In-situ lipopeptide biosurfactant production by Bacillus strains correlates with improved oil recovery in two oil wells approaching their economic limit of production

2013 ◽  
Vol 81 ◽  
pp. 127-132 ◽  
Author(s):  
Noha Youssef ◽  
D. Randall Simpson ◽  
Michael J. McInerney ◽  
Kathleen E. Duncan
Keyword(s):  
Oil Recovery ◽  
Oil Wells ◽  
Biosurfactant Production ◽  
Improved Oil Recovery ◽  
Lipopeptide Biosurfactant ◽  
Bacillus Strains

scholarly journals Anaerobic lipopeptide biosurfactant production by an engineered bacterial strain for in situ microbial enhanced oil recovery

RSC Advances ◽  
2017 ◽  
Vol 7 (33) ◽  
pp. 20667-20676 ◽  
Author(s):  
Xiaolong Liang ◽  
Rongjiu Shi ◽  
Mark Radosevich ◽  
Feng Zhao ◽  
Yingyue Zhang ◽  
...  
Keyword(s):  
Enhanced Oil Recovery ◽  
Oil Recovery ◽  
Bacterial Strain ◽  
Potential Application ◽  
Biosurfactant Production ◽  
Microbial Enhanced Oil Recovery ◽  
Lipopeptide Biosurfactant

Anaerobic lipopeptide biosurfactant production by engineered bacterial strain FA-2 was fulfilled forin situMEOR potential application.

scholarly journals In Situ Biosurfactant Production by Bacillus Strains Injected into a Limestone Petroleum Reservoir

2006 ◽  
Vol 73 (4) ◽  
pp. 1239-1247 ◽  
Author(s):  
N. Youssef ◽  
D. R. Simpson ◽  
K. E. Duncan ◽  
M. J. McInerney ◽  
M. Folmsbee ◽  
...  
Keyword(s):  
Oil Recovery ◽  
Molecular Data ◽  
Average Concentration ◽  
Biosurfactant Production ◽  
Petroleum Reservoir ◽  
Minimum Concentration ◽  
Recovery Processes ◽  
Microbial Oil Recovery ◽  
Carbon Balances

ABSTRACT Biosurfactant-mediated oil recovery may be an economic approach for recovery of significant amounts of oil entrapped in reservoirs, but evidence that biosurfactants can be produced in situ at concentrations needed to mobilize oil is lacking. We tested whether two Bacillus strains that produce lipopeptide biosurfactants can metabolize and produce their biosurfactants in an oil reservoir. Five wells that produce from the same Viola limestone formation were used. Two wells received an inoculum (a mixture of Bacillus strain RS-1 and Bacillus subtilis subsp. spizizenii NRRL B-23049) and nutrients (glucose, sodium nitrate, and trace metals), two wells received just nutrients, and one well received only formation water. Results showed in situ metabolism and biosurfactant production. The average concentration of lipopeptide biosurfactant in the produced fluids of the inoculated wells was about 90 mg/liter. This concentration is approximately nine times the minimum concentration required to mobilize entrapped oil from sandstone cores. Carbon dioxide, acetate, lactate, ethanol, and 2,3-butanediol were detected in the produced fluids of the inoculated wells. Only CO2 and ethanol were detected in the produced fluids of the nutrient-only-treated wells. Microbiological and molecular data showed that the microorganisms injected into the formation were retrieved in the produced fluids of the inoculated wells. We provide essential data for modeling microbial oil recovery processes in situ, including growth rates (0.06 � 0.01 h−1), carbon balances (107% � 34%), biosurfactant production rates (0.02 � 0.001 h−1), and biosurfactant yields (0.015 � 0.001 mol biosurfactant/mol glucose). The data demonstrate the technical feasibility of microbial processes for oil recovery.

Improved Oil Recovery through Cost Effective In-Situ Gas Injection - A Case Study of Badin

2017 ◽  
Author(s):  
Muhammad Noman ◽  
Faisal Mehmood ◽  
Waqar M. Ghouri
Keyword(s):  
Oil Recovery ◽  
Gas Injection ◽  
Cost Effective ◽  
Improved Oil Recovery

scholarly journals Development of an In Situ Biosurfactant Production Technology for Enhanced Oil Recovery

2007 ◽  
Author(s):  
M.J. McInerney ◽  
R.M. Knapp ◽  
Kathleen Duncan ◽  
D.R. Simpson ◽  
N. Youssef ◽  
...  
Keyword(s):  
Enhanced Oil Recovery ◽  
Production Technology ◽  
Oil Recovery ◽  
Biosurfactant Production
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