Expression of Hypoxia-Inducible Factor-1α and Myoglobin in Rat Heart as Adaptive Response to Intermittent Hypobaric Hypoxia Exposure

This study tested the hypothesis that athletes exposed to 4 wk of intermittent hypobaric hypoxia exposure (3 h/day, 5 days/wk at 4,000–5,500 m) or double-blind placebo increase their red blood cell volume (RCV) and hemoglobin mass (Hbmass) secondary to an increase in erythropoietin (EPO). Twenty-three collegiate level athletes were measured before (Pre) and after (Post) the intervention for RCV via Evans blue (EB) dye and in duplicate for Hbmassusing CO rebreathing. Hematological indexes including EPO, soluble transferrin receptor, and reticulocyte parameters were measured on 8–10 occasions spanning the intervention. The subjects were randomly divided among hypobaric hypoxia (Hypo, n = 11) and normoxic (Norm, n = 12) groups. Apart from doubling EPO concentration 3 h after hypoxia there was no increase in any of the measures for either Hypo or Norm groups. The mean change in RCV from Pre to Post for the Hypo group was 2.3% (95% confidence limits = −4.8 to 9.5%) and for the Norm group was −0.2% (−5.7 to 5.3%). The corresponding changes in Hbmasswere 1.0% (−1.3 to 3.3%) for Hypo and −0.3% (−2.6 to 3.1%) for Norm. There was good agreement between blood volume (BV) from EB and CO: EB BV = 1.03 × CO BV + 142, r2= 0.85, P < 0.0001. Overall, evidence from four independent techniques (RCV, Hbmass, reticulocyte parameters, and soluble transferrin receptor) suggests that INTERMITTENT HYPOBARIC HYPOXIA EXPOSURE did not accelerate erythropoiesis despite the increase in serum EPO.

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Blood rheological behaviour in rats after intermittent hypobaric hypoxia exposure to 5000 m

Comparative Biochemistry and Physiology Part A Molecular & Integrative Physiology ◽

10.1016/j.cbpa.2007.01.340 ◽

2007 ◽

Vol 146 (4) ◽

pp. S164

Author(s):

S. Esteva ◽

P. Panisello ◽

R. Torrella ◽

T. Pagès ◽

G. Viscor

Keyword(s):

Hypobaric Hypoxia ◽

Rheological Behaviour ◽

Hypoxia Exposure ◽

Intermittent Hypobaric Hypoxia

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Evaluating hypoxia-inducible factor-1α mRNA expression in a pelagic fish, Pacific herring Clupea pallasii, as a biomarker for hypoxia exposure

Comparative Biochemistry and Physiology Part A Molecular & Integrative Physiology ◽

10.1016/j.cbpa.2015.07.016 ◽

2015 ◽

Vol 189 ◽

pp. 58-66 ◽

Cited By ~ 14

Author(s):

Halley E. Froehlich ◽

Steven B. Roberts ◽

Timothy E. Essington

Keyword(s):

Mrna Expression ◽

Hypoxia Inducible Factor ◽

Pelagic Fish ◽

Pacific Herring ◽

Clupea Pallasii ◽

Hypoxia Inducible Factor 1Α ◽

Hypoxia Exposure

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Assessment of hypoxia-inducible factor-1α mRNA expression in mantis shrimp as a biomarker of environmental hypoxia exposure

Biology Letters ◽

10.1098/rsbl.2011.0887 ◽

2011 ◽

Vol 8 (2) ◽

pp. 278-281 ◽

Cited By ~ 20

Author(s):

Keita Kodama ◽

Md. Saydur Rahman ◽

Toshihiro Horiguchi ◽

Peter Thomas

Keyword(s):

Hypoxia Inducible Factor ◽

Cerebral Ganglion ◽

Ecological Impacts ◽

Tokyo Bay ◽

Mrna Levels ◽

Transcript Levels ◽

Mantis Shrimp ◽

Hypoxia Inducible Factor 1Α ◽

Hypoxia Exposure ◽

Environmental Hypoxia

Efforts to assess the ecological impacts of the marked increase in coastal hypoxia worldwide have been hampered by a lack of biomarkers of hypoxia exposure in marine benthic organisms. Here, we show that hypoxia-inducible factor-1α (HIF-1α) transcript levels in the heart and cerebral ganglion of mantis shrimp ( Oratosquilla oratoria ) collected from hypoxic sites in Tokyo Bay are elevated several-fold over those in shrimp collected from normoxic sites. Upregulation of HIF-1α mRNA levels in the heart after exposure to sub-lethal hypoxia was confirmed in controlled laboratory experiments. HIF-1α transcript levels were increased at approximately threefold after 7 and 14 days of hypoxia exposure and declined to control levels within 24 h of restoration to normoxic conditions. The results provide the first evidence for upregulation of HIF-1α transcript levels in two hypoxia-sensitive organs, heart and cerebral ganglion, in a marine invertebrate exposed to environmental hypoxia. These results suggest that upregulation of HIF-1α transcript levels is an important component in adaptation of mantis shrimp to chronic hypoxia and is a potentially useful biomarker of environmental hypoxia exposure.

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The Effect of Intermittent Hypobaric Hypoxia Exposure on Reduced-Glutathione Level in Rat Lung and Renal Tissues

Advanced Science Letters ◽

10.1166/asl.2018.12704 ◽

2018 ◽

Vol 24 (8) ◽

pp. 6249-6251

Author(s):

N. S Hardiany ◽

A. A Asa ◽

D Safirina ◽

W Mulyawan

Keyword(s):

Hypobaric Hypoxia ◽

Reduced Glutathione ◽

Renal Tissue ◽

Rat Lung ◽

Sprague Dawley ◽

Hypoxia Exposure ◽

Intermittent Hypobaric Hypoxia ◽

Sprague Dawley Rats ◽

Adaptation Response ◽

Ellman’S Method

Hypobaric hypoxia is basically a hypoxia condition experienced in high altitude commonly during flight, that increase reactive oxygen species (ROS). When hypoxia hypobaric does not undergo continuation or in other word, intermittent, it will cause adaptation response in a form of protection mode into ROS. Moreover, ROS could be eliminated by reduced-glutathione (GSH) as an endogenous non enzymatic antioxidant. Therefore, the aim of this study was to analyze the effects of intermittent hypobaric hypoxia exposure on GSH level in rat lung and renal tissue. Lung and renal samples were collected from 6–8 weeks old male Sprague-Dawley rats weighing 150–200 g, previously exposed 1–4 times to intermittent hypobaric hypoxia in 35,000 ft (1 minute), 25.000 ft (5 minute) and 18,000 ft altitude (25 minute). Afterwards, GSH level was calculated from lung and renal extracts using the Ellman’s method. In lung tissues, GSH level was decreased in hypoxia 1×, 2×, 3×, 4× treatment, and were significant between the control–hypoxia 3×, control–hypoxia 4×, hypoxia 1×–hypoxia 3× and hypoxia 1×–hypoxia 4×. On the contrary, GSH level was increased in renal tissues on hypoxia 1× and hypoxia 2× treatment compared to control. Nevertheless, GSH level was decreased after 3× treatment and found almost stabilized at 4× treatment of hypoxia in renal tissues. Intermittent hypobaric hypoxia exposure affect GSH in rat lung and renal tissues with varying level as an adaptive response system.

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