Six genes of ompA family shuffling for development of polyvalent vaccines against Vibrio alginolyticus and Edwardsiella tarda

Background. This study describes antimicrobial, anticancer and antioxidant properties of Sauropus androgynus stem extract. The main objective of the present study was to reveal the potential of S. androgynus strip to be used as a medicinal drug. Materials and methods. The antimicrobial properties of S. androgynus stem extract against Aeromonas hydrophila, Escherichia coli, Edwardsiella tarda, Flavobacterium sp., Klebsiella sp., Salmonella sp., Vibrio alginolyticus, V. parahaemolyticus, V. cholerae and Pseudomonas aeruginosa were revealed by using the broth micro-dilution method, whereas the anticancer effects of the extract was determined with a colorimetric MTT (tetrazolium) assay against human breast adenocarcinoma (MCF-7). The antioxidant activity of the plant extract was characterized also by using a α, α-diphenyl-β-picrylhydrazyl (DPPH) radical scavenging method. Finally, chemical compounds of the plant extract were screened and identified by using gas chromatography – mass spectrometry (GC–MS). Results. The minimum inhibitory concentration (MIC) values ranged from 7.81 to 62.5 mg/l in which the plant extract was found to inhibit the growth of Edwardsiella tarda, Escherichia coli, Flavobacterium sp., Pseudomonas aeruginosa and Vibrio cholerae at 7.81 mg/l, Klebsiella sp., Aeromonas hydrophila and Vibrio alginolyticus at 15.6 mg/l, and it was able to control the growth of Salmonella sp. and Vibrio parahaemolyticus at 62.5 mg/l. The results of the present study has shown S. androgynus stem extract to possess a high antimicrobial activity and a moderate antioxidant activity (inhibition concentration 50%; IC50 of DPPH is 8 ppt), but no anticancer activity. A total of 34 compounds were identified in the plant extract in which the major compounds were 9, 12, 15-octadecatrienoic acid, methyl ester, (Z, Z, Z)- (14.48%) and phytol (13.08%). Conclusions. S. androgynus stem extract can be used as an antimicrobial and antioxidant agents. Keywords: antimicrobial, antioxidant, anticancer, chemical compound, Sauropus androgynus stem

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Development of a Multiplex-PCR tool for the simultaneous detection of Vibrio alginolyticus, Vibrio anguillarum, Vibrio harveyi and Edwardsiella tarda

Frontiers in Marine Science ◽

10.3389/conf.fmars.2016.04.00028 ◽

2016 ◽

Vol 3 ◽

Author(s):

Pinto Micaela ◽

Baptista Teresa ◽

Afonso Clélia

Keyword(s):

Multiplex Pcr ◽

Vibrio Harveyi ◽

Simultaneous Detection ◽

Vibrio Anguillarum ◽

Vibrio Alginolyticus ◽

Edwardsiella Tarda

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Exemplar Abstract for Edwardsiella tarda Ewing et al. 1965 (Approved Lists 1980) pro synon. Edwardsiella anguillimortifera (Hoshina 1962) Sakazaki and Tamura 1975 (Approved Lists 1980).

The NamesforLife Abstracts ◽

10.1601/ex.15559 ◽

2003 ◽

Author(s):

Charles Thomas Parker ◽

Dorothea Taylor ◽

George M Garrity

Keyword(s):

Edwardsiella Tarda

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Viability of Edwardsiella tarda and Esherichia coli preserved with glycerol-tryptone soy broth (TSB) kept at freezing temperature

AQUATIC SCIENCE & MANAGEMENT ◽

10.35800/jasm.1.2.2013.7278 ◽

2013 ◽

Vol 1 (2) ◽

pp. 154

Author(s):

Abdur Rohman ◽

Frans Ijong ◽

I K Suwetja

Keyword(s):

Research And Development ◽

Germ Plasm ◽

Freezing Temperature ◽

Experimental Methods ◽

Edwardsiella Tarda ◽

Diagnostic Tools ◽

Glycerol Concentration ◽

E Coli ◽

Esherichia Coli ◽

Escherchia Coli

Preservation of bacteria carried out in relation to the collection and preservation of germ plasm microbe is useful for research and development or for the establishment of diagnostic tools. Glycerol is a good preservation media but it is not known what doses should be used for effective preservation. This research used two experimental methods consisting of 2 factors and 3 treatments. This study aimed to find the best glycerol concentration that can be used to preserve Edwarsiella tarda and Escherchia coli in the -20ºC environment, to understand the viability of bacteria after being preserved and to describe the characteristics of the preserved bacteria. Treatments applied were 10%, 15% and 20% glycerol in TSB. Viability of the bacteria was analyzed after 7, 14, 28, 35, and 42 days of preservation. Results showed that E.coli bacteria preserved in 15% glycerol had the highest viability, i.e. 84% and preserved in 10% glycerol had the lowest viability, i.e. 80%. But for E. tarda bacteria preserved in 10% glycerol had the highest viability, i.e. 1.83% and preseved in 15% glycerol had the lowest viability, i.e. 0,55%. Preservasi bakteri dilakukan dalam kaitannya dengan koleksi dan konservasi plasma nutfah mikroba yang berguna untuk penelitian dan pengembangan atau untuk pembentukan alat diagnosa. Gliserol merupakan bahan preservasi yang baik, tetapi belum diketahui dosis yang baik dan efektif untuk preservasi bakteri Edwarsiella tarda dan Escherchia coli pada suhu -20ºC. Penelitian ini dilakukan dengan metode eksperimen yang terdiri dari 2 faktor dan 3 taraf perlakuan, masing-masing perlakuan dengan 3 kali ulangan, media preservasi yang digunakan adalah TSB dan gliserol dengan konsentrasi 10%, 15% dan 20%. Parameter yang diukur adalah viabilitas dan kecocokan/penyimpangan karakteristik biokimia. Penelitian ini dilaksanakan di Laboratorium Balai Karantina Ikan Pengendalian Mutu dan Keamanan Hasil Perikanan Manado, dari bulan September sampai dengan November 2013. Tujuan Penelitian ini adalah untuk menentukan konsentrasi gliserol dalam TSB sebagai media preservasi yang efektif dan efisien pada bakteri Edwarsiella tarda dan Escherchia coli yang dipreservasi dengan suhu -20ºC dan disimpan selama 42 hari. Hasil penelitian menunjukkan adanya penurunan laju pertumbuhan bakteri selama preservasi. Persentase viabilitas bakteri E. coli yang tertinggi selama preservasi diperoleh dengan penggunaan gliserol konsentrasi 15% dengan jumlah 84% dan yang terendah adalah dengan penggunaan konsentrasi 10% yakni sebesar 80%, sedangkan untuk E. tarda persentase viabilitas bakteri yang tertinggi selama preservasi diperoleh dengan penggunaan gliserol konsentrasi 10% dengan jumlah 1,83% dan yang terendah adalah dengan penggunaan konsentrasi 15% yakni sebesar 0,55%. Berdasarkan uji statistik analisis variasi (ANAVA) didapat hasil F hitung E. tarda dan E. coli yang lebih besar dari FTabel dengan tingkat kepercayaan 95 %.

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