scholarly journals Acquired 16s methyl transferase associated high level aminoglycoside resistance in Acinetobacter baumannii recovered from ICU patients from a tertiary referral hospital of northeast India

2016 ◽  
Vol 45 ◽  
pp. 46
Author(s):  
S. Upadhyay ◽  
S.R. Joshi ◽  
A.B. Khryiem ◽  
P. Bhattacharyya
Keyword(s):  
Acinetobacter Baumannii ◽  
Northeast India ◽  
Referral Hospital ◽  
Tertiary Referral ◽  
Tertiary Referral Hospital ◽  
Aminoglycoside Resistance ◽  
Icu Patients ◽  
Methyl Transferase ◽  
High Level

scholarly journals Co-infection of ST2IP carbapenem-resistant Acinetobacter baumannii with SARS-CoV-2 in the patients admitted to a Tehran tertiary referral hospital

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Alireza Abdollahi ◽  
Amir Aliramezani ◽  
Mohammadreza Salehi ◽  
Mahsa Norouzi Shadehi ◽  
Sedighe Ghourchian ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Genetic Relatedness ◽  
Carbapenem Resistance ◽  
Underlying Disease ◽  
Referral Hospital ◽  
Clinical Samples ◽  
Tertiary Referral ◽  
Tertiary Referral Hospital ◽  
Carbapenem Resistant ◽  
High Level

Abstract Background Carbapenem-resistant Acinetobacter baumannii (CRAB) is among the most concerning cause of healthcare-associated infections (HAI) due to its high level of antibiotic resistance and high mortality. In the era of the COVID-19 pandemic, the key priority of infection control committees is to contain the dissemination of antibiotic resistant Gram-negative bacteria. Here, we aimed to timely recognize the emergence of CRAB in COVID-19 cases admitted to the wards of a tertiary referral hospital and to identify the genetic relatedness of the isolates. Methods From 30 March to 30 May 2020, a total of 242 clinical samples from COVID-19 cases were screened for CRAB isolates using standard microbiologic and antibiotic susceptibility tests. The PCRs targeting oxa23, oxa24, oxa58, blaTEM and blaNDM-1 genes were performed. Two multiplex PCRs for identifying the global clones (GC) of A. baumannii were also performed. The sequence type of CRABs was determined using Institut Pasteur (IP) multilocus sequence typing (MLST) scheme. Results Eighteen CRAB isolates were recovered from COVID-19 patients with the mean age of 63.94 ± 13.8 years. All but 4 COVID-19 patients co-infected with CRAB were suffering from an underlying disease. Death was recorded as the outcome in ICUs for 9 (50%) COVID-19 patients co-infected with CRAB. The CRAB isolates belong to GC2 and ST2IP and carried the oxa23 carbapenem resistance gene. Conclusion This study demonstrated the co-infection of CRAB isolates and SARS-CoV-2 in the patients admitted to different ICUs at a referral hospital in Tehran. The CRAB isolates were found to belong to ST2IP, share the oxa23 gene and to have caused several outbreaks in the wards admitting COVID-19 patients.

Propagation of blaKPC-2 within two sequence types of Escherichia coli in a tertiary referral hospital of Northeast India.

Gene Reports ◽  
2021 ◽  
pp. 101283
Author(s):  
Bhaskar Jyoti Das ◽  
Jayalaxmi Wangkheimayum ◽  
K. Melson Singha ◽  
Deepshikha Bhowmik ◽  
Debadatta Dhar ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Northeast India ◽  
Referral Hospital ◽  
Tertiary Referral ◽  
Tertiary Referral Hospital ◽  
Sequence Types

scholarly journals Occurrence of Acquired 16S rRNA Methyltransferase-Mediated Aminoglycoside Resistance in Clinical Isolates of Enterobacteriaceae within a Tertiary Referral Hospital of Northeast India

2017 ◽  
Vol 61 (6) ◽  
Author(s):  
Jayalaxmi Wangkheimayum ◽  
Deepjyoti Paul ◽  
Debadatta Dhar ◽  
Rajlakshmi Nepram ◽  
Shiela Chetri ◽  
...  
Keyword(s):  
16S Rrna ◽  
Hospital Setting ◽  
Referral Hospital ◽  
Clinical Isolates ◽  
Tertiary Referral ◽  
Pcr Assay ◽  
Tertiary Referral Hospital ◽  
Aminoglycoside Resistance ◽  
Content Type ◽  
Resistance Determinants

ABSTRACT The methylation of a ribosomal target leads to a high level of resistance to all clinically relevant aminoglycoside antibiotics, so early detection of these resistance determinants will help to reduce the incidence of treatment failures as well as lessen the dissemination rate. Here, we characterized different 16S rRNA methyltransferases responsible for aminoglycoside resistance and their epidemiological background in clinical isolates of Enterobacteriaceae in a tertiary referral hospital in India. All aminoglycoside-resistant isolates were screened for different 16S rRNA methyltransferases by PCR assay, and incompatibility typing of the conjugable plasmid harboring resistance genes was performed by PCR-based replicon typing. An assay for the stability and elimination of these resistance plasmids was performed. The coexistence of extended-spectrum β-lactamases and metallo-β-lactamases was also detected, and the heterogeneity of these isolates was determined by enterobacterial repetitive intergenic consensus PCR. The PCR assay revealed the presence of armA, rmtA, rmtB, rmtC, and rmtD in single and multiple combinations, and these were carried by a diverse group of Inc plasmids. Plasmids harboring these resistance determinants were highly stable and maintained until the 55th serial passage, but SDS treatment could easily eliminate the plasmids harboring the resistance determinants. The coexistence of bla TEM, bla PER, bla GES, and bla SHV, as well as bla VIM and bla NDM, within these isolates was also detected. Strains with different clonal patterns of aminoglycoside resistance were found to spread in this hospital setting. We observed that the 16S rRNA methyltransferase genes were encoded within different Inc plasmid types, suggesting diverse origins and sources of acquisition. Therefore, the present study is of epidemiological importance and can have a role in infection control policy in hospital settings.

scholarly journals Carriage of blaNDM-1 in Pseudomonas aeruginosa through multiple Inc type plasmids in a tertiary referral hospital of northeast India

2016 ◽  
Vol 143 (6) ◽  
pp. 826 ◽  
Author(s):  
Amitabha Bhattacharjee ◽  
Deepjyoti Paul ◽  
AnandPrakash Maurya ◽  
DebadattaDhar Chanda ◽  
GauriDutt Sharma ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Northeast India ◽  
Referral Hospital ◽  
Tertiary Referral ◽  
Tertiary Referral Hospital

Micafungin use in a UK tertiary referral hospital

2018 ◽  
Vol 15 ◽  
pp. 82-87 ◽  
Author(s):  
David A. Enoch ◽  
Michael E. Murphy ◽  
Christianne Micallef ◽  
Huina Yang ◽  
Nicholas M. Brown ◽  
...  
Keyword(s):  
Referral Hospital ◽  
Tertiary Referral ◽  
Tertiary Referral Hospital
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