Expression of a highly differentiated phenotype and hepatic functionality markers in gilthead seabream (Sparus aurata L.) long-cultured hepatocytes: first morphological and functional in vitro characterization

2010 ◽  
Vol 21 (3) ◽  
pp. 571-590 ◽  
Author(s):  
Maria Pia Santacroce ◽  
Valentina Zacchino ◽  
Elisabetta Casalino ◽  
Elisabetta Merra ◽  
Alessandra Tateo ◽  
...  
Keyword(s):  
Sparus Aurata ◽  
Gilthead Seabream ◽  
Cultured Hepatocytes ◽  
In Vitro Characterization

scholarly journals Functionalized Nanoplastics (NPs) Increase the Toxicity of Metals in Fish Cell Lines

2021 ◽  
Vol 22 (13) ◽  
pp. 7141
Author(s):  
Carmen González-Fernández ◽  
Francisco Guillermo Díaz Baños ◽  
María Ángeles Esteban ◽  
Alberto Cuesta
Keyword(s):  
Sparus Aurata ◽  
Gilthead Seabream ◽  
Fish Cell ◽  
Test Model ◽  
Fish Cell Lines ◽  
Polystyrene Nanoparticles ◽  
Commercial Fish ◽  
Cellular Protection ◽  
Marine Life

Nanoplastics (NPs) are one of the most abundant environment-threatening nanomaterials on the market. The objective of this study was to determine in vitro if functionalized NPs are cytotoxic by themselves or increase the toxicity of metals. For that, we used 50 nm polystyrene nanoparticles with distinct surface functionalization (pristine, PS-Plain; carboxylic, PS-COOH; and amino PS-NH2) alone or combined with the metals arsenic (As) and methylmercury (MeHg), which possess an environmental risk to marine life. As test model, we chose a brain-derived cell line (SaB-1) from gilthead seabream (Sparus aurata), one of the most commercial fish species in the Mediterranean. First, only the PS-NH2 NPs were toxic to SaB-1 cells. NPs seem to be internalized into the cells but they showed little alteration in the transcription of genes related to oxidative stress (nrf2, cat, gr, gsta), cellular protection against metals (mta) or apoptosis (bcl2, bax). However, NPs, mainly PS-COOH and PS-NH2, significantly increased the toxicity of both metals. Since the coexistence of NPs and other pollutants in the aquatic environment is inevitable, our results reveal that the combined effect of NPs with the rest of pollutants deserves more attention.

In vitro effects of Italian Lavandula multifida L. leaf extracts on gilthead seabream ( Sparus aurata ) leucocytes and SAF-1 cells

2017 ◽  
Vol 66 ◽  
pp. 334-344 ◽  
Author(s):  
Angela Fazio ◽  
Rebeca Cerezuela ◽  
Maria Rosaria Panuccio ◽  
Alberto Cuesta ◽  
Maria Ángeles Esteban
Keyword(s):  
Sparus Aurata ◽  
Gilthead Seabream ◽  
Leaf Extracts ◽  
In Vitro Effects

scholarly journals Insights into the Function and Evolution of Taste 1 Receptor Gene Family in the Carnivore Fish Gilthead Seabream (Sparus aurata)

2020 ◽  
Vol 21 (20) ◽  
pp. 7732
Author(s):  
Anna Rita Angotzi ◽  
Sara Puchol ◽  
Jose M. Cerdá-Reverter ◽  
Sofia Morais
Keyword(s):  
Gene Family ◽  
Sparus Aurata ◽  
Functional Divergence ◽  
Receptor Gene ◽  
Feeding Strategies ◽  
Gilthead Seabream ◽  
Stable Gene ◽  
Gene Repertoire ◽  
Reporter System

A plethora of molecular and functional studies in tetrapods has led to the discovery of multiple taste 1 receptor (T1R) genes encoding G-protein coupled receptors (GPCRs) responsible for sweet (T1R2 + T1R3) and umami (T1R1 + T1R3) taste. In fish, the T1R gene family repertoires greatly expanded because of several T1R2 gene duplications, and recent studies have shown T1R2 functional divergence from canonical mammalian sweet taste perceptions, putatively as an adaptive mechanism to develop distinct feeding strategies in highly diverse aquatic habitats. We addressed this question in the carnivore fish gilthead seabream (Sparus aurata), a model species of aquaculture interest, and found that the saT1R gene repertoire consists of eight members including saT1R1, saT1R3 and six saT1R2a-f gene duplicates, adding further evidence to the evolutionary complexity of fishT1Rs families. To analyze saT1R taste functions, we first developed a stable gene reporter system based on Ca2+-dependent calcineurin/NFAT signaling to examine specifically in vitro the responses of a subset of saT1R heterodimers to L-amino acids (L-AAs) and sweet ligands. We show that although differentially tuned in sensitivity and magnitude of responses, saT1R1/R3, saT1R2a/R3 and saT1R2b/R3 may equally serve to transduce amino acid taste sensations. Furthermore, we present preliminary information on the potential involvement of the Gi protein alpha subunits saGαi1 and saGαi2 in taste signal transduction.

17α-Ethynylestradiol alters the immune response of the teleost gilthead seabream (Sparus aurata L.) both in vivo and in vitro

2012 ◽  
Vol 36 (3) ◽  
pp. 547-556 ◽  
Author(s):  
Isabel Cabas ◽  
Sergio Liarte ◽  
Alicia García-Alcázar ◽  
José Meseguer ◽  
Victoriano Mulero ◽  
...  
Keyword(s):  
Immune Response ◽  
Sparus Aurata ◽  
Gilthead Seabream

scholarly journals Towards a deeper understanding of fatty acid bioaccessibility and its dependence on culinary treatment and lipid class: a case study of gilthead seabream (Sparus aurata)

2016 ◽  
Vol 116 (10) ◽  
pp. 1816-1823 ◽  
Author(s):  
Sara Costa ◽  
Cláudia Afonso ◽  
Carlos Cardoso ◽  
Rui Oliveira ◽  
Francisca Alves ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Lipid Class ◽  
Sparus Aurata ◽  
Gilthead Seabream ◽  
Total Lipids ◽  
Class A ◽  
The Impact ◽  
Long Chain

AbstractThe bioaccessibility of total lipids and fatty acids (FA) in raw and grilled gilthead seabream (Sparus aurata) was determined using an in vitro digestion model. The particular impact of grilling on the FA profile of seabream was also studied. In addition, the influence of lipid class on the bioaccessibility of each FA was analysed. Grilling did not change the relative FA profile, and only the absolute values were altered. However, the relative FA profile varied across lipid classes, being more dissimilar between TAG and phospholipids. Long-chain SFA and PUFA seemed to be less bioaccessible. Moreover, grilling reduced bioaccessibility of protein, fat and many FA, with the highest reductions found in PUFA such as the DHA. Strong evidence supporting a predominantly regioselective action of lipase during in vitro digestion was found, and the impact of this phenomenon on FA bioaccessibility was assessed.

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