Variation in virulence of Beauveria bassiana and B. pseudobassiana to the pine weevil Pissodes nemorensis in relation to mycelium characteristics and virulence genes

2017 ◽  
Vol 121 (2) ◽  
pp. 189-197 ◽  
Author(s):  
Pedro Romón ◽  
Hardus Hatting ◽  
Arturo Goldarazena ◽  
Juan Carlos Iturrondobeitia
Keyword(s):  
Beauveria Bassiana ◽  
Virulence Genes ◽  
Pine Weevil ◽  
Pissodes Nemorensis

Potential of Beauveria bassiana application via a carrier to control the large pine weevil

2021 ◽  
Vol 143 ◽  
pp. 105563
Author(s):  
Michal Lalík ◽  
Juraj Galko ◽  
Christo Nikolov ◽  
Slavomír Rell ◽  
Andrej Kunca ◽  
...  
Keyword(s):  
Beauveria Bassiana ◽  
Pine Weevil

Pine Weevil (Coleoptera: Curculionidae) Population Monitoring in Christmas Trees Using Volatile Host Compounds

2000 ◽  
Vol 35 (2) ◽  
pp. 167-175 ◽  
Author(s):  
L. K. Rieske
Keyword(s):  
Population Monitoring ◽  
Ground Level ◽  
Pitfall Traps ◽  
Host Plant Volatiles ◽  
Trap Catch ◽  
North Central ◽  
Pine Weevil ◽  
Christmas Trees ◽  
Pissodes Nemorensis ◽  
Hylobius Pales

The eastern pine weevil, Pissodes nemorensis Germar, and the pales weevil, Hylobius pales (Herbst), are major pests of pine production in eastern North America. Ethanol- and turpentine-baited pitfall traps and flight traps, and pit traps baited with fresh pine billets, were used to characterize weevil species composition in north-central Kentucky (USA) and assess seasonal activity by exploiting the weevils' attraction to host plant volatiles. During the 1998 growing season, P. nemorensis was the predominant species, comprising over 95% of the total trap catch for the season. Weevils were most responsive to fresh pine billets in pit traps, followed by ethanol- and turpentine-baited flight traps, and ethanol- and turpentine-baited pitfall traps. The sex ratio of P. nemorensis captured in pit traps was male biased, and in pitfall traps it was weakly female biased. Flight trap catch in traps placed 0.8 m above ground level was female biased. More weevils were captured in flight traps at 0.8 m than in flight traps at 1.6 m. Catches were greatest in traps placed perpendicular to the slope. Although H. pales was present at the site, numbers captured were too low to statistically assess trap efficacy.

scholarly journals The role of fungus Beauveria bassiana in reducing the number of Pissodes castaneus (Col., Curculionidae) in young forests

2016 ◽  
Vol 58 (4) ◽  
pp. 214-219 ◽  
Author(s):  
Iwona Skrzecz ◽  
Elżbieta Popowska-Nowak ◽  
Robert Wolski ◽  
Alicja Sowińska ◽  
Tomasz Jabłoński ◽  
...  
Keyword(s):  
Biological Activity ◽  
Beauveria Bassiana ◽  
Field Evaluation ◽  
Median Lethal Concentration ◽  
Pine Weevil ◽  
Insect Mortality ◽  
Environmentally Safe ◽  
Young Forests ◽  
Fungus Beauveria Bassiana

Abstract Small banded pine weevil Pissodes castaneus is one of the most dangerous pests of Pinus sylvestris plantations and thickets. The lack of effective and environmentally safe methods of limiting the number of the pest justified to undertake the studies aimed at the laboratory and field evaluation of biological activity of entomopathogenic fungus Beauveria bassiana used to reduce the numbers of small banded pine weevil. In laboratory, the beetles were reared on the sections of fresh Scots pine twigs that were treated with five suspensions containing from 1 × 104 to 1 × 108 conidia of B. bassiana in 1 ml. During the 3-week rearing, insect mortality was determined and median lethal concentration LC50 was calculated. The field treatments consisted of spraying 4-year-old P. sylvestris trees with two formulations of fungus containing 1 × 108 conidia ml−1 of suspension. Treatments consisted of spraying 4-year-old P. sylvestris trees with two fungus formulations containing 1 × 108 conidia ml−1. High insecticidal activity of B. bassiana was found because the pathogen caused the death of 14-94% of P. castaneus beetles, LC50 = 6.51 × 105 conidia ml−1. Field treatments did not result in the reduction of plant damage caused by small banded pine weevil; therefore, the spraying of trees with B. bassiana cannot be recommended to protect the young stands of P. sylvestris against pest.

Potential of Beauveria bassiana (Hyphomycetes: Moniliales) for controlling the white pine weevil, Pissodes strobi (Col., Curculionidae)

2007 ◽  
Vol 131 (2) ◽  
pp. 90-97 ◽  
Author(s):  
R. Trudel ◽  
R. Lavallée ◽  
C. Guertin ◽  
C. Côté ◽  
S. I. Todorova ◽  
...  
Keyword(s):  
Beauveria Bassiana ◽  
White Pine ◽  
Pissodes Strobi ◽  
Pine Weevil ◽  
White Pine Weevil

Vibrio harveyi virulence gene expression in vitro and in vivo during infection in black tiger shrimp Penaeus monodon

2020 ◽  
Vol 139 ◽  
pp. 153-160
Author(s):  
S Peeralil ◽  
TC Joseph ◽  
V Murugadas ◽  
PG Akhilnath ◽  
VN Sreejith ◽  
...  
Keyword(s):  
Gene Expression ◽  
Virulence Genes ◽  
Vibrio Harveyi ◽  
Penaeus Monodon ◽  
Challenge Test ◽  
Virulence Gene ◽  
Economic Losses ◽  
Virulence Gene Expression

Luminescent Vibrio harveyi is common in sea and estuarine waters. It produces several virulence factors and negatively affects larval penaeid shrimp in hatcheries, resulting in severe economic losses to shrimp aquaculture. Although V. harveyi is an important pathogen of shrimp, its pathogenicity mechanisms have yet to be completely elucidated. In the present study, isolates of V. harveyi were isolated and characterized from diseased Penaeus monodon postlarvae from hatcheries in Kerala, India, from September to December 2016. All 23 tested isolates were positive for lipase, phospholipase, caseinase, gelatinase and chitinase activity, and 3 of the isolates (MFB32, MFB71 and MFB68) showed potential for significant biofilm formation. Based on the presence of virulence genes, the isolates of V. harveyi were grouped into 6 genotypes, predominated by vhpA+ flaB+ ser+ vhh1- luxR+ vopD- vcrD+ vscN-. One isolate from each genotype was randomly selected for in vivo virulence experiments, and the LD50 ranged from 1.7 ± 0.5 × 103 to 4.1 ± 0.1 × 105 CFU ml-1. The expression of genes during the infection in postlarvae was high in 2 of the isolates (MFB12 and MFB32), consistent with the result of the challenge test. However, in MFB19, even though all genes tested were present, their expression level was very low and likely contributed to its lack of virulence. Because of the significant variation in gene expression, the presence of virulence genes alone cannot be used as a marker for pathogenicity of V. harveyi.

Actividad de dos hongos entomopatógenos, identificados molecularmente, sobre Bactericera cockerelli

2017 ◽  
Vol 43 (1) ◽  
pp. 27 ◽  
Author(s):  
Fabiola Villegas-Rodríguez ◽  
Ovidio Díaz-Gómez ◽  
J. Sergio Casas-Flores ◽  
Clara Teresa Monreal-Vargas ◽  
Fernando Tamayo-Mejía ◽  
...  
Keyword(s):  
Beauveria Bassiana ◽  
Bactericera Cockerelli ◽  
Candidatus Liberibacter

El psílido de la papa Bactericera cockerelli afecta a las solanáceas en México, por el daño directo que causa, además, de que es transmisor de patógenos procariotes como Candidatus Liberibacter solanacerum (psyllaurous). Este insecto es combatido principalmente con productos químicos. Sin embargo, en la actualidad, se buscan nuevas estrategias para su manejo como el uso de hongos entomopatógenos. El objetivo de este trabajo fue evaluar la susceptibilidad del psílido de la papa a dos cepas comerciales y dos nativas de Metarizhium anisopliae y Beauveria bassiana, así como confirmar la identificación de las mismas mediante técnicas moleculares. Los bioensayos se realizaron con la cepa nativa MA25 de M. anisopliae y las comerciales Metabich® (MA28) y Bassianil® (BB09) y la nativa BB42 de Be. bassiana. Se probaron cinco concentraciones sobre ninfas del tercer estadio de B. cockerelli y se obtuvieron sus CL50 y CL95. Todas las cepas resultaron patógenas para el psílido de la papa, las más tóxicas fueron BB09, BB42 y MA28. La mortalidad fluctuó entre 90 a 100% con las concentraciones más altas de cada cepa: la más virulenta fue BB09, con una CL50 de 2,99 x 104 conidias mL-1 y MA25 la menos virulenta tuvo una CL50 de 6,34 x 105 conidias ml-1. Mediante la clonación y la secuenciación de la región ITS de los rADN 18S, se corroboró que las cepas nativas identificadas por morfología microscópica y claves taxonómicas corresponden a Be. bassiana y M. anisopliae.

Beauveria bassiana xylanase: a biomass degrading enzyme from an entomopathogen

2020 ◽  
Author(s):  
Santhosh Pillai ◽  
AYODEJI EMMANUEL AMOBONYE ◽  
Prashant Bhagwat
Keyword(s):  
Beauveria Bassiana ◽  
Degrading Enzyme

Evaluación de hongos entomopatógenos para el control biológico del mosquito Culex quinquefasciatus

2009 ◽  
Vol 5 (9) ◽  
pp. 25-38
Author(s):  
Martha Giselle Rivera Pineda ◽  
Sandra Patricia Garzón Lozano ◽  
Luz Inés Villarreal Salazar
Keyword(s):  
Beauveria Bassiana ◽  
Culex Quinquefasciatus ◽  
Metarhizium Anisopliae

En busca de una alternativa de control del mosquito Culex quinquefasciatus en áreas urbanas se desarrolló el proyecto: “Evaluación de microorganismos biocontroladores sobre el mosquito Culex quinquefasciatus” cuyo objetivo fue evaluar la capacidad biocontroladora de diferentes hongos entomopatógenos sobre larvas del mosquito a nivel de laboratorio. Se evaluaron 16 aislamientos de hongos en diferentes concentraciones. Se constataron diferencias en el porcentaje de mortalidad producido en larvas de Cx. quinquefasciatus dependientes del tipo de aislamiento, de la concentración utilizada y del tiempo de exposición. Los aislamientos que presentaron el nivel de mortalidad más alto pertenecen a la especie Metarhizium anisopliae, seguidos por aislamientos de la especie Beauveria bassiana. El aislamiento que presentó el mayor nivel de mortalidad (100%) en menor tiempo de exposición (24 horas) fue Metarhizium anisopliae 99068 en una concentración de 1x106 e/ml. Por lo tanto este aislamiento es recomendado para su evaluación en campo y desarrollo de un producto comercial.

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