Comparing Exogenous Methods to Induce Plant-Resistance Against a Bark-Feeding Insect

Exogenous application of the plant hormone methyl jasmonate (MeJA) can trigger induced plant defenses against herbivores, and has been shown to provide protection against insect herbivory in conifer seedlings. Other methods, such as mechanical damage to seedlings, can also induce plant defenses, yet few have been compared to MeJA and most studies lack subsequent herbivory feeding tests. We conducted two lab experiments to: (1) compare the efficacy of MeJA to mechanical damage treatments that could also induce seedling resistance, (2) examine if subsequent insect damage differs depending on the time since induction treatments occurred, and (3) assess if these induction methods affect plant growth. We compared Scots pine (Pinus sylvestris) seedlings sprayed with MeJA (10 or 15 mM) to seedlings subjected to four different mechanical bark damage treatments (two different bark wound sizes, needle-piercing damage, root damage) and previous pine weevil (Hylobius abietis) damage as a reference treatment. The seedlings were exposed to pine weevils 12 or 32 days after treatments (early and late exposure, hereafter), and resistance was measured as the amount of damage received by plants. At early exposure, seedlings treated with needle-piercing damage received significantly more subsequent pine weevil feeding damage than those treated with MeJA. Seedlings treated with MeJA and needle-piercing damage received 84% less and 250% more pine weevil feeding, respectively, relative to control seedlings. The other treatments did not differ statistically from control or MeJA in terms of subsequent pine weevil damage. For the late exposure group, plants in all induction treatments tended to receive less pine weevil feeding (yet this was not statistically significant) compared to control seedlings. On the other hand, MeJA significantly slowed down seedling growth relative to control and all other induction treatments. Overall, the mechanical damage treatments appeared to have no or variable effects on seedling resistance. One of the treatments, needle-piercing damage, actually increased pine weevil feeding at early exposure. These results therefore suggest that mechanical damage shows little potential as a plant protection measure to reduce feeding by a bark-chewing insect.

Variation in Methyl Jasmonate-Induced Defense Among Norway Spruce Clones and Trade-Offs in Resistance Against a Fungal and an Insect Pest

An essential component of plant defense is the change that occurs from a constitutive to an induced state following damage or infection. Exogenous application of the plant hormone methyl jasmonate (MeJA) has shown great potential to be used as a defense inducer prior to pest exposure, and could be used as a plant protection measure. Here, we examined (1) the importance of MeJA-mediated induction for Norway spruce (Picea abies) resistance against damage by the pine weevil Hylobius abietis, which poses a threat to seedling survival, and infection by the spruce bark beetle-associated blue-stain fungus Endoconidiophora polonica, (2) genotypic variation in MeJA-induced defense (terpene chemistry), and (3) correlations among resistance to each pest. In a semi-field experiment, we exposed rooted-cuttings from nine different Norway spruce clones to insect damage and fungal infection separately. Plants were treated with 0, 25, or 50 mM MeJA, and planted in blocks where only pine weevils were released, or in a separate block in which plants were fungus-inoculated or not (control group). As measures of resistance, stem area debarked and fungal lesion lengths were assessed, and as a measure of defensive capacity, terpene chemistry was examined. We found that MeJA treatment increased resistance to H. abietis and E. polonica, but effects varied with clone. Norway spruce clones that exhibited high constitutive resistance did not show large changes in area debarked or lesion length when MeJA-treated, and vice versa. Moreover, insect damage negatively correlated with fungal infection. Clones receiving little pine weevil damage experienced larger lesion lengths, and vice versa, both in the constitutive and induced states. Changes in absolute terpene concentrations occurred with MeJA treatment (but not on proportional terpene concentrations), however, variation in chemistry was mostly explained by differences between clones. We conclude that MeJA can enhance protection against H. abietis and E. polonica, but the extent of protection will depend on the importance of constitutive and induced resistance for the Norway spruce clone in question. Trade-offs among resistances do not necessarily hinder the use of MeJA, as clones that are constitutively more resistant to either pest, should show greater MeJA-induced resistance against the other.

Ecology, management and damage by the large pine weevil (Hylobius abietis) (Coleoptera: Curculionidae) in coniferous forests within Europe

Coniferous forests in Europe have a considerable number of pests that attack trees of all ages from youngest up to oldest ones. One of them is the large pine weevil Hylobius abietis. This species is widespread in Europe and occurs from warm southern areas (Spain) up to northern countries (Sweden, Finland). Larvae of this species do not cause damage, they help in the decomposition process of stumps and roots. Adults are harmful as they consume bark of young coniferous seedlings, above all of spruce and pine. One adult can consume on average 75 mm2 of bark per day. Individuals can live up to 4 years, and during their lives they can damage and kill several tens of coniferous seedlings. Traditional chemical protection of seedlings against this pest is and will gradually be more and more restricted or forbidden. In this review, we describe this method as well as all other alternative methods including biological protection. We estimate that H. abietis causes annual damages in Europe of almost 120 million € and damages several tens of thousands of hectares of young forest stands.

Stranger in a strange land: genetic variation of native insect resistance biomarkers in UK Sitka spruce (Picea sitchensis [Bong.] Carr.)

Reforestation in the British Isles (UK and Ireland) has been dominated with the use of an exotic conifer tree species, Sitka spruce (Picea sitchensis [Bong.] Carr.). Sitka breeding in the UK was developed from a single provenance, the Haida Gwaii Islands (Canada), which is both well suited to the British climate and highly susceptible to the white pine weevil (Pissodes strobi L.) in its native range. We examined variation and heritability of insect resistance related traits and assessed potential trade-offs with tree growth in 50 full-sibling families and 13 clonally replicated genotypes growing in the UK. We measured bark levels of three terpenes (dehydroabietic acid, (+)-3-carene and terpinolene) shown to confer resistance to the white pine weevil in Sitka spruce’s native range, on the principle that these defence compounds may also contribute to pest resistance in the UK. We compared our results with published findings from the native range and also used individuals from a Haida Gwaii seed lot grown in the UK for comparison of terpene levels. Dehydroabietic acid content in the UK breeding population was similar to populations from resistant native populations, but (+)-3-carene and terpinolene levels were relatively low. Narrow sense heritability for dehydroabietic acid, (+)-3-carene and terpinolene was estimated as 0.20, 0.93 and 0.98, respectively from the full-sib data, and this evidence of genetic variance was supported by estimates of broad sense heritability from the smaller clonal study. Terpene content was found to be positively correlated to growth traits. The heritability estimates and genetic correlations indicate that selective breeding should be effective in raising levels in the UK breeding population of the three candidate terpenes implicated in weevil resistance. However, low levels observed indicate that other provenances from the native range may produce greater short-term improvements for two of the terpenes.

Forest management practices in reduction of damage caused by Pine weevil (Hylobius abietis L.) and Cervidae animals in newly planted Scots pine forests.

<p><strong>Key words</strong>: forest regeneration, Conniflex, Trico, mechanical site preparation</p><p>Forest regeneration is crucial stage in commercial forest management because actions during this stage impact future forest productivity and value. Scots pine is one of the main tree species in forestry of the northern part of Europe. Foresters have to overcome different risk factors during regeneration process and two of them are seedling damage by Pine weevil (<em>Hylobius abietis</em> L) and browsing by herbivores of <em>Cervidae</em> family. Pine weevil is one of the main forest pests in Europe that damage regenerated coniferous stands. Damage caused by this pest can produce 70% mortality of planted seedlings. Another significant risk factor is browsing. With increase of deer <em>(Cervidae)</em> population the damaged forest area also increases to the extent that foresters choose other tree species for regeneration. The mitigation of these risks is possible with appliance of forest management practices.  There are used different repelents against Cervidae animals.  Regarding Pine weevil, due to restrictions on insecticide use in EU, different protective coating materials have been developed, and several studies show that soil preparation before planting reduce amount of damaged Pine seedlings and increase possibility of successful regeneration.  We evaluated if and how forest management methods - soil preparation techniques (spot mounding, disc trenching) in combination with different plant protection products (Conniflex, Trico) - affects the degree of damage caused by pine weevil and <em>Cervidae</em> animals to Scots pine seedlings one and two years after outplanting in four forest stands in Latvia. The results from this study shows that application of Conniflex reduce number of damaged trees by 16.8% first and 20.3% second year after outplanting compared to untreated seedlings. Soil preparation has significant impact on reduction of pine weevil damage. The best results have been achieved by planting with Conniflex treated seedlings on spot mounds (2.4% damaged trees compared to 48.9% in unprepared soil without treatment). Repellent Trico do not provide additional protection against Pine weevil and do not have long term protection effect against browsing. Overall conclusion is that combination of soil preparation and use of appropriate coating material (Conniflex) can provide sufficient protection without usage of insecticides, and Trico has to be applied before winter season to achieve effect of protection.</p>

Spatio-temporal distribution of Hylobius abietis in Scots pine stands – implications for pest monitoring

The protection of reforested areas against the large pine weevil Hylobius abietis is one of the greatest forest management challenges in many European countries. No information exists on the spatial distribution of this pest, which is necessary to assess its abundance and, consequently, to precisely estimate its threat to reforested sites. The aim of this study was to assess the spatio-temporal changes in the number of H. abietis beetles in reforested clear-cut areas (reforestations) and neighbouring Pinus sylvestris stands, from which these beetles are assumed to migrate to the reforestations. In this two-year study, baited traps were used to catch H. abietis beetles in the reforestations and neighbouring stands. The results revealed significantly more beetles in the neighbouring old stands (61–69 years) than in the young stands (5–11 years). The distribution of the beetles in both the old and young stands was inversely dependent on the distance from the stand edge. The large pine weevil migration to the reforestations was most intense in mid-May and early June. In May, most beetles were collected along the edges of the reforestations; in June, their distribution was more regular; and in July, the beetles were more abundant in the central part of the reforestations. These results allow H. abietis monitoring improvement, which is an important element of integrated forest protection.

Comparison of the planting success and risks of pine weevil damage on mineral soil and drained peatland sites three years after planting

Over 20% of regeneration operations will be on drained peatland in the next decade in Finland. There are only a few studies comparing the planting success and the risk of pine weevil ( (L.) feeding damage on mineral soil and drained peatland. Thirty sites planted with Norway spruce ( (L.) H. Karst.) container seedlings in 2009 in Southern and Central Finland were inventoried three growing seasons after planting. Prediction models for the probability of survival, pine weevil damage and the presence of ground vegetation cover were done separately for peatland and mineral soil sites. The planting success was 17% lower on peatland sites (1379 surviving seedlings ha) than on mineral soil (1654 seedlings ha). The factors explaining the survival were the ground vegetation cover and type of the planting spot on the peatland, and the ground vegetation cover on mineral soil. On mineral soil, 76% of the planting spots were on cultivated mineral soil while on peatland only 28% of the seedlings were planted on similar spots. There were also fewer seedlings that were surrounded by dense ground vegetation on mineral soil (4%) than on peatland (14%). Pine weevil feeding damage did not differ significantly on peatland (23%) or mineral soil (18%). The more time there was from clear-cutting, the more the probability of pine weevil feeding damage was reduced on both soil classes. Additionally, cover vegetation in the vicinity of the seedlings increased on mineral soil. Cultivated planting spots, especially those covered by mineral soil, prevented pine weevil feeding and reduced the harmful effects of vegetation on the seedlings both on mineral soil and peatland.Hylobius abetisPicea abies–1–1