Ovarian transcriptomic analysis of black Muscovy duck at the early, peak and late egg-laying stages

AbstractBroodiness, one of the maternal behaviors and instincts for natural breeding in birds, is an interesting topic in reproductive biology. Broodiness in poultry is characterized by persistent nesting, usually associated with cessation of egg laying. The study of avian broodiness is essential for bird conservation breeding and commercial poultry industry. In this study, we examined the hypothalamus transcriptome of Muscovy duck in three reproductive stages, including egg-laying anaphase (LA), brooding prophase (BP) and brooding metaphase (BM). Differences in gene expression during the transition from egg-laying to broodiness were examined, and 155, 379, 292 differently expressed genes (DEGs) were obtained by pairwise comparisons of LA-vs-BP, LA-vs-BM and BP-vs-BM, respectively (fold change≥ 1.5, P < 0.05). Gene Ontology Term (GO) enrichment analysis suggested a possible role of oxidative stress in the hypothalamus might invoke reproductive costs that potentially change genes expression. KEGG analysis revealed glutamatergic synapse, dopaminergic synapse, serotonergic synapse and GABAergic synapse pathway were significantly enriched, and regulator genes were identified. Eight gene expression patterns were illustrated by trend analysis and further clustered into three clusters. Additional six hub genes were identified through combining trend analysis and protein-protein interaction (PPI) analysis. Our results suggested that the cyclical mechanisms of reproductive function conversion include effects of oxidative stress, biosynthesis of neurotransmitters or their receptors, and interactions between glucocorticoids and thyroid hormones and regulatory genes. These candidate genes and biological pathways may be used as targets for artificial manipulation and marker-assisted breeding in the reproductive behavior.

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PSIV-3 Late-Breaking: Transcriptomic analysis in the liver of aged brown-egg laying hens with different intensity of eggshell color.

Journal of Animal Science ◽

10.1093/jas/sky404.628 ◽

2018 ◽

Vol 96 (suppl_3) ◽

pp. 286-286

Author(s):

G Han ◽

D Kil ◽

D Goo ◽

G Park ◽

J Kim ◽

...

Keyword(s):

Laying Hens ◽

Egg Laying ◽

Transcriptomic Analysis ◽

Eggshell Color

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Transcriptomic analysis reveals the molecular mechanism of apoptosis induced by Muscovy duck reovirus

Genes & Genomics ◽

10.1007/s13258-017-0567-y ◽

2017 ◽

Vol 39 (11) ◽

pp. 1227-1235 ◽

Cited By ~ 4

Author(s):

Quanxi Wang ◽

Mengxi Liu ◽

Xiaoqin Yuan ◽

Chunyan Li ◽

Shiyi Chen ◽

...

Keyword(s):

Molecular Mechanism ◽

Transcriptomic Analysis ◽

Muscovy Duck ◽

Muscovy Duck Reovirus

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Egg-laying and brooding stage-specific hormonal response and transcriptional regulation in pituitary of Muscovy duck (Cairina moschata)

Poultry Science ◽

10.3382/ps/pez433 ◽

2019 ◽

Vol 98 (11) ◽

pp. 5287-5296

Author(s):

Pengfei Ye ◽

Kai Ge ◽

Min Li ◽

Lei Yang ◽

Sihua Jin ◽

...

Keyword(s):

Transcriptional Regulation ◽

Egg Laying ◽

Muscovy Duck ◽

Hormonal Response ◽

Cairina Moschata

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Associations of IGF2 and DRD2 polymorphisms with laying traits in Muscovy duck

PeerJ ◽

10.7717/peerj.4083 ◽

2017 ◽

Vol 5 ◽

pp. e4083 ◽

Cited By ~ 3

Author(s):

Qiao Ye ◽

Jiguo Xu ◽

Xinfeng Gao ◽

Hongjia Ouyang ◽

Wei Luo ◽

...

Keyword(s):

Growth Factor ◽

Egg Production ◽

Follicular Development ◽

Egg Laying ◽

Muscovy Duck ◽

Nucleotide Polymorphisms ◽

Production Traits ◽

Specific Expression ◽

Single Nucleotide ◽

Muscovy Ducks

Insulin-like growth factor 2 (IGF2) and dopamine receptor 2 (DRD2) play important roles in ovarian follicular development. In this study, we analyzed tissue-specific expression of the Muscovy duck IGF2 and DRD2 genes and cloned those genes transcripts. Polymorphisms in these genes were tightly linked with egg production traits and both genes were highly expressed in the ovary. Moreover, we identified five single nucleotide polymorphisms (SNPs) for IGF1 and 28 for DRD2. Mutations A-1864G and C-1704G of IGF2 were positively correlated with increased egg laying at 59 weeks (E59W) (P < 0.05). The C+7T and C+364G mutations of DRD2 were highly and significantly associated with first-egg age (FEA) and egg numbers at 300 days (E300D) (P < 0.01). Moreover, C+3301G and C+3545G of DRD2 were highly significantly associated with FEA, E59W and E300D (P < 0.01). Other mutations were positively associated with FEA or E300D or E59W (P < 0.05). These data suggest specific roles for IGF1 and DRD2 polymorphisms in egg production in Muscovy ducks.

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Investigation of insulin-like growth factor-1 gene with egg-laying traits in the Muscovy duck (Cairina moschata)

Canadian Journal of Animal Science ◽

10.1139/cjas-2015-0024 ◽

2016 ◽

Vol 96 (2) ◽

pp. 203-214 ◽

Cited By ~ 4

Author(s):

X. Wu ◽

X.T. Liu ◽

J.J. Lan ◽

X.P. Wan ◽

M.J. Yan ◽

...

Keyword(s):

Growth Factor ◽

Marker Gene ◽

Gonad Development ◽

Egg Laying ◽

Primary Concern ◽

Muscovy Duck ◽

Insulin Like Growth Factor ◽

Expression Levels ◽

Cairina Moschata ◽

First Time

Muscovy duck (Cairina moschata) is characterized by broodiness, and egg-laying traits in this species should be of primary concern. As a member of the IGF superfamily of proteins, insulin-like growth factor-1 (IGF-1) is an important positive regulator of growth and gonad development in vertebrates. To explore the effect of IGF-1 on the growth hormone/insulin-like growth factors (GH/IGF) axis, we cloned and characterized IGF-1 of Muscovy duck. The expression level of IGF-1 was widely expressed in all the tested tissues, and the highest level was detected in the liver. In laying ducks, IGF-1 expression levels in the hypothalamus, pituitary, and ovary were very significantly higher (P < 0.01) than that in the tissues of nesting duck, and IGF-1 expression levels in the heart and liver were significantly higher (P < 0.05) than that in nesting duck tissues. Furthermore, a single nucleotide polymorphism (SNP) (A/G) was found and significantly (P < 0.05) associated with age at first egg and egg number at 300 d. This study provides the first evidence that IGF-1 promote egg-laying traits of Muscovy duck through two axes, involving GH/IGF and hypothalamic–pituitary–gonadal axes. These findings enrich the information of IGF-1 of Muscovy duck and demonstrate for the first time the ability of IGF-1 to promote reproduction, indicating that IGF-1 could be used as an important marker gene.

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Erysipelas in egg-laying chickens: Clinical, pathological and bacteriological investigations

Avian Pathology ◽

10.1080/03079457508418133 ◽

1975 ◽

Vol 4 (1) ◽

pp. 59-71 ◽

Cited By ~ 1

Author(s):

M. Bisgaard ◽

P. Olsen

Keyword(s):

Egg Laying

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Experimental increase in female mass during the fertile phase leads to enhanced extra-pair paternity in pied flycatchers Ficedula hypoleuca

10.32942/osf.io/qrnkb ◽

2019 ◽

Author(s):

Mireia Plaza ◽

Alejandro Cantarero ◽

Juan Moreno

Keyword(s):

Negative Relationship ◽

Ficedula Hypoleuca ◽

Egg Laying ◽

Female Dominance ◽

Laying Date ◽

Control Group ◽

Wing Loading ◽

Potential Fecundity ◽

Fertile Phase ◽

Extra Pair Paternity

Female mass in most altricial birds reaches its maximum during breeding at egg-laying, which coincides temporally with the fertile phase when extra-pair paternity (EPP) is determined. Higher mass at laying may have two different effects on EPP intensity. On the one hand, it would lead to increased wing loading (body mass/wing area), which may impair flight efficiency and thereby reduce female’s capacity to resist unwanted extra-pair male approaches (sexual conflict hypothesis). On the other hand, it would enhance female condition, favouring her capacity to evade mate-guarding and to search for extra-pair mates (female choice hypothesis). In both cases, higher female mass at laying may lead to enhanced EPP. To test this prediction, we reduced nest building effort by adding a completely constructed nest in an experimental group of female pied flycatchers (Ficedula hypoleuca). Our treatment caused an increase in mass and thereby wing loading and this was translated into a significantly higher EPP in the manipulated group compared with the control group as expected. There was also a significant negative relationship between EPP and laying date and the extent of the white wing patch, an index of female dominance. More body reserves at laying mean not only a higher potential fecundity but a higher level of EPP as well. This interaction had not previously received due attention but should be considered in future studies of avian breeding strategies.

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