Molecular typing of Staphylococcus aureus clinical isolates by pulsed-field gel electrophoresis, staphylococcal cassette chromosome mec type determination and dissemination of antibiotic resistance genes

2007 ◽  
Vol 30 (6) ◽  
pp. 505-513 ◽  
Author(s):  
Leticia Millán Laplana ◽  
M Pilar Goñi Cepero ◽  
Joaquim Ruiz ◽  
Paula Cerdá Zolezzi ◽  
M Carmen Rubio Calvo ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Molecular Typing ◽  
Gel Electrophoresis ◽  
Antibiotic Resistance Genes ◽  
Pulsed Field Gel Electrophoresis ◽  
Clinical Isolates ◽  
Pulsed Field ◽  
Staphylococcal Cassette Chromosome

scholarly journals Multiple Clones within Multidrug-ResistantSalmonella enterica Serotype Typhimurium Phage Type DT104

2000 ◽  
Vol 38 (3) ◽  
pp. 1269-1271 ◽  
Author(s):  
Antonis Markogiannakis ◽  
Panayotis T. Tassios ◽  
Maria Lambiri ◽  
Linda R. Ward ◽  
Jenny Kourea-Kremastinou ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Gel Electrophoresis ◽  
Salmonella Enterica ◽  
Phage Type ◽  
Antibiotic Resistance Genes ◽  
Multidrug Resistant ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field

Six distinct clones were present among Greek multidrug-resistantSalmonella enterica serotype Typhimurium phage type DT104, since isolates belonging to resistance phenotypes including the ACSSuT (ampicillin, chloramphenicol, streptomycin, sulfonamides, and tetracycline) core could be distinguished with respect to their pulsed-field gel electrophoresis patterns, int1 integron structures, and presence or absence of antibiotic resistance genesant(3")-Ia, pse-1, and tem-1.

scholarly journals Characterization of antimicrobial resistance genes inHaemophilus parasuisisolated from pigs in China

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4613 ◽  
Author(s):  
Yongda Zhao ◽  
Lili Guo ◽  
Jie Li ◽  
Xianhui Huang ◽  
Binghu Fang
Keyword(s):  
Antibiotic Resistance ◽  
Antimicrobial Resistance ◽  
Resistance Genes ◽  
Gel Electrophoresis ◽  
Antibiotic Resistance Genes ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Antimicrobial Resistance Genes ◽  
Broth Microdilution Method

BackgroundHaemophilus parasuisis a common porcine respiratory pathogen that causes high rates of morbidity and mortality in farmed swine. We performed a molecular characterization of antimicrobial resistance genes harbored byH. parasuisfrom pig farms in China.MethodsWe screened 143H. parasuisisolates for antimicrobial susceptibility against six fluoroquinolone antibiotics testing by the broth microdilution method, and the presence of 64 antimicrobial resistance genes by PCR amplification and DNA sequence analysis. We determined quinolone resistance determining region mutations of DNA gyrase (gyrAandgyrB) and topoisomerase IV (parCandparE). The genetic relatedness among the strains was analyzed by pulsed-field gel electrophoresis.ResultsSusceptibility test showed that all isolates were low resistance to lomefloxacin (28.67%), levofloxacin (20.28%), norfloxacin (22.38%), ciprofloxacin (23.78%), however, high resistance levels were found to nalidixic acid (82.52%) and enrofloxacin (55.94%). In addition, we found 14 antimicrobial resistance genes were present in these isolates, includingblaTEM-1, blaROB-1,ermB, ermA, flor, catl, tetB, tetC, rmtB, rmtD, aadA1, aac(3′)-llc, sul1, and sul2genes. Interestingly, one isolate carried five antibiotic resistance genes (tetB, tetC, flor, rmtB, sul1). The genestetB,rmtB,andflorwere the most prevalent resistance genes inH. parasuisin China. Alterations in thegyrAgene (S83F/Y, D87Y/N/H/G) were detected in 81% of the strains andparCmutations were often accompanied by agyrAmutation. Pulsed-field gel electrophoresis typing revealed 51 unique patterns in the isolates carrying high-level antibiotic resistance genes, indicating considerable genetic diversity and suggesting that the genes were spread horizontally.DiscussionThe current study demonstrated that the high antibiotic resistance ofH. parasuisin piglets is a combination of transferable antibiotic resistance genes and multiple target gene mutations. These data provide novel insights for the better understanding of the prevalence and epidemiology of antimicrobial resistance inH. parasuis.

scholarly journals Characterization of antimicrobial resistance genes in Haemophilus parasuis isolated from pigs in China

2017 ◽  
Author(s):  
Yongda Zhao ◽  
Lili Guo ◽  
Jie Li ◽  
Xianhui Huang ◽  
Binghu Fang
Keyword(s):  
Antibiotic Resistance ◽  
Antimicrobial Resistance ◽  
Resistance Genes ◽  
Gel Electrophoresis ◽  
Antibiotic Resistance Genes ◽  
Pulsed Field Gel Electrophoresis ◽  
Haemophilus Parasuis ◽  
Pulsed Field ◽  
Antimicrobial Resistance Genes

Background: Haemophilus parasuis is a common porcine respiratory disease that causes high rates of morbidity and mortality in farmed swine. We performed a molecular characterization of antimicrobial resistance genes harbored by H. parasuis from pig farms in China. Methods: We screened 143 H. parasuis isolates for the presence of 64 antimicrobial resistance genes by PCR amplification and DNA sequence analysis. We determined quinolone resistance determining region mutations of DNA gyrase (gyrA and gyrB) and topoisomerase IV (parC and parE). The genetic relatedness among the strains was analyzed by pulsed-field gel electrophoresis. Results: We found 14 antimicrobial resistance genes were present in these isolates, including TEM-1, ROB-1.ermB,ermA ,flor, catl,tetB,tetC, rmtB, rmtD, aadA1, aac(3’)-ⅡC, sul1, and sul2 genes. Interestingly, one isolate carried 5 antibiotic resistance genes (tetB, tetC, flor, rmtB, sul1). The genes tetB, rmtB, and flor were the most prevalent resistance genes in H. parasuis in China. Alterations in the gyrA gene (S83F/Y, D87Y/N/H/G) were detected in 81% of the strains and parC mutations were often accompanied by a gyrA mutation. pulsed-field gel electrophoresis typing revealed 51 unique patterns in the isolates carrying antibiotic resistance genes indicating considerable genetic diversity and suggesting the genes were spread horizontally. Discussion: The current study demonstrated that the high antibiotic resistance of H. parasuis in piglets is a combination of transferable antibiotic resistance genes and multiple target gene mutations. GyrA gene mutation also was the most important role in quinolone resistance. These data provide novel insights for the better understanding of the prevalence and epidemiology of antimicrobial resistance in H. parasuis.

scholarly journals Characterization of antimicrobial resistance genes in Haemophilus parasuis isolated from pigs in China

2017 ◽  
Author(s):  
Yongda Zhao ◽  
Lili Guo ◽  
Jie Li ◽  
Xianhui Huang ◽  
Binghu Fang
Keyword(s):  
Antibiotic Resistance ◽  
Antimicrobial Resistance ◽  
Resistance Genes ◽  
Gel Electrophoresis ◽  
Antibiotic Resistance Genes ◽  
Pulsed Field Gel Electrophoresis ◽  
Haemophilus Parasuis ◽  
Pulsed Field ◽  
Antimicrobial Resistance Genes

Background: Haemophilus parasuis is a common porcine respiratory disease that causes high rates of morbidity and mortality in farmed swine. We performed a molecular characterization of antimicrobial resistance genes harbored by H. parasuis from pig farms in China. Methods: We screened 143 H. parasuis isolates for the presence of 64 antimicrobial resistance genes by PCR amplification and DNA sequence analysis. We determined quinolone resistance determining region mutations of DNA gyrase (gyrA and gyrB) and topoisomerase IV (parC and parE). The genetic relatedness among the strains was analyzed by pulsed-field gel electrophoresis. Results: We found 14 antimicrobial resistance genes were present in these isolates, including TEM-1, ROB-1.ermB,ermA ,flor, catl,tetB,tetC, rmtB, rmtD, aadA1, aac(3’)-ⅡC, sul1, and sul2 genes. Interestingly, one isolate carried 5 antibiotic resistance genes (tetB, tetC, flor, rmtB, sul1). The genes tetB, rmtB, and flor were the most prevalent resistance genes in H. parasuis in China. Alterations in the gyrA gene (S83F/Y, D87Y/N/H/G) were detected in 81% of the strains and parC mutations were often accompanied by a gyrA mutation. pulsed-field gel electrophoresis typing revealed 51 unique patterns in the isolates carrying antibiotic resistance genes indicating considerable genetic diversity and suggesting the genes were spread horizontally. Discussion: The current study demonstrated that the high antibiotic resistance of H. parasuis in piglets is a combination of transferable antibiotic resistance genes and multiple target gene mutations. GyrA gene mutation also was the most important role in quinolone resistance. These data provide novel insights for the better understanding of the prevalence and epidemiology of antimicrobial resistance in H. parasuis.

scholarly journals Comparison of Protein A Gene Sequencing with Pulsed-Field Gel Electrophoresis and Epidemiologic Data for Molecular Typing of Methicillin-Resistant Staphylococcus aureus

2000 ◽  
Vol 38 (4) ◽  
pp. 1347-1351 ◽  
Author(s):  
Yi-Wei Tang ◽  
Michael G. Waddington ◽  
Douglas H. Smith ◽  
Janice M. Manahan ◽  
Peggy C. Kohner ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Sequence Analysis ◽  
Molecular Typing ◽  
Gel Electrophoresis ◽  
Protein A ◽  
Pulsed Field Gel Electrophoresis ◽  
Sequencing Analysis ◽  
Methicillin Resistant ◽  
Pulsed Field ◽  
Epidemic Period

The epidemiologic relatedness of methicillin-resistantStaphylococcus aureus (MRSA) isolates is currently determined by analysis of chromosomal DNA restriction patterns by pulsed-field gel electrophoresis (PFGE). We have evaluated an alternative typing system (MicroSeq StaphTrack Kit; Perkin-Elmer Biosystems) based on the sequence analysis of the chromosomally encoded polymorphic repeat X region of the S. aureus protein A (spa) gene. A total of 69 clinical MRSA isolates were divided into 18 groups according to the number and nucleotide sequences of the spa repeats. Molecular typing results obtained both by spa sequencing and from the PFGE patterns were concordant except for one group, which contained 20 isolates recovered over a 2-year period from hospitalized patients at the Mayo Clinic. Although the spa typing patterns were indistinguishable for those isolates, PFGE analysis yielded seven related but distinguishable patterns. Further coagulase gene sequence analysis subtyped those 20 strains into four groups which followed distinct temporal and geographic distributions. During a 2-year epidemic period there were up to 7 fragment changes in PFGE patterns among epidemiologically related isolates, suggesting that PFGE may be unsuitable for long-term typing of strains involved in epidemics. Although more limited than PFGE in discriminatory power, spa sequencing analysis could be used as a screening method for typing of MRSA strains because of the shorter turnaround time, ease of use, and the inherent advantages of sequence analysis, storage, and sharing of information.

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