In vitro effects of Italian Lavandula multifida L. leaf extracts on gilthead seabream ( Sparus aurata ) leucocytes and SAF-1 cells

2017 ◽  
Vol 66 ◽  
pp. 334-344 ◽  
Author(s):  
Angela Fazio ◽  
Rebeca Cerezuela ◽  
Maria Rosaria Panuccio ◽  
Alberto Cuesta ◽  
Maria Ángeles Esteban
Keyword(s):  
Sparus Aurata ◽  
Gilthead Seabream ◽  
Leaf Extracts ◽  
In Vitro Effects

Toxicological in vitro effects of heavy metals on gilthead seabream (Sparus aurata L.) head–kidney leucocytes

2015 ◽  
Vol 30 (1) ◽  
pp. 412-420 ◽  
Author(s):  
Patricia Morcillo ◽  
Héctor Cordero ◽  
José Meseguer ◽  
María Á. Esteban ◽  
Alberto Cuesta
Keyword(s):  
Heavy Metals ◽  
Sparus Aurata ◽  
Head Kidney ◽  
Gilthead Seabream ◽  
In Vitro Effects

scholarly journals Functionalized Nanoplastics (NPs) Increase the Toxicity of Metals in Fish Cell Lines

2021 ◽  
Vol 22 (13) ◽  
pp. 7141
Author(s):  
Carmen González-Fernández ◽  
Francisco Guillermo Díaz Baños ◽  
María Ángeles Esteban ◽  
Alberto Cuesta
Keyword(s):  
Sparus Aurata ◽  
Gilthead Seabream ◽  
Fish Cell ◽  
Test Model ◽  
Fish Cell Lines ◽  
Polystyrene Nanoparticles ◽  
Commercial Fish ◽  
Cellular Protection ◽  
Marine Life

Nanoplastics (NPs) are one of the most abundant environment-threatening nanomaterials on the market. The objective of this study was to determine in vitro if functionalized NPs are cytotoxic by themselves or increase the toxicity of metals. For that, we used 50 nm polystyrene nanoparticles with distinct surface functionalization (pristine, PS-Plain; carboxylic, PS-COOH; and amino PS-NH2) alone or combined with the metals arsenic (As) and methylmercury (MeHg), which possess an environmental risk to marine life. As test model, we chose a brain-derived cell line (SaB-1) from gilthead seabream (Sparus aurata), one of the most commercial fish species in the Mediterranean. First, only the PS-NH2 NPs were toxic to SaB-1 cells. NPs seem to be internalized into the cells but they showed little alteration in the transcription of genes related to oxidative stress (nrf2, cat, gr, gsta), cellular protection against metals (mta) or apoptosis (bcl2, bax). However, NPs, mainly PS-COOH and PS-NH2, significantly increased the toxicity of both metals. Since the coexistence of NPs and other pollutants in the aquatic environment is inevitable, our results reveal that the combined effect of NPs with the rest of pollutants deserves more attention.

scholarly journals Insights into the Function and Evolution of Taste 1 Receptor Gene Family in the Carnivore Fish Gilthead Seabream (Sparus aurata)

2020 ◽  
Vol 21 (20) ◽  
pp. 7732
Author(s):  
Anna Rita Angotzi ◽  
Sara Puchol ◽  
Jose M. Cerdá-Reverter ◽  
Sofia Morais
Keyword(s):  
Gene Family ◽  
Sparus Aurata ◽  
Functional Divergence ◽  
Receptor Gene ◽  
Feeding Strategies ◽  
Gilthead Seabream ◽  
Stable Gene ◽  
Gene Repertoire ◽  
Reporter System

A plethora of molecular and functional studies in tetrapods has led to the discovery of multiple taste 1 receptor (T1R) genes encoding G-protein coupled receptors (GPCRs) responsible for sweet (T1R2 + T1R3) and umami (T1R1 + T1R3) taste. In fish, the T1R gene family repertoires greatly expanded because of several T1R2 gene duplications, and recent studies have shown T1R2 functional divergence from canonical mammalian sweet taste perceptions, putatively as an adaptive mechanism to develop distinct feeding strategies in highly diverse aquatic habitats. We addressed this question in the carnivore fish gilthead seabream (Sparus aurata), a model species of aquaculture interest, and found that the saT1R gene repertoire consists of eight members including saT1R1, saT1R3 and six saT1R2a-f gene duplicates, adding further evidence to the evolutionary complexity of fishT1Rs families. To analyze saT1R taste functions, we first developed a stable gene reporter system based on Ca2+-dependent calcineurin/NFAT signaling to examine specifically in vitro the responses of a subset of saT1R heterodimers to L-amino acids (L-AAs) and sweet ligands. We show that although differentially tuned in sensitivity and magnitude of responses, saT1R1/R3, saT1R2a/R3 and saT1R2b/R3 may equally serve to transduce amino acid taste sensations. Furthermore, we present preliminary information on the potential involvement of the Gi protein alpha subunits saGαi1 and saGαi2 in taste signal transduction.

17α-Ethynylestradiol alters the immune response of the teleost gilthead seabream (Sparus aurata L.) both in vivo and in vitro

2012 ◽  
Vol 36 (3) ◽  
pp. 547-556 ◽  
Author(s):  
Isabel Cabas ◽  
Sergio Liarte ◽  
Alicia García-Alcázar ◽  
José Meseguer ◽  
Victoriano Mulero ◽  
...  
Keyword(s):  
Immune Response ◽  
Sparus Aurata ◽  
Gilthead Seabream
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