinal value. In this study, we aimed to develop an efficient protocol for the culture of adventitious (ARs) and hairy (HRs) roots on a half-strength Schenk and Hildebrandt (SH) medium containing different concentrations of the auxins, α-naphthaleneacetic acid (NAA), indole-3-butyric acid (IBA), and indole-3-acetic acid (IAA). The highest percentage of root induction (91.67%) was achieved with 0.5 mg/L IAA, whereas the greatest number of roots was found in 1 mg/L IAA. In contrast, 0.1 mg/L IBA returned the longest roots. As expected, HRs were obtained from in vitro leaf explants infected with Agrobacterium rhizogenes R1000. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis of 11 phenolic pathway genes revealed that five genes (FtPAL, FtC3H, FtHQT, FtCHS, and FtANS) were highly expressed in HRs, whereas only four (FtC4H, FtFLS2, FtDFR, and FtANR), and three (Ft4CL, FtCHI, and FtF3H) were recognized in the ARs and seedling roots (SRs), respectively. HPLC analysis of phenolic compounds in different root cultures showed that the majority of the phenolic compounds (both individual and total) were significantly accumulated in the HRs. Principal component analysis (PCA) identified differences among the three root types, whereby HRs were separated from ARs and SRs based on the amount of phenolic compounds present. Analysis of the metabolic pathway revealed that among the identified metabolites, the 3, 2, and 1 pathways were associated with flavonoid, flavone and flavonol, and phenylpropanoid biosynthesis, respectively. Hierarchical clustering analysis and the heat map showed that the different root cultures presented unique metabolites.

In vitro Regeneration of Tartary Buckwheat (Fagopyrum tataricum Gaertn.)*

2010 ◽  
Vol 2009 (6) ◽  
pp. 786-789
Author(s):  
Chongming WU ◽  
Xinrong MA ◽  
Hong YANG ◽  
Zhibin XU ◽  
Tao WANG
Keyword(s):  
Tartary Buckwheat ◽  
Fagopyrum Tataricum

scholarly journals Micropropagation of Tartary Buckwheat (Fagopyrum tataricum Gaertn.) Plants In Vitro

2007 ◽  
Vol 45 (2) ◽  
pp. 127-132
Author(s):  
Wanna MANGKITA ◽  
Ornpapa ANUGOOLPRASERT ◽  
Ryo OHSAWA ◽  
Shigeru HISAJIMA
Keyword(s):  
Tartary Buckwheat ◽  
Fagopyrum Tataricum

MICROPROPAGATION OF CHICKPEA FROM SHOOT TIP AND NODE SEGMENT

2018 ◽  
Vol 24 (2) ◽  
Author(s):  
J. D. BARSHILE
Keyword(s):  
Shoot Tip ◽  
Root Induction ◽  
Multiple Response ◽  
Ms Medium ◽  
Growth Responses ◽  
Rapid Multiplication ◽  
Micro Propagation ◽  
G 12 ◽  
Better Than

Present investigation was undertaken to standardize technique for in vitro micro-propagation of chickpea( Cicer arietinum ) cultivar Vishwas (Phule G 12). Micropropagation method for chickpea was established and this method enabled much more efficient propagation of plants. The present work was aimed at evolving a protocol for rapid multiplication of chickpea using micropropagation technique. Explants from shoot tip and node segment were cultured on MS medium supplemented with different concentrations of BAP and Kinetin (1.0 to 2.5 mg/l) and their growth responses like shooting were elucidated. The maximum multiple response was observed with 2 mg/l concentration of BAP from both types of explant. The highest number of shoots (12.5 ± 0.3) was achieved on MS medium with 2 mg/l BAP using node segments. The medium supplemented with 2 mg/l of BAP was found better than all other concentrations. Individual shoots were transferred to IBA and IAA (1.0-1.5 mg/l) for root induction. MS medium supplemented with 2 mg/l of IBA proved better for rooting. Rooted plantlets were successfully hardened in greenhouse and established in the pot.

Callus Induction and Plant Regeneration in Rauvolfia Serpentina (L.) Benth Ex. Kurz.

2009 ◽  
Vol 24 ◽  
pp. 82-88 ◽  
Author(s):  
Saraswoti Aryal ◽  
Sanu Devi Joshi
Keyword(s):  
Acetic Acid ◽  
Callus Induction ◽  
Coconut Milk ◽  
Ms Medium ◽  
Rauvolfia Serpentina ◽  
History Museum ◽  
Short Period ◽  
Murashige Skoog ◽  
Callus Tissue Culture

Rauvolfia serpentina (L.) ex. Kurz is an important medicinal plant. Callus induction and regeneration was studied from stem explant of in-vitro grown plant of Rauvolfia serpentina(L.) Benth. ex Kurz (Apocynaceae) on Murashige Skoog (1962) medium supplemented with 1mg/l 2,4-Dichlorophenocy acetic acid (2,4-D) and 1mg/l Kinetin (Kn). Vigorous growth of callus occurs after 4 weeks of culture. Callus was sub-cultured on Murashige and Skoog (MS) medium supplemented with different concentration of 2, 4-D (0.5-3.0 mg/l) and 10% coconut milk. Regeneration of plantlets occurred on MS medium containing 3 mg/1 of 2, 4-D and 10% coconut milk. These plantlets were rooted on MS medium supplemented with 1 mg/l IAA .The regenerated plantlets were able to grow on soil after short period ofacclimatization. Key words: Explant; In-vitro culture; MS medium;  2, 4 Dichlorophenoxy acetic acid; Kinetin; Callus; Tissue culture; Coconut milk. Journal of Natural History Museum Vol. 24, 2009 Page: 82-88

Development of Rutin-rich Noodles Using Trace-rutinosidase Variety of Tartary Buckwheat (Fagopyrum Tataricum Gaertn.) ‘Manten-Kirari’

2019 ◽  
Vol 25 (6) ◽  
pp. 915-920
Author(s):  
Tatsuro Suzuki ◽  
Toshikazu Morishita ◽  
Shigenobu Takigawa ◽  
Takahiro Noda ◽  
Koji Ishiguro
Keyword(s):  
Tartary Buckwheat ◽  
Fagopyrum Tataricum
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