Treatment of water and E. Coli suspensions by dielectric barrier discharge in argon/oxygen atmospheres

2011 ◽  
Author(s):  
K. Oehmigen ◽  
J. Winter ◽  
Ch. Wilke ◽  
K.-D. Weltmann ◽  
Th. von Woedtke
Keyword(s):  
Dielectric Barrier Discharge ◽  
Barrier Discharge ◽  
Dielectric Barrier ◽  
E Coli

scholarly journals Antibiotic-Resistant and Non-Resistant Bacteria Display Similar Susceptibility to Dielectric Barrier Discharge Plasma

2020 ◽  
Vol 21 (17) ◽  
pp. 6326
Author(s):  
Akikazu Sakudo ◽  
Tatsuya Misawa
Keyword(s):  
Plasma Treatment ◽  
Cell Count ◽  
Dielectric Barrier Discharge ◽  
Barrier Discharge ◽  
Viable Cell ◽  
Resistant Bacteria ◽  
Free Medium ◽  
Antibiotic Resistant ◽  
Dielectric Barrier ◽  
E Coli

Here, we examined whether antibiotic-resistant and non-resistant bacteria show a differential susceptibility to plasma treatment. Escherichia coli DH5α were transformed with pPRO-EX-HT-CAT, which encodes an ampicillin resistance gene and chloramphenicol acetyltransferase (CAT) gene, and then treated with a dielectric barrier discharge (DBD) plasma torch. Plasma treatment reduced the viable cell count of E. coli after transformation/selection and further cultured in ampicillin-containing and ampicillin-free medium. However, there was no significant difference in viable cell count between the transformed and untransformed E. coli after 1 min- and 2 min-plasma treatment. Furthermore, the enzyme-linked immunosorbent assay (ELISA) and acetyltransferase activity assay showed that the CAT activity was reduced after plasma treatment in both transformed and selected E. coli grown in ampicillin-containing or ampicillin-free medium. Loss of lipopolysaccharide and DNA damage caused by plasma treatment were confirmed by a Limulus test and polymerase chain reaction, respectively. Taken together, these findings suggest the plasma acts to degrade components of the bacteria and is therefore unlikely to display a differential affect against antibiotic-resistant and non-resistant bacteria. Therefore, the plasma method may be useful in eliminating bacteria that are recalcitrant to conventional antibiotic therapy.

scholarly journals A Surface Dielectric Barrier Discharge Plasma for Preparing Cotton-Fabric-Supported Silver Nanoparticles

Nanomaterials ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 961 ◽  
Author(s):  
Zhiyuan Fan ◽  
Lanbo Di ◽  
Xiuling Zhang ◽  
Hongyang Wang
Keyword(s):  
Silver Nanoparticles ◽  
Dielectric Barrier Discharge ◽  
Cotton Fabric ◽  
Barrier Discharge ◽  
Dbd Plasma ◽  
Ag Nps ◽  
Dielectric Barrier ◽  
E Coli ◽  
Plasma Method ◽  
Surface Dielectric Barrier Discharge

Cotton-fabric-supported silver nanoparticles (Ag NPs) have aroused great attention due to their remarkable physical and chemical properties and excellent broad-spectrum antibacterial performance.In this work, a surface dielectric barrier discharge (DBD) plasma method is developed and employed to prepare cotton fabric supported Ag NPs (Ag/cotton) for the first time. UV-Vis and X-ray photoelectron spectroscopy (XPS) results confirm the formation of Ag NPs. TEM images show that the size of Ag NPs is in the range 4.8–5.3 nm. Heat-sensitive cotton fabrics are not destroyed by surface DBD plasma according to FTIR and XRDresults. Wash fastness of the Ag/cotton samples is investigated using ultrasonic treatment for 30 min and it is shown that the Ag NPs possess good adhesion to the cotton fabric according to UV-Vis spectra. Antibacterial activity of the Ag/cotton samples shows that obvious bacteriostasis loops are observed around the samples with the appearance of both Gram-negative bacterium Escherichia coli (E. coli) and Gram-positive bacterium Bacillus subtilis (B. subtilis). The average diameter of the bacteriostasis loops against both E. coli and B. subtilis becomes larger with an increasing silver loading amount.This work provides a universal, fast, simple, and environmentally-friendly cold plasma method for synthesizing Ag NPs on heat-sensitive materials at atmospheric pressure.

Effect of Voltage and Oxygen on Inactivation of E. coli and S. typhi using Pulsed Dielectric Barrier Discharge

2021 ◽  
pp. 107879
Author(s):  
B.G. Rodríguez-Méndez ◽  
A.N. Hernández-Arias ◽  
D.G. Gutiérrez-León ◽  
R. López-Callejas ◽  
A. Mercado-Cabrera ◽  
...  
Keyword(s):  
Dielectric Barrier Discharge ◽  
Barrier Discharge ◽  
Dielectric Barrier ◽  
E Coli

Inactivation of E. coli and B. subtilis by a parallel-plate dielectric barrier discharge jet

2010 ◽  
Vol 204 (21-22) ◽  
pp. 3729-3737 ◽  
Author(s):  
M.H. Chiang ◽  
J.Y. Wu ◽  
Y.H. Li ◽  
J.S. Wu ◽  
S.H. Chen ◽  
...  
Keyword(s):  
Dielectric Barrier Discharge ◽  
Parallel Plate ◽  
Barrier Discharge ◽  
Dielectric Barrier ◽  
E Coli

Gas Flow Effect on E. coli and B. subtilis Bacteria Inactivation in Water Using a Pulsed Dielectric Barrier Discharge

2013 ◽  
Vol 41 (1) ◽  
pp. 147-154 ◽  
Author(s):  
Benjamín Gonzalo Rodriguez-Mendez ◽  
Alma Neli Hernandez-Arias ◽  
Régulo Lopez-Callejas ◽  
Raúl Valencia-Alvarado ◽  
Antonio Mercado-Cabrera ◽  
...  
Keyword(s):  
Dielectric Barrier Discharge ◽  
Gas Flow ◽  
Barrier Discharge ◽  
Dielectric Barrier ◽  
Flow Effect ◽  
E Coli ◽  
Bacteria Inactivation

scholarly journals The Efficiency of Atmospheric Dielectric Barrier Discharge Plasma against Escherichia coli and Bacillus cereus on Dried Laver (Porphyra tenera)

Foods ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 1013 ◽  
Author(s):  
Ji Yoon Kim ◽  
Eun Bi Jeon ◽  
Man-Seok Choi ◽  
Eun Ha Choi ◽  
Jun Sup Lim ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Bacillus Cereus ◽  
Dielectric Barrier Discharge ◽  
Barrier Discharge ◽  
Dbd Plasma ◽  
Dielectric Barrier ◽  
E Coli ◽  
Porphyra Tenera ◽  
Dried Laver ◽  
Atmospheric Dielectric Barrier Discharge

This study investigated the effects of atmospheric dielectric barrier discharge (DBD) plasma (1.1 kV, 43 kHz, 5–30 min, N2: 1.5 L/m) on the reduction of Escherichia coli and Bacillus cereus on dried laver. The reductions of E. coli and B. cereus by 5, 10, 20, and 30 min of DBD plasma were 0.56 and 0.24, 0.61 and 0.66, 0.76 and 1.24, and 1.02 and 1.38 log CFU/g, respectively. The D-value of E. coli and B. cereus was predicted as 29.80 and 20.53 min, respectively, using the Weibull model for E. coli (R2 = 0.95) and first-order kinetics for B. cereus (R2 = 0.94). After DBD plasma 5–30 min treatment, there was no change in pH (6.20–6.21) and this value was higher than the untreated dried laver (6.08). All sensory scores in DBD plasma-treated laver were determined as >6 points. The 30 min of DBD plasma is regarded as a novel intervention for the control of potential hazardous bacteria in dried laver.

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