Potential role of rainbow trout red blood cells as mediators in the immune response induced by DNA vaccines

2019 ◽  
Vol 91 ◽  
pp. 425
Author(s):  
S. Puente-Marin ◽  
I. Nombela ◽  
V. Chico ◽  
S. Ciordia ◽  
M.C. Mena ◽  
...  
Keyword(s):  
Immune Response ◽  
Rainbow Trout ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Potential Role ◽  
Dna Vaccines

scholarly journals Integrated Transcriptomic and Proteomic Analysis of Red Blood Cells from Rainbow Trout Challenged with VHSV Point Towards Novel Immunomodulant Targets

Vaccines ◽  
2019 ◽  
Vol 7 (3) ◽  
pp. 63 ◽  
Author(s):  
Nombela ◽  
Lopez-Lorigados ◽  
Salvador-Mira ◽  
Puente-Marin ◽  
Chico ◽  
...  
Keyword(s):  
Immune Response ◽  
Rainbow Trout ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Antigen Processing ◽  
Head Kidney ◽  
Interferon Response ◽  
Type I ◽  
Fish Viruses

Teleost red blood cells (RBCs) are nucleated and therefore can propagate cellular responses to exogenous stimuli. RBCs can mount an immune response against a variety of fish viruses, including the viral septicemia hemorrhagic virus (VHSV), which is one of the most prevalent fish viruses resulting in aquaculture losses. In this work, RBCs from blood and head kidney samples of rainbow trout challenged with VHSV were analyzed via transcriptomic and proteomic analyses. We detected an overrepresentation of differentially expressed genes (DEGs) related to the type I interferon response and signaling in RBCs from the head kidney and related to complement activation in RBCs from blood. Antigen processing and presentation of peptide antigen was overrepresented in RBCs from both tissues. DEGs shared by both tissues showed an opposite expression profile. In summary, this work has demonstrated that teleost RBCs can modulate the immune response during an in vivo viral infection, thus implicating RBCs as cell targets for the development of novel immunomodulants.

NKEF, an antiviral protein involved in the immune response of rainbow trout red blood cells

2019 ◽  
Vol 91 ◽  
pp. 409
Author(s):  
M. Salvador-Mira ◽  
V. Chico ◽  
I. Nombela ◽  
S. Puente-Marin ◽  
S. Ciordia ◽  
...  
Keyword(s):  
Immune Response ◽  
Rainbow Trout ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Antiviral Protein

scholarly journals Infectious pancreatic necrosis virus triggers antiviral immune response in rainbow trout red blood cells, despite not being infective

F1000Research ◽  
2017 ◽  
Vol 6 ◽  
pp. 1968 ◽  
Author(s):  
Ivan Nombela ◽  
Aurora Carrion ◽  
Sara Puente-Marin ◽  
Veronica Chico ◽  
Luis Mercado ◽  
...  
Keyword(s):  
Immune Response ◽  
Rainbow Trout ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Pancreatic Necrosis ◽  
Antiviral Response ◽  
Infectious Pancreatic Necrosis Virus ◽  
Necrosis Virus ◽  
Infectious Pancreatic Necrosis ◽  
Pancreatic Necrosis Virus

Background: Some fish viruses, such as piscine orthoreovirus and infectious salmon anemia virus, target red blood cells (RBCs), replicate inside them and induce an immune response. However, the roles of RBCs in the context of infectious pancreatic necrosis virus (IPNV) infection  have not been studied yet.Methods: Ex vivo rainbow trout RBCs were obtained from peripheral blood, Ficoll purified and exposed to IPNV in order to analyze infectivity and immune response using RT-qPCR, immune fluorescence imaging, flow cytometry and western-blotting techniques.Results: IPNV could not infect RBCs; however, IPNV increased the expression of the INF1-related genesifn-1,pkrandmxgenes. Moreover, conditioned media from IPNV-exposed RBCs conferred protection against IPNV infection in CHSE-214 fish cell line.Conclusions: Despite not being infected, rainbow trout RBCs could respond to IPNV with increased expression of antiviral genes. Fish RBCs could be considered as mediators of the antiviral response and therefore targets of new strategies against fish viral infections. Further research is ongoing to completely understand the molecular mechanism that triggers this antiviral response in rainbow trout RBCs.

scholarly journals Lenalidomide – A Brief Review of its Use in Myelodysplastic Syndromes

2015 ◽  
Vol 11 (2) ◽  
pp. 141
Author(s):  
Jaykumar J Sejpal ◽  
Yogesh R Belagali ◽  
Hanmant V Barkate ◽  
◽  
◽  
...  
Keyword(s):  
Older People ◽  
Red Blood Cells ◽  
Myelodysplastic Syndromes ◽  
Blood Cells ◽  
Lower Risk ◽  
Potential Role ◽  
Chromosome 5 ◽  
Blood Diseases ◽  
Transfusion Independence

Myelodysplastic syndromes (MDS) are a heterogeneous group of blood diseases mainly affecting older people. Deletion of the long arm of chromosome 5 (del[5q]) is reported in approximately 10–15 % of all MDS cases. Lenalidomide is an immunomodulatory, anti-cytokine and anti-angiogenic agent, which leads to red blood cells transfusion independence in patients of lower risk MDS with del(5q). This review briefly describes role of lenalidomide in treatment of lower-risk MDS with del(5q) as well as non-del-5q MDS. Recent evidence also suggest a potential role of lenalidomide in combination with other agents for treatment of higher-risk MDS

scholarly journals Identification of diverse defense mechanisms in rainbow trout red blood cells in response to halted replication of VHS virus

F1000Research ◽  
2018 ◽  
Vol 6 ◽  
pp. 1958 ◽  
Author(s):  
Ivan Nombela ◽  
Sara Puente-Marin ◽  
Veronica Chico ◽  
Alberto J. Villena ◽  
Begoña Carracedo ◽  
...  
Keyword(s):  
Immune Response ◽  
Rainbow Trout ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Antiviral Response ◽  
Protein Profiling ◽  
N Gene ◽  
Type I ◽  
Ongoing Research ◽  
Gene Transcripts

Background:It has been described that fish nucleated red blood cells (RBCs) generate a wide variety of immune-related gene transcripts when viruses highly replicate inside them and are their main target cell. The immune response and mechanisms of fish RBCs against viruses targeting other cells or tissues has not yet been explored and is the objective of our study.Methods:Rainbow trout RBCs were obtained from peripheral blood, ficoll purified and exposed toViral Haemorrhagic Septicaemia virus(VHSV). Immune response was evaluated by means of RT-qPCR, flow cytometry, immunofluorescence and isobaric tag for relative and absolute quantification (iTRAQ) protein profiling.Results:VHSV N gene transcripts incremented early postexposure and were drastically decreased after 6 hours postexposure (hpe). The expression of type I interferon (ifn1) gene was significantly downregulated at early postexposure (3 hpe), together with a gradual downregulation of interferon-induciblemxandpkrgenes until 72 hpe. Type I IFN protein was downregulated and interferon-inducible Mx protein was maintained at basal levels. Co-culture assays of RBCs, previously exposed to UV-inactivated VHSV, and TSS (stromal cell line from spleen) revealed IFN crosstalk between both cell types. On the other hand, anti-microbial peptide β-defensin 1 and neutrophil chemotactic factor interleukin 8 were slightly upregulated in VHSV-exposed RBCs. iTRAQ profiling revealed that VHSV exposure can induce a global protein downregulation in rainbow trout RBCs, mainly related to RNA stability and proteasome pathways. Antioxidant/antiviral response is also suggested to be involved in the response of rainbow trout RBCs to VHSV.Conclusions:A variety of mechanisms are proposed to be implicated in the antiviral response of rainbow trout RBCs against VHSV halted infection. Ongoing research is focused on understanding the mechanisms in detail.

scholarly journals Short Circuiting of the Ocular Oxygen Concentrating Mechanism in the Teleost Salmo gairdneri Using Carbonic Anhydrase Inhibitors

1974 ◽  
Vol 64 (3) ◽  
pp. 263-273 ◽  
Author(s):  
Michael B. Fairbanks ◽  
J. Russell Hoffert ◽  
Paul O. Fromm
Keyword(s):  
Rainbow Trout ◽  
Carbonic Anhydrase ◽  
Red Blood Cells ◽  
Oxygen Concentration ◽  
Blood Cells ◽  
Salmo Gairdneri ◽  
Retinal Tissue ◽  
Rete Mirabile ◽  
Counter Current

Ocular oxygen concentration by the process of counter current multiplication in rainbow trout (Salmo gairdneri) was rapidly suppressed after intraperitoneal injections of the carbonic anhydrase inhibitor CL-11,366. The rapidity with which this drug acted suggested a short circuiting of the choroidal rete mirabile. A comparison was made between the time after injection of inhibitor at which oxygen concentrating ability was lost to the time after injection of inhibitor at which its presence in red blood cells, choroidal rete, pseudobranch, and retinal tissue was first noted. A scheme for the possible role of carbonic anhydrase from each of these tissues in the process of ocular oxygen concentration is given.

Sign in / Sign up

Export Citation Format

Share Document