A rhodamine B-based turn on fluorescent probe for selective recognition of mercury(II) ions

2021 ◽  
Vol 520 ◽  
pp. 120285
Author(s):  
Chenfang Cui ◽  
Xu Gao ◽  
Xianchao Jia ◽  
Yang Jiao ◽  
Chunying Duan
Keyword(s):  
Fluorescent Probe ◽  
Rhodamine B ◽  
Selective Recognition ◽  
Turn On

Colorimetric and Fluorescent Probe for Hypochlorite with High Selectivity

2013 ◽  
Vol 295-298 ◽  
pp. 475-478 ◽  
Author(s):  
Zhi Xiang Han ◽  
Ming Hui Du ◽  
Guo Xi Liang ◽  
Xiang Yang Wu
Keyword(s):  
Reactive Oxygen Species ◽  
Aqueous Solution ◽  
Detection Limit ◽  
Fluorescent Probe ◽  
Rhodamine B ◽  
High Selectivity ◽  
Reactive Oxygen ◽  
Living Cells ◽  
Oxygen Species ◽  
Turn On

Rhodamine B thiohydrazide (RBS) was firstly employed as turn-on fluorescent probe for hypochlorite in aqueous solution and living cells. It exhibits a stable response to hypochlorite from 1.0×10-6to 1.0×10-5M with a detection limit of 3.3×10-7M. The response of this probe to hypochlorite is fast and highly selective compared with other reactive oxygen species (such as.OH,1O2, H2O2) and other common anions (such as X-, ClO2-, ClO4-, NO3-, NO2-, OH-, Ac-, CO32-, SO42-).

Highly sensitive and selective turn-on fluorescent probes for Cu2+ based on rhodamine B

2015 ◽  
Vol 3 (26) ◽  
pp. 5261-5265 ◽  
Author(s):  
Ye Yuan ◽  
Shiguo Sun ◽  
Si Liu ◽  
Xinwei Song ◽  
Xiaojun Peng
Keyword(s):  
Fluorescent Probe ◽  
Fluorescent Probes ◽  
Rhodamine B ◽  
Turn On ◽  
Highly Sensitive

A new rhodamine B-based fluorescent probe RL for Cu2+ has been designed, synthesized, and characterized.

scholarly journals Development of tBu-phenyl Acetamide Appended Thiacalix[4]arene as “Turn-ON” Fluorescent Probe for Selective Recognition of Hg(II) Ions

2022 ◽  
Author(s):  
Ashukumar Verma ◽  
Krunal Modi ◽  
Shuvankar Dey ◽  
Anita Kongor ◽  
Manthan Panchal ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Selective Recognition ◽  
Turn On

Oxo-spirocyclic structure bridged ditopic Schiff base: A turn-on fluorescent probe for selective recognition of Zn(II) and its application in biosensing

2018 ◽  
Vol 149 ◽  
pp. 921-926 ◽  
Author(s):  
Zheng-Yi Li ◽  
Hong-Kui Su ◽  
Kun Zhou ◽  
Bao-Zhu Yang ◽  
Tangxin Xiao ◽  
...  
Keyword(s):  
Schiff Base ◽  
Fluorescent Probe ◽  
Selective Recognition ◽  
Turn On

A highly specific rhodamine B based turn-on fluorescent probe for nitric oxide and application in living cells

Tetrahedron ◽  
2020 ◽  
Vol 76 (46) ◽  
pp. 131622
Author(s):  
Yuanyuan Liu ◽  
Chunpeng Jiao ◽  
Yongchun Wei ◽  
Wenjuan Lu ◽  
Pingping Zhang ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Fluorescent Probe ◽  
Rhodamine B ◽  
Living Cells ◽  
Turn On

A turn-on fluorescent probe for Lu3+ recognition and bio-imaging in live cells and zebrafish

2021 ◽  
Author(s):  
Mujthaba Aatif A ◽  
Selva Kumar R ◽  
S. Abdul Majeed ◽  
S. K. Ashok Kumar
Keyword(s):  
Fluorescent Probe ◽  
High Performance ◽  
Selective Recognition ◽  
Live Cells ◽  
Turn On ◽  
Zebrafish Larvae ◽  
Bio Imaging

A high-performance fluorescent probe (L) for selective recognition of Lu3+ is developed. The probe L selectively recognizes Lu3+via CHEF and it can detect Lu3+ as low as 23 nM. The probe L applied in bioimaging of Lu3+ in zebrafish larvae.

A Boronic Acid-Based Fluorescence Hydrogel for Monosaccharide Detection

2018 ◽  
Author(s):  
Suying Xu ◽  
Adam Sedgwick ◽  
Souad Elfecky ◽  
Wenbo Chen ◽  
Ashley Jones ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Boronic Acid ◽  
Binding Constants ◽  
Strong Fluorescence ◽  
Fluorescence Response ◽  
Turn On ◽  
Fluorescence Turn On ◽  
Hydrogel System

<p>A boronic acid-based anthracene fluorescent probe was functionalised with an acrylamide unit to incorporate into a hydrogel system for monosaccharide detection<i>. </i>In solution, the fluorescent probe<b> </b>displayed a strong fluorescence turn-on response upon exposure to fructose, and an expected trend in apparent binding constants, as judged by a fluorescence response where D-fructose > D-galactose > D-mannose > D-glucose. The hydrogel incorporating the boronic acid monomer demonstrated the ability to detect monosaccharides by fluorescence with the same overall trend as the monomer in solution with the addition of fructose resulting in a 10-fold enhancement (≤ 0.25 M). <b><u></u></b></p>

Synthesis and Characterization of ROSA Dye - A Rhodamine B-type Fluorophore, Suitable for Bioconjugation and Fluorescence Studies in Live Cells

2019 ◽  
Vol 26 (10) ◽  
pp. 758-767
Author(s):  
Vicente Rubio ◽  
Vijaya Iragavarapu ◽  
Maciej J. Stawikowski
Keyword(s):  
Quantum Yield ◽  
Cancer Cells ◽  
Fluorescent Probe ◽  
Extinction Coefficient ◽  
Rhodamine B ◽  
Solid Phase ◽  
Total Yield ◽  
Molar Extinction Coefficient ◽  
Αvβ3 Integrin ◽  
Αvβ3 Integrins

Background: Herein we report the multigram-scale synthesis, characterization and application of a rhodamine B-based fluorophore (ROSA) suitable for fluorescent studies in biological applications. This fluorophore is devoid of rhodamine spirolactone formation and furthermore characterized by a high molar extinction coefficient (ϵ=87250 ± 1630 M-1cm-1) and quantum yield (φ) of 0.589 ± 0.070 in water. Reported here is also the application of ROSA towards synthesis of a ROSA-PEG-GRGDS-NH2 fluorescent probe suitable for live cell imaging of αvβ3 integrins for in vitro assays. Objective: The main objective of this study is to efficiently prepare rhodamine B derivative, devoid of spirolactone formation that would be suitable for bioconjugation and subsequent bioimaging. Methods: Rhodamine B was transformed into rhodamine B succinimide ester (RhoB-OSu) using N-hydroxysuccinimide. RhoB-OSu was further coupled to sarcosine to obtain rhodamine Bsarcosine dye (ROSA) in good yield. The ROSA dye was then coupled to a αvβ3 integrin binding sequence using standard solid-phase conditions. Resulting ROSA-PEG-GRGDS-NH2 probe was used to image integrins on cancer cells. Results: The rhodamine B-sarcosine dye (ROSA) was obtained in multigram scale in good total yield of 47%. Unlike rhodamine B, the ROSA dye does not undergo pH-dependent spirolactone/spirolactam formation as compared with rhodamine B-glycine. It is also characterized by excellent quantum yield (φ) of 0.589 ± 0.070 in water and high molar extinction coefficient of 87250 ± 1630 M-1cm-1. ROSA coupling to the RGD-like peptide was proved to be efficient and straightforward. Imaging using standard filters on multimode plate reader and confocal microscope was performed. The αvβ3 integrins present on the surface of live WM-266-4 (melanoma) and MCF- 7 (breast cancer) cells were successfully imaged. Conclusion: We successfully derivatized rhodamine B to create an inexpensive, stable and convenient to use fluorescent probe. The obtained derivative has excellent photochemical properties and it is suitable for bioconjugation and many imaging applications.

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