Reduction of E. coli O157: H7 and Bacillus cereus levels in red pepper powder using dielectric barrier discharge (DBD) plasma for enhanced quality

Author(s):  
Yeon Ho Kim ◽  
Chanjae Lee ◽  
Sung-Jae Lee ◽  
Ki Sun Yoon
Foods ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 171 ◽  
Author(s):  
Eun Bi Jeon ◽  
Man-Seok Choi ◽  
Ji Yoon Kim ◽  
Shin Young Park

The synergistic efficacy of a combined treatment of mild heat (MH) and dielectric barrier discharge (DBD) plasma in Bacillus cereus-contaminated red pepper powder was tested. A cocktail of three strains of B. cereus (NCCP 10623, NCCP 14579, ATCC 11778) was inoculated onto red pepper powder and then treated with MH (60 °C for 5–20 min) and DBD plasma (5–20 min). Treatment with MH and DBD plasma alone for 5–20 min resulted in reductions of 0.23–1.43 and 0.12–0.96 log CFU/g, respectively. Combined treatment with MH and DBD plasma was the most effective at reducing B. cereus counts on red pepper powder, and resulted in log-reductions of ≥6.0 log CFU/g. The largest synergistic values (4.24–4.42 log) against B. cereus in red pepper powder were obtained by the combination of 20 min MH and 5–15 min DBD plasma. The values of Hunter color ‘‘L’’, ‘‘a’’, and ‘‘b’’, were not significantly different from those of the untreated sample and that with the combination of MH (60 °C for 5–20 min) and DBD plasma (5–20 min). Also, no significant (p > 0.05) differences in pH values between samples were observed. Therefore, these results suggest that the combination of MH treatment and DBD plasma can be potentially utilized in the food industry to effectively inactivate B. cereus without incurring quality deterioration of red pepper powder.


Author(s):  
Eun Bi Jeon ◽  
Man-Seok Choi ◽  
Ji Yoon Kim ◽  
Shin Young Park

The synergistic efficacy of combined treatment mild heat (MH) and dielectric barrier discharge (DBD) plasma in Bacillus cereus-contaminated red pepper powder was tested. A cocktail of three strains of B. cereus (NCCP 10623, NCCP 14579, ATCC 11778) was inoculated onto red pepper powder and then treated with MH (60 ℃ for 5-20 min) and DBD plasma (5-20 min). Treatment with MH and DBD plasma alone for 5~20 min resulted in reductions of 0.23~1.43 and 0.12~0.96 log CFU/g, respectively. Combined treatment with MH and DBD plasma was the most effective at reducing B. cereus counts on red pepper powder and resulted in log-reductions of ≥ 6.0 log CFU/g. The largest synergistic values (4.24-4.42 log) against B. cereus in red pepper powder were obtained by the combination of 20 min MH and 5~15 min DBD plasma. Hunter color ‘‘L’’, ‘‘a’’, and ‘‘b’’ values of the combination-treated samples were not significantly different from those of non-treated samples. Also, no significant (p > 0.05) differences in pH values between samples were observed. Therefore, these results suggest that the combination of MH treatment and DBD plasma can be potentially utilized in the food industry to effectively inactivate B. cereus without incurring quality deterioration of red pepper powder.


Foods ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 1013 ◽  
Author(s):  
Ji Yoon Kim ◽  
Eun Bi Jeon ◽  
Man-Seok Choi ◽  
Eun Ha Choi ◽  
Jun Sup Lim ◽  
...  

This study investigated the effects of atmospheric dielectric barrier discharge (DBD) plasma (1.1 kV, 43 kHz, 5–30 min, N2: 1.5 L/m) on the reduction of Escherichia coli and Bacillus cereus on dried laver. The reductions of E. coli and B. cereus by 5, 10, 20, and 30 min of DBD plasma were 0.56 and 0.24, 0.61 and 0.66, 0.76 and 1.24, and 1.02 and 1.38 log CFU/g, respectively. The D-value of E. coli and B. cereus was predicted as 29.80 and 20.53 min, respectively, using the Weibull model for E. coli (R2 = 0.95) and first-order kinetics for B. cereus (R2 = 0.94). After DBD plasma 5–30 min treatment, there was no change in pH (6.20–6.21) and this value was higher than the untreated dried laver (6.08). All sensory scores in DBD plasma-treated laver were determined as >6 points. The 30 min of DBD plasma is regarded as a novel intervention for the control of potential hazardous bacteria in dried laver.


Nanomaterials ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 961 ◽  
Author(s):  
Zhiyuan Fan ◽  
Lanbo Di ◽  
Xiuling Zhang ◽  
Hongyang Wang

Cotton-fabric-supported silver nanoparticles (Ag NPs) have aroused great attention due to their remarkable physical and chemical properties and excellent broad-spectrum antibacterial performance.In this work, a surface dielectric barrier discharge (DBD) plasma method is developed and employed to prepare cotton fabric supported Ag NPs (Ag/cotton) for the first time. UV-Vis and X-ray photoelectron spectroscopy (XPS) results confirm the formation of Ag NPs. TEM images show that the size of Ag NPs is in the range 4.8–5.3 nm. Heat-sensitive cotton fabrics are not destroyed by surface DBD plasma according to FTIR and XRDresults. Wash fastness of the Ag/cotton samples is investigated using ultrasonic treatment for 30 min and it is shown that the Ag NPs possess good adhesion to the cotton fabric according to UV-Vis spectra. Antibacterial activity of the Ag/cotton samples shows that obvious bacteriostasis loops are observed around the samples with the appearance of both Gram-negative bacterium Escherichia coli (E. coli) and Gram-positive bacterium Bacillus subtilis (B. subtilis). The average diameter of the bacteriostasis loops against both E. coli and B. subtilis becomes larger with an increasing silver loading amount.This work provides a universal, fast, simple, and environmentally-friendly cold plasma method for synthesizing Ag NPs on heat-sensitive materials at atmospheric pressure.


Plasma ◽  
2018 ◽  
Vol 1 (1) ◽  
pp. 144-149 ◽  
Author(s):  
Yingying Li ◽  
Rida Atif ◽  
Ketao Chen ◽  
Jiushan Cheng ◽  
Qiang Chen ◽  
...  

Dielectric barrier discharge (DBD) plasma has been found to uniquely polymerize ribose that is not usually subject to polymerization since molecules that tend to polymerize almost always possess at least a π-bond. The polymer was analyzed via nuclear magnetic resonance (NMR) spectra, matrix-assisted laser desorption ionization time-of-flight (MALDI TOF) mass spectroscopy and Fourier-Transform inferred spectroscopy (FTIR), and it was found that dehydration occurs during polymerization.


Author(s):  
Yi Chen ◽  
Li Fei ◽  
Liming He ◽  
Lei Zhang ◽  
Chunchang Zhu ◽  
...  

A test platform was developed to investigate the performance of aero-engine combustor by the dielectric barrier discharge (DBD) plasma assisted combustion (PAC) in the simulated maximum condition. Conventional combustion experiments and plasma-assisted combustion conditions were conducted to study the effect of PAC on the performances including average outlet temperature, combustion efficiency and pattern factor under four different excessive air coefficients five different voltages. The comparative experiment shows that the combustion efficiency is improved after PAC compared with the normal conditions, the combustion efficiency of PAC increases 2.31% in the fuel-rich condition when Up-p is 40 kV. The uniformity of the outlet temperature field is also improved after PAC, the decrease of the pattern factor is more than 5% in the fuel-rich condition. These results offer certain reference value for the future application of PAC in aero-engine combustor and improving its performance.


Sign in / Sign up

Export Citation Format

Share Document