scholarly journals Genetic and biochemical characterization of an oligo-α-1,6-glucosidase from Lactobacillus plantarum

2017 ◽  
Vol 246 ◽  
pp. 32-39 ◽  
Author(s):  
Susana Delgado ◽  
Ana Belén Flórez ◽  
Lucía Guadamuro ◽  
Baltasar Mayo
Keyword(s):  
Lactobacillus Plantarum ◽  
Biochemical Characterization

Three Systems for Biochemical Characterization of Lactobacilli Associated with Meat Spoilage

1984 ◽  
Vol 47 (12) ◽  
pp. 937-942 ◽  
Author(s):  
B. H. LEE ◽  
R. E. SIMARD
Keyword(s):  
Lactobacillus Plantarum ◽  
Biochemical Characterization ◽  
Generic Level ◽  
Meat Spoilage

Three methods (APIZYM, Minitek and VPI systems) were compared for their ability to characterize some of lactobacillus isolates from anaerobically spoiled meats. Nineteen authentic lactobacillus cultures obtained from various sources were also compared. Greening spoilage isolates from frankfurters were identified as Lactobacillus viridescens by the APIZYM and Minitek tests. Lactobacillus isolates from spoiled veal were identified as Lactobacillus plantarum by one of the three methods. Other isolates which could not be assigned to a generic level appeared to be atypical streptobacteria or betabacteria. Except for some disagreement in the identity of lactobacilli among the three systems, they proved useful in distinguishing between the different lactobacilli.

scholarly journals Phenotypic and molecular characterization of different isolates of Lactobacillus plantarum from four Nigerian fermented foods for use as probiotics in aquaculture

2018 ◽  
Vol 5 (10) ◽  
pp. 329-337
Author(s):  
Daniel Olusegun Diyaolu ◽  
Fatuyi Olanipekun Ekundayo ◽  
Emmanuel Adedayo Fasakin ◽  
Olabode Thomas Adebayo
Keyword(s):  
16S Rrna ◽  
Lactobacillus Plantarum ◽  
Biochemical Characterization ◽  
Bacterial Species ◽  
Fermented Food ◽  
Rrna Gene ◽  
Fermented Foods ◽  
Slight Variation ◽  
16S Rna

This study aimed at identifying Lactobacillus plantarum from fermented maize, sorghum, soyabeans and cassava, using both phenotypic method and 16S RNA sequencing, as well as determining similarity or otherwise among recovered isolates. Biochemical characterization of isolates recovered from these fermented foods revealed that L. plantarum occurred in all fermented food examined, with slight variation in their abilities to ferment some sugars (arabinose, dulbitol and mannitol). These phenotypically identified isolates were also confirmed to be L. plantarum by 16S rRNA sequencing, having close relatedness (> 95%) with other isolates available in the gene bank. However, intragenomic heterogeneity of the 16S rRNA gene was observed among these L. plantarum isolates. The result obtained in this finding pinpoints the need to evaluate the beneficial effects each strain of L. plantarum may possess as promising probiotics, rather than generalising common effects for all strains of this bacterial species.

scholarly journals Physiological and biochemical characterization of the two α-l-rhamnosidases of Lactobacillus plantarum NCC245

Microbiology ◽  
2009 ◽  
Vol 155 (8) ◽  
pp. 2739-2749 ◽  
Author(s):  
Marta Ávila ◽  
Muriel Jaquet ◽  
Deborah Moine ◽  
Teresa Requena ◽  
Carmen Peláez ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Lactobacillus Plantarum ◽  
Carbon Catabolite Repression ◽  
Biochemical Characterization ◽  
Residual Activity ◽  
Maximal Activity ◽  
Growth Conditions ◽  
Transcriptional Level ◽  
Physiological And Biochemical

This work is believed to be the first report on the physiological and biochemical characterization of α-l-rhamnosidases in lactic acid bacteria. A total of 216 strains representing 37 species and eight genera of food-grade bacteria were screened for α-l-rhamnosidase activity. The majority of positive bacteria (25 out of 35) were Lactobacillus plantarum strains, and activity of the L. plantarum strain NCC245 was examined in more detail. The analysis of α-l-rhamnosidase activity under different growth conditions revealed dual regulation of the enzyme activity, involving carbon catabolite repression and induction: the enzyme activity was downregulated by glucose and upregulated by l-rhamnose. The expression of the two α-l-rhamnosidase genes rhaB1 and rhaB2 and two predicted permease genes rhaP1 and rhaP2, identified in a probable operon rhaP2B2P1B1, was repressed by glucose and induced by l-rhamnose, showing regulation at the transcriptional level. The two α-l-rhamnosidase genes were overexpressed and purified from Escherichia coli. RhaB1 activity was maximal at 50 °C and at neutral pH and RhaB2 maximal activity was detected at 60 °C and at pH 5, with high residual activity at 70 °C. Both enzymes showed a preference for the α-1,6 linkage of l-rhamnose to β-d-glucose, hesperidin and rutin being their best substrates, but, surprisingly, no activity was detected towards the α-1,2 linkage in naringin under the tested conditions. In conclusion, we identified and characterized the strain L. plantarum NCC245 and its two α-l-rhamnosidase enzymes, which might be applied for improvement of bioavailability of health-beneficial polyphenols, such as hesperidin, in humans.

scholarly journals Molecular Characterisation of Bacterial Isolates from Vegetable, Cow Milk and Locust Bean Samples

2020 ◽  
pp. 139-147
Author(s):  
T. A. Ihum ◽  
O. O. Efunwole ◽  
S. O. Olarewaju ◽  
E. B. Akinro ◽  
O. R. Adebayo ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Biochemical Characterization ◽  
Molecular Level ◽  
Physiological State ◽  
Food Samples ◽  
Cow Milk ◽  
Carotovorum Subsp ◽  
Biochemical State ◽  
Locust Bean

Molecular characterization involves characterization at molecular level without any effect of environment or development or physiological state of the organism. Biochemical characterization is the characterization of the biochemical state of the organism, which is in fact affected by environment, development as well as physiological state. The objective of this study was to molecularly characterize bacteria isolated from certain food samples. Five bacteria isolates were obtained from the vegetable samples while two LAB isolates were obtained from cow milk and locust bean samples. The bacteria isolates were identified using 16SRNA GENE sequencing using the BLAST algorithm and were identified as Staphylococcus aureus CIP 9973; Pectobacterium carotovorum subsp. carotovorum Pec 1; Enterobacter cloacae AS10 Klebsiella aerogenes OFM28; Escherichia coli 2013C-3342; Proteus mirabilis UPMSD3; Lactobacillus plantarum NCU116; Lactobacillus plantarum NRIC 0383. Characterization of bacteria isolates to molecular level is of enormous advantage as it helps to know the exact genus of a particular organism.

Overexpression, Purification, and Biochemical Characterization of the Esterase Est0796 from Lactobacillus plantarum WCFS1

2012 ◽  
Vol 54 (2) ◽  
pp. 651-660 ◽  
Author(s):  
Navarro-González Inmaculada ◽  
Sánchez-Ferrer Álvaro ◽  
García-Carmona Francisco
Keyword(s):  
Lactobacillus Plantarum ◽  
Biochemical Characterization

scholarly journals Erratum to: Overexpression, Purification, and Biochemical Characterization of the Esterase Est0796 from Lactobacillus plantarum WCFS1

2013 ◽  
Vol 55 (2) ◽  
pp. 168-168
Author(s):  
Inmaculada Navarro-González ◽  
Álvaro Sánchez-Ferrer ◽  
Francisco García-Carmona
Keyword(s):  
Lactobacillus Plantarum ◽  
Biochemical Characterization

Plant Pathology Diagnosed by X-Ray Microanalysis

1987 ◽  
Vol 45 ◽  
pp. 974-975
Author(s):  
J. H. Resau ◽  
N. Howell ◽  
S. H. Chang
Keyword(s):  
Electron Microscopy ◽  
Plant Pathology ◽  
Biochemical Characterization ◽  
Nutrient Deficiency ◽  
Green Leaf ◽  
Parasitic Infestation ◽  
X Ray

Spinach grown in Texas developed “yellow spotting” on the peripheral portions of the leaves. The exact cause of the discoloration could not be determined as there was no evidence of viral or parasitic infestation of the plants and biochemical characterization of the plants did not indicate any significant differences between the yellow and green leaf portions of the spinach. The present study was undertaken using electron microscopy (EM) to determine if a micro-nutrient deficiency was the cause for the discoloration.Green leaf spinach was collected from the field and sent by express mail to the EM laboratory. The yellow and equivalent green portions of the leaves were isolated and dried in a Denton evaporator at 10-5 Torr for 24 hrs. The leaf specimens were then examined using a JEOL 100 CX analytical microscope. TEM specimens were prepared according to the methods of Trump et al.

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