Molecular typing of clinical isolates of Stenotrophomonas maltophilia by pulsed-field gel electrophoresis and random primer PCR fingerprinting

2008 ◽  
Vol 211 (3-4) ◽  
pp. 292-298 ◽  
Author(s):  
Reiner Schaumann ◽  
Franziska Laurin ◽  
Arne C. Rodloff
Keyword(s):  
Molecular Typing ◽  
Gel Electrophoresis ◽  
Stenotrophomonas Maltophilia ◽  
Pulsed Field Gel Electrophoresis ◽  
Clinical Isolates ◽  
Random Primer ◽  
Pulsed Field ◽  
Pcr Fingerprinting

scholarly journals Smqnr VARIANTS IN CLINICAL ISOLATES OF Stenotrophomonas maltophilia IN BRAZIL

2013 ◽  
Vol 55 (6) ◽  
pp. 417-420 ◽  
Author(s):  
Jorge Isaac Gracia-Paez ◽  
Juliana Rosa Ferraz ◽  
Ivan Avelino Franca E Silva ◽  
Flavia Rossi ◽  
Anna Sara Levin ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Stenotrophomonas Maltophilia ◽  
Pulsed Field Gel Electrophoresis ◽  
Pcr Detection ◽  
Clinical Isolates ◽  
Intrinsic Resistance ◽  
Pulsed Field ◽  
First Report ◽  
Microdilution Method ◽  
Novel Variants

SUMMARY Stenotrophomonas maltophilia contains a novel chromosomally-encoded qnr gene named Smqnr that contributes to low intrinsic resistance to quinolone. We described Smqnr in 13 clinical isolates of S. maltophilia from two Brazilian hospitals, over a 2-year period. The strains were identified by API 20 NE (bioMérieux, France). Susceptibility by microdilution method to trimetroprim/sulfamethoxazole, ciprofloxacin, levofloxacin, minocycline, ceftazidime, chloramphenicol and ticarcillin/clavulanate was performed according to CLSI. PCR detection of Smqnr gene was carried out. The sequence of Smqnr was compared with those deposited in GenBank. Pulsed-field gel electrophoresis (PFGE) of all strains was performed. Thirteen Smqnr positives isolates were sequenced and three novel variants of Smqnr were identified. All 13 Smqnr isolates had distinguishable patterns by PFGE. This is the first report of Smqnr in S. maltophilia isolated in Brazil.

scholarly journals Molecular typing of Escherichia coli O157[ratio ]H7 (H−) isolates from cattle in Japan

1999 ◽  
Vol 122 (2) ◽  
pp. 337-341 ◽  
Author(s):  
M. AKIBA ◽  
T. MASUDA ◽  
T. SAMESHIMA ◽  
K. KATSUDA ◽  
M. NAKAZAWA
Keyword(s):  
Escherichia Coli ◽  
Molecular Typing ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Escherichia Coli O157 ◽  
Outbreak Strain ◽  
Pulsed Field ◽  
E Coli ◽  
Biological Methods ◽  
Pfge Profiles

A total of 77 Escherichia coli O157[ratio ]H7 (H−) isolates from cattle in Japan were investigated by molecular biological methods. Most of these isolates (43 isolates) possessed the stx2 gene, but not stx1. Fifteen bacteriophage types and 50 pulsed-field gel electrophoresis (PFGE) profiles were observed. One isolate was indistinguishable from the human outbreak strain by these methods. This indicates that cattle must be considered as a possible source of human E. coli O157[ratio ]H7 infection in Japan.

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