The fine structure of ash cambium was studied after glutaraldehyde-osmium tetroxide fixation. The fusiform and ray initials are essentially alike, and both have the basic complement of organelles and membranes typical of parenchyma cells. The varied behavior of the two types of initials and the role of cambium in oriented production of the xylem and phloem are still unexplained phenomena. Actively growing cambial cells are highly vacuolate. They are rich in endoplasmic reticulum of the rough cisternal form, ribosomes, dictyosomes, and coated vesicles. Microtubules are present in the peripheral cytoplasm. The plasmalemma appears to be continuous with the endoplasmic reticulum and produces coated vesicles as well as micropinocytotic vesicles with smooth surfaces. The plastids have varying amounts of an intralamellar inclusion which may be a lipoprotein. The quiescent cambium is deficient in rough ER and coated vesicles and has certain structures which may be condensed proteins.
Optimizing Conservation Strategies for a Threatened Tree Species: In Situ Conservation of White Ash (Fraxinus americana L.) Genetic Diversity through Insecticide Treatment