PtrLAC16 plays a key role in catalyzing lignin polymerization in the xylem cell wall of Populus

2021 ◽  
Author(s):  
Yadi Liu ◽  
Shan Cao ◽  
Xiatong Liu ◽  
Ying Li ◽  
Bing Wang ◽  
...  
Keyword(s):  
Cell Wall ◽  
Xylem Cell ◽  
Lignin Polymerization

A cell wall-bound anionic peroxidase, PtrPO21, is involved in lignin polymerization in Populus trichocarpa

2016 ◽  
Vol 12 (2) ◽  
Author(s):  
Chien-Yuan Lin ◽  
Quanzi Li ◽  
Sermsawat Tunlaya-Anukit ◽  
Rui Shi ◽  
Ying-Hsuan Sun ◽  
...  
Keyword(s):  
Cell Wall ◽  
Populus Trichocarpa ◽  
Anionic Peroxidase ◽  
A Cell ◽  
Lignin Polymerization

scholarly journals A Novel Plasma Membrane-Anchored Protein Regulates Xylem Cell-Wall Deposition through Microtubule-Dependent Lateral Inhibition of Rho GTPase Domains

2017 ◽  
Vol 27 (16) ◽  
pp. 2522-2528.e4 ◽  
Author(s):  
Yuki Sugiyama ◽  
Mayumi Wakazaki ◽  
Kiminori Toyooka ◽  
Hiroo Fukuda ◽  
Yoshihisa Oda
Keyword(s):  
Cell Wall ◽  
Plasma Membrane ◽  
Lateral Inhibition ◽  
Rho Gtpase ◽  
Xylem Cell ◽  
Wall Deposition ◽  
Cell Wall Deposition

Cadmium - citric acid - xylem cell wall interactions in tomato plants

1992 ◽  
Vol 15 (1) ◽  
pp. 71-79 ◽  
Author(s):  
M. H. M. N. SENDEN ◽  
F. J. M. PAASSEN ◽  
A. J. G. M. MEER ◽  
H. Th. WOLTERBEEK
Keyword(s):  
Cell Wall ◽  
Citric Acid ◽  
Tomato Plants ◽  
Xylem Cell ◽  
Wall Interactions

scholarly journals Identifying transcription factors that reduce wood recalcitrance and improve enzymatic degradation of xylem cell wall in Populus

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Chiaki Hori ◽  
Naoki Takata ◽  
Pui Ying Lam ◽  
Yuki Tobimatsu ◽  
Soichiro Nagano ◽  
...  
Keyword(s):  
Cell Wall ◽  
Populus Tremuloides ◽  
Cell Walls ◽  
Enzymatic Degradation ◽  
Plant Biomass ◽  
Lignin Content ◽  
Enzymatic Saccharification ◽  
Wall Structure ◽  
Xylem Cell

AbstractDeveloping an efficient deconstruction step of woody biomass for biorefinery has been drawing considerable attention since its xylem cell walls display highly recalcitrance nature. Here, we explored transcriptional factors (TFs) that reduce wood recalcitrance and improve saccharification efficiency in Populus species. First, 33 TF genes up-regulated during poplar wood formation were selected as potential regulators of xylem cell wall structure. The transgenic hybrid aspens (Populus tremula × Populus tremuloides) overexpressing each selected TF gene were screened for in vitro enzymatic saccharification. Of these, four transgenic seedlings overexpressing previously uncharacterized TF genes increased total glucan hydrolysis on average compared to control. The best performing lines overexpressing Pt × tERF123 and Pt × tZHD14 were further grown to form mature xylem in the greenhouse. Notably, the xylem cell walls exhibited significantly increased total xylan hydrolysis as well as initial hydrolysis rates of glucan. The increased saccharification of Pt × tERF123-overexpressing lines could reflect the improved balance of cell wall components, i.e., high cellulose and low xylan and lignin content, which could be caused by upregulation of cellulose synthase genes upon the expression of Pt × tERF123. Overall, we successfully identified Pt × tERF123 and Pt × tZHD14 as effective targets for reducing cell wall recalcitrance and improving the enzymatic degradation of woody plant biomass.

scholarly journals Synchrotron FTIR and Raman spectroscopy 1 ectroscopy provide unique spectral fingerprints for Arabidopsis floral stem vascular tissues

2018 ◽  
Author(s):  
S Dinant ◽  
N Wolff ◽  
F De Marco ◽  
F Vilaine ◽  
L Gissot ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Cell Wall ◽  
Cell Walls ◽  
Double Mutant ◽  
Vascular Tissue ◽  
Cell Wall Composition ◽  
Specific Cell ◽  
Xylem Cell ◽  
Phloem Cell ◽  
Ftir And Raman Spectroscopy

AbstractCell walls are highly complex structures that are modified during plant growth and development. For example, the development of phloem and xylem vascular cells, which participate in the transport of sugars and water as well as support, can be influenced by cell-specific cell wall composition. Here, we used synchrotron radiation-based infrared (SR-FTIR) and Raman spectroscopy to analyze the cell wall composition of wild-type and double mutant sweet11-1sweet12-1, which impairs sugar transport, Arabidopsis floral stem vascular tissue. The SR-FTIR spectra showed that in addition to modified xylem cell wall composition, phloem cell walls in the double mutant line were characterized by modified hemicellulose composition. Moreover, combining Raman spectroscopy with a Classification and Regression Tree (CART) method identified combinations of Raman shifts that could distinguish xylem vessels and fibers. Additionally, the disruption of SWEET11 and SWEET12 genes impacts xylem cell wall composition in a cell-specific manner, with changes in hemicelluloses and cellulose observed at the xylem vessel interface. These results suggest that the facilitated transport of sugars by transporters that exist between vascular parenchyma cells and conducting cells is important to ensuring correct phloem and xylem cell wall composition.HighlightCombining vibrational spectroscopy techniques and multivariate analysis shows that the disruption of SWEET genes impacts phloem cell wall composition and that the effect on xylem cell wall composition is cell-specific.

scholarly journals An AP 2/ ERF transcription factor ERF 139 coordinates xylem cell expansion and secondary cell wall deposition

2019 ◽  
Vol 224 (4) ◽  
pp. 1585-1599 ◽  
Author(s):  
Bernard Wessels ◽  
Carolin Seyfferth ◽  
Sacha Escamez ◽  
Thomas Vain ◽  
Kamil Antos ◽  
...  
Keyword(s):  
Cell Wall ◽  
Transcription Factor ◽  
Cell Expansion ◽  
Secondary Cell Wall ◽  
Xylem Cell ◽  
Wall Deposition ◽  
Cell Wall Deposition ◽  
Erf Transcription Factor

scholarly journals Populus PtERF85 Balances Xylem Cell Expansion and Secondary Cell Wall Formation in Hybrid Aspen

Cells ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1971
Author(s):  
Carolin Seyfferth ◽  
Bernard A. Wessels ◽  
Jorma Vahala ◽  
Jaakko Kangasjärvi ◽  
Nicolas Delhomme ◽  
...  
Keyword(s):  
Cell Wall ◽  
Cell Expansion ◽  
Secondary Cell Wall ◽  
Secondary Growth ◽  
Ectopic Expression ◽  
Transcriptional Networks ◽  
Ethylene Response Factor ◽  
Transgenic Trees ◽  
Hybrid Aspen ◽  
Xylem Cell

Secondary growth relies on precise and specialized transcriptional networks that determine cell division, differentiation, and maturation of xylem cells. We identified a novel role for the ethylene-induced Populus ethylene response factor PtERF85 (Potri.015G023200) in balancing xylem cell expansion and secondary cell wall (SCW) formation in hybrid aspen (Populus tremula x tremuloides). Expression of PtERF85 is high in phloem and cambium cells and during the expansion of xylem cells, while it is low in maturing xylem tissue. Extending PtERF85 expression into SCW forming zones of woody tissues through ectopic expression reduced wood density and SCW thickness of xylem fibers but increased fiber diameter. Xylem transcriptomes from the transgenic trees revealed transcriptional induction of genes involved in cell expansion, translation, and growth. The expression of genes associated with plant vascular development and the biosynthesis of SCW chemical components such as xylan and lignin, was down-regulated in the transgenic trees. Our results suggest that PtERF85 activates genes related to xylem cell expansion, while preventing transcriptional activation of genes related to SCW formation. The importance of precise spatial expression of PtERF85 during wood development together with the observed phenotypes in response to ectopic PtERF85 expression suggests that PtERF85 contributes to the transition of fiber cells from elongation to secondary cell wall deposition.

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