Effect of seasonal variance on intestinal epithelial barriers and the associated innate immune response of the small intestine of the Chinese soft-shelled turtles

Lactobacillus delbrueckii subsp. lactis CRL 581 beneficially modulates the intestinal antiviral innate immune response triggered by the Toll-like receptor 3 (TLR3) agonist poly(I:C) in vivo. This study aimed to characterize further the immunomodulatory properties of the technologically relevant starter culture L. delbrueckii subsp. lactis CRL 581 by evaluating its interaction with intestinal epithelial cells and macrophages in the context of innate immune responses triggered by TLR3. Our results showed that the CRL 581 strain was able to adhere to porcine intestinal epithelial (PIE) cells and mucins. The CRL 581 strain also augmented the expression of antiviral factors (IFN-α, IFN-β, Mx1, OAS1, and OAS2) and reduced inflammatory cytokines in PIE cells triggered by TLR3 stimulation. In addition, the influence of L. delbrueckii subsp. lactis CRL 581 on the response of murine RAW macrophages to the activation of TLR3 was evaluated. The CRL 581 strain was capable of enhancing the expression of IFN-α, IFN-β, IFN-γ, Mx1, OAS1, TNF-α, and IL-1β. Of note, the CRL 581 strain also augmented the expression of IL-10 in macrophages. The results of this study show that the high proteolytic strain L. delbrueckii spp. lactis CRL 581 was able to beneficially modulate the intestinal innate antiviral immune response by regulating the response of both epithelial cells and macrophages relative to TLR3 activation.

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O-017 HIF-mediated Autophagy Contributes to Intestinal Epithelial Cell-intrinsic Innate Immune Response Against Invasive Bacteria

Inflammatory Bowel Diseases ◽

10.1097/01.mib.0000438568.18012.49 ◽

2013 ◽

Vol 19 ◽

pp. S12 ◽

Cited By ~ 1

Author(s):

Louise Glover ◽

Bejan Saeedi ◽

Caleb Kelly ◽

Eric Campbell ◽

Amanda Bayless ◽

...

Keyword(s):

Immune Response ◽

Epithelial Cell ◽

Innate Immune Response ◽

Intestinal Epithelial Cell ◽

Innate Immune ◽

Intestinal Epithelial

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T1244 Gap Junction Intercellular Communication Plays a Central Role in the Innate Immune Response of the Intestinal Epithelial Barrier

Gastroenterology ◽

10.1016/s0016-5085(08)62401-4 ◽

2008 ◽

Vol 134 (4) ◽

pp. A-514-A-515

Author(s):

Anna Nierobis ◽

Daniel K. Podolsky ◽

Elke Cario

Keyword(s):

Immune Response ◽

Gap Junction ◽

Innate Immune Response ◽

Intercellular Communication ◽

Innate Immune ◽

Epithelial Barrier ◽

Intestinal Epithelial ◽

Intestinal Epithelial Barrier ◽

Gap Junction Intercellular Communication

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Lactobacilli stimulate the innate immune response and modulate the TLR expression of HT29 intestinal epithelial cells in vitro

International Journal of Food Microbiology ◽

10.1016/j.ijfoodmicro.2009.05.013 ◽

2009 ◽

Vol 133 (1-2) ◽

pp. 86-93 ◽

Cited By ~ 77

Author(s):

María G. Vizoso Pinto ◽

Manuel Rodriguez Gómez ◽

Stephanie Seifert ◽

Bernhard Watzl ◽

Wilhelm H. Holzapfel ◽

...

Keyword(s):

Immune Response ◽

Epithelial Cells ◽

Innate Immune Response ◽

Intestinal Epithelial Cells ◽

Innate Immune ◽

Intestinal Epithelial

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Colostrum and bioactive, colostral peptides differentially modulate the innate immune response of intestinal epithelial cells

Journal of Peptide Science ◽

10.1002/psc.1190 ◽

2009 ◽

Vol 16 (1) ◽

pp. 21-30 ◽

Cited By ~ 23

Author(s):

Ann Louise Worsøe Jørgensen ◽

Helle Risdahl Juul-Madsen ◽

Jan Stagsted

Keyword(s):

Immune Response ◽

Epithelial Cells ◽

Innate Immune Response ◽

Intestinal Epithelial Cells ◽

Innate Immune ◽

Intestinal Epithelial

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Development of an in vitro immunobiotic evaluation system against rotavirus infection in bovine intestinal epitheliocytes

Beneficial Microbes ◽

10.3920/bm2016.0155 ◽

2017 ◽

Vol 8 (2) ◽

pp. 309-321 ◽

Cited By ~ 9

Author(s):

H. Kobayashi ◽

P. Kanmani ◽

T. Ishizuka ◽

A. Miyazaki ◽

J. Soma ◽

...

Keyword(s):

Immune Response ◽

Innate Immune Response ◽

Evaluation System ◽

Innate Immune ◽

Poly I:C ◽

Epithelial Cell Line ◽

Intestinal Epithelial ◽

Antiviral Immune Response ◽

Comprehensive Information

The bovine intestinal epithelial cell line (BIE cells) expresses the Toll-like receptor (TLR)3 and is able to mount an antiviral immune response after the stimulation with poly(I:C). In the present study, we aimed to further characterise the antiviral defence mechanisms in BIE cells by evaluating the innate immune response triggered by rotavirus (RV) infection. In addition, we attempted to determine whether immunobiotic bifidobacteria are able to confer protection of BIE cells against RV infection by beneficially modulating the antiviral immune response. RV OSU (porcine) and UK (bovine) effectively infected BIE cells, while a significant lower capacity to infect BIE cells was observed for human (Wa) and murine (EW) RV. We observed that viral infection in BIE cells triggered TLR3/RIG-I-mediated immune responses with activation of IRF3 and TRAF3, induction of interferon beta (IFN-β) and up-regulation of inflammatory cytokines. Our results also demonstrated that preventive treatments with Bifidobacterium infantis MCC12 or Bifidobacterium breve MCC1274 significantly reduced RV titres in infected BIE cells and differentially modulated the innate immune response. Of note, both strains significantly improved the production of the antiviral factor IFN-β in RV-infected BIE cells. In conclusion, this work provides comprehensive information on the antiviral immune response of BIE cells against RV, that can be further studied for the development of strategies aimed to improve antiviral defences in bovine intestinal epithelial cells. Our results also demonstrate that BIE cells could be used as a newly immunobiotic evaluation system against RV infection for application in the bovine host.

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Modulation of Toll-like receptor-mediated innate immunity in bovine intestinal epithelial cells by lactic acid bacteria isolated from feedlot cattle

Beneficial Microbes ◽

10.3920/bm2019.0189 ◽

2020 ◽

Vol 11 (3) ◽

pp. 269-282 ◽

Cited By ~ 1

Author(s):

F. Mansilla ◽

M. Takagi ◽

V. Garcia-Castillo ◽

H. Aso ◽

M.E. Nader-Macias ◽

...

Keyword(s):

Immune Response ◽

Innate Immune Response ◽

Feedlot Cattle ◽

Innate Immune ◽

Enterotoxigenic Escherichia Coli ◽

Type I ◽

Intestinal Epithelial ◽

Negative Regulators ◽

Heat Stable ◽

Mitogen Activated Protein

The ability of lactobacilli isolated from feedlot cattle environment to differentially modulate the innate immune response triggered by Toll-like receptors (TLRs) activation in bovine intestinal epithelial (BIE) cells was evaluated. BIE cells were stimulated with Lactobacillus mucosae CRL2069, Lactobacillus acidophilus CRL2074, Lactobacillus fermentum CRL2085 or Lactobacillus rhamnosus CRL2084 and challenged with heat-stable pathogen associated molecular patterns (PAMPs) from enterotoxigenic Escherichia coli (ETEC) to induce the activation of TLR4 or with polyinosinic:polycytidylic acid (poly(I:C)) to activate TLR3. Type I interferons, cytokines, chemokines and negative regulators of TLR signalling were studied by RT-PCR. L. mucosae CRL2069 significantly reduced the expression of interleukin (IL)-8 and monocyte chemoattractant protein (MCP)-1 in BIE cells in the context of TLR3 activation. L. mucosae CRL2069 also reduced the expression of tumour necrosis factor-α, IL-β, MCP-1, and IL-8 in heat-stable ETEC PAMPs-challenged BIE cells. In addition, reduced expressions of IL-6, MCP-1, and IL-8 were found in BIE cells stimulated with L. rhamnosus CRL2084, although its effect was significantly lower than that observed for the CRL2069 strain. The reduced levels of pro-inflammatory factors in BIE cells induced by the CRL2069 and CRL2085 strains was related to their ability of increasing the expression of TLR negative regulators. L. mucosae CRL2069 significantly improved the expression of A20-binding inhibitor of NFκ-B activation 3 (ABIN-3), interleukin-1 receptor-associated kinase M (IRAK-M) and mitogen-activated protein kinase 1 (MKP-1) while L. rhamnosus CRL2084 augmented ABIN-3 expression in BIE cells. The results of this work suggest that among the studied strains, L. mucosae CRL2069 was able to regulate TLR3-mediated innate immune response and showed a remarkable capacity to modulate TLR4-mediated inflammation in BIE cells. The CRL2069 strain induce the up-regulation of three TLR negative regulators that would influence nuclear factor kB and mitogen-activated protein kinases signalling pathways while reducing the expression of pro-inflammatory cytokines and chemokines. Therefore, L. mucosae CRL2069 is an interesting immunobiotic candidate for the protection of the bovine host against TLR-mediated intestinal inflammatory damage.

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