Selective removal of cationic dyes using response surface methodology optimized gum acacia-sodium alginate blended superadsorbent

2019 ◽  
Vol 124 ◽  
pp. 331-345 ◽  
Author(s):  
Amit Kumar Sharma ◽  
Priya ◽  
Balbir Singh Kaith ◽  
Nisha Sharma ◽  
Jaspreet K. Bhatia ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Sodium Alginate ◽  
Selective Removal ◽  
Cationic Dyes ◽  
Gum Acacia

Optimization of Immobilization Conditions for Protease Extracted from Torpedo Scad (Megalaspis cordyla) Viscera Using Response Surface Methodology (RSM)

2019 ◽  
Vol 13 (2) ◽  
pp. 8
Author(s):  
Ali Jawad ◽  
Normah Ismail
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Sodium Alginate ◽  
Calcium Chloride ◽  
Proteolytic Activity ◽  
Crude Extract ◽  
Chloride Solution ◽  
Calcium Chloride Solution ◽  
Optimum Conditions ◽  
Immobilized Protease

Protease was extracted from the viscera of torpedo scad fish (Megalspis cordyla) to obtain the crude extract which was then partially purified in 70% ammonium sulphate. The collected precipitate was dialysed and subsequently immobilized in sodium alginate and calcium chloride solution. The optimum concentrations of sodium alginate and calcium chloride to produce the highest yield of immobilized protease was determined by using Response Surface Methodology (RSM). From the results, the optimum conditions obtained were 2.5% of sodium alginate and 0.25 M of calcium chloride achieving a yield of 55.52%. Thus, the utilization of 2.5% sodium alginate and 0.25 M calcium chloride as the immobilization media were able to produce yield of immobilized protease from torpedo scad viscera with the highest proteolytic activity.

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