Molecular typing of Legionella pneumophila serogroup 1 clinical strains isolated in Italy

2014 ◽  
Vol 304 (5-6) ◽  
pp. 597-602 ◽  
Author(s):  
Stefano Fontana ◽  
Maria Scaturro ◽  
Maria Cristina Rota ◽  
Maria Grazia Caporali ◽  
Maria Luisa Ricci
Keyword(s):  
Legionella Pneumophila ◽  
Molecular Typing ◽  
Clinical Strains

Molecular typing of the Legionella pneumophila population isolated from several locations in a contaminated water network

2004 ◽  
Vol 50 (1) ◽  
pp. 281-285 ◽  
Author(s):  
A.-S. Lepeuple ◽  
M. Jovic ◽  
M.-R. de Roubin
Keyword(s):  
Legionella Pneumophila ◽  
Molecular Typing ◽  
Random Amplified Polymorphic Dna ◽  
Hot Water ◽  
Water Network ◽  
Rapd Pattern ◽  
Different Populations ◽  
Definition Of ◽  
Different Strains ◽  
Key Steps

The RAPD (random amplified polymorphic DNA) technique has been developed for the molecular typing of Legionella in order to characterise the populations of hot water systems. During this study, 22 primers were tested and the four most informative ones were selected. The optimisation of the PCR conditions allowed the setting up of a powerful discriminative genotyping method. Moreover, the definition of a quality management method allowed definition of the key steps and the number of replicates to ensure reproducibility of the RAPD pattern. The RAPD was used to study the hot water network of a building. Legionella colonies (91) were isolated from seven locations and genotyped. The diversity of the population in one sample could vary from one to seven different strains. The study of the traceability showed that, in most of the cases, different populations could be present at different locations of the same network.

scholarly journals How Molecular Typing Can Support Legionella Environmental Surveillance in Hot Water Distribution Systems: A Hospital Experience

2020 ◽  
Vol 17 (22) ◽  
pp. 8662
Author(s):  
Luna Girolamini ◽  
Silvano Salaris ◽  
Jessica Lizzadro ◽  
Marta Mazzotta ◽  
Maria Rosaria Pascale ◽  
...  
Keyword(s):  
Legionella Pneumophila ◽  
Molecular Typing ◽  
Distribution Systems ◽  
Water Distribution ◽  
Water Distribution Systems ◽  
Gene Sequencing ◽  
Culture Technique ◽  
Hot Water ◽  
Environmental Surveillance ◽  
Risk Map

In this study, we aimed to associate the molecular typing of Legionella isolates with a culture technique during routine Legionella hospital environmental surveillance in hot water distribution systems (HWDSs) to develop a risk map able to be used to prevent nosocomial infections and formulate appropriate preventive measures. Hot water samples were cultured according to ISO 11731:2017. The isolates were serotyped using an agglutination test and genotyped by sequence-based typing (SBT) for Legionella pneumophila or macrophage infectivity potentiator (mip) gene sequencing for non-pneumophila Legionella species. The isolates’ relationship was phylogenetically analyzed. The Legionella distribution and level of contamination were studied in relation to temperature and disinfectant residues. The culture technique detected 62.21% of Legionella positive samples, characterized by L. pneumophila serogroup 1, Legionella non-pneumophila, or both simultaneously. The SBT assigned two sequence types (STs): ST1, the most prevalent in Italy, and ST104, which had never been isolated before. The mip gene sequencing detected L. anisa and L. rubrilucens. The phylogenetic analysis showed distinct clusters for each species. The distribution of Legionella isolates showed significant differences between buildings, with a negative correlation between the measured level of contamination, disinfectant, and temperature. The Legionella molecular approach introduced in HWDSs environmental surveillance permits (i) a risk map to be outlined that can help formulate appropriate disinfection strategies and (ii) rapid epidemiological investigations to quickly identify the source of Legionella infections.

scholarly journals Molecular Typing of Environmental and Clinical Strains of Vibrio vulnificus Isolated in the Northeastern USA

PLoS ONE ◽  
2013 ◽  
Vol 8 (12) ◽  
pp. e83357 ◽  
Author(s):  
Yann Reynaud ◽  
Steven Pitchford ◽  
Sophie De Decker ◽  
Gary H. Wikfors ◽  
Christopher L. Brown
Keyword(s):  
Molecular Typing ◽  
Vibrio Vulnificus ◽  
Clinical Strains ◽  
Northeastern Usa

scholarly journals Molecular Typing of Legionella pneumophila Isolates in the Province of Quebec from 2005 to 2015

PLoS ONE ◽  
2016 ◽  
Vol 11 (10) ◽  
pp. e0163818 ◽  
Author(s):  
Simon Lévesque ◽  
Cindy Lalancette ◽  
Kathryn Bernard ◽  
Ana Luisa Pacheco ◽  
Réjean Dion ◽  
...  
Keyword(s):  
Legionella Pneumophila ◽  
Molecular Typing

Molecular typing of nosocomial isolates of Legionella pneumophila serogroup 3.

1990 ◽  
Vol 28 (2) ◽  
pp. 242-245 ◽  
Author(s):  
C Tram ◽  
M Simonet ◽  
M H Nicolas ◽  
C Offredo ◽  
F Grimont ◽  
...  
Keyword(s):  
Legionella Pneumophila ◽  
Molecular Typing

scholarly journals Molecular Typing by Pulsed-Field Gel Electrophoresis of Spanish Animal and Human Listeria monocytogenes Isolates

2001 ◽  
Vol 67 (12) ◽  
pp. 5840-5843 ◽  
Author(s):  
A. I. Vela ◽  
J. F. Fernandez-Garayzabal ◽  
J. A. Vazquez ◽  
M. V. Latre ◽  
M. M. Blanco ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Molecular Typing ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Clinical Samples ◽  
Pulsed Field ◽  
Clinical Strains ◽  
Great Genetic Diversity ◽  
Different Sources ◽  
Maize Stalks

ABSTRACT A total of 153 strains of Listeria monocytogenesisolated from different sources (72 from sheep, 12 from cattle, 18 from feedstuffs, and 51 from humans) in Spain from 1989 to 2000 were characterized by pulsed-field gel electrophoresis. The strains ofL. monocytogenes displayed 55 pulsotypes. The 84 animal, 51 human, and 18 feedstuff strains displayed 31, 29, and 7 different pulsotypes, respectively, indicating a great genetic diversity among the Spanish L. monocytogenes isolates studied. L. monocytogenes isolates from clinical samples and feedstuffs consumed by the diseased animals were analyzed in 21 flocks. In most cases, clinical strains from different animals of the same flock had identical pulsotypes, confirming the existence of a listeriosis outbreak. L. monocytogenes strains with pulsotypes identical to those of clinical strains were isolated from silage, potatoes, and maize stalks. This is the first study wherein potatoes and maize stalks are epidemiologically linked with clinical listeriosis.

Sign in / Sign up

Export Citation Format

Share Document