Influence of different particles sizes of anorganic bovine bone matrix on bone repair process: histologic and radiographic analysis of surgically created defects in rabbit calvaria

The objective of this study was to evaluate whether demineralized bovine bone (Gen-ox®) alters bone neoformation in rats submitted to alcoholism. Forty male rats were separated into two groups of 20 rats and distributed as follows: Group E1, which received 25% ethanol and a surgical cavity filled only by a blood clot, and Group E2, which received 25% ethanol and a surgical cavity filled with Gen-ox®. The animals were euthanized at 10, 20, 40 and 60 days after surgery and necropsy was performed. The histomorphological and histometric analyses of the area of connective tissue and bone neoformation showed that the reorganization of the bone marrow and full repair of the surgical cavity in Group E1 occurred more quickly than in Group E2. It was also noted that in the final period the animals in Group E2 showed areas of connective tissue and thick bone trabeculae around the particles of the implant. It can be concluded that the use of Gen-ox® delayed the process of bone repair in alcoholic rats, although it can be used as filling material because it shows osteoconductive activity, as evidenced by bone tissue formation around the graft particles.

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Enhancement of Bone Healing by Local Administration of Carbon Nanotubes Functionalized with Sodium Hyaluronate in Rat Tibiae

Cells Tissues Organs ◽

10.1159/000453030 ◽

2017 ◽

Vol 204 (3-4) ◽

pp. 137-149 ◽

Cited By ~ 2

Author(s):

Vanessa B. Andrade ◽

Marcos A. Sá ◽

Renato M. Mendes ◽

Paulo A. Martins-Júnior ◽

Gerluza A.B. Silva ◽

...

Keyword(s):

Carbon Nanotubes ◽

Bone Repair ◽

Bone Matrix ◽

Sodium Hyaluronate ◽

Repair Process ◽

Bone Defects ◽

Bone Morphogenetic Protein 2 ◽

Morphogenetic Protein ◽

Bone Trabeculae ◽

Endothelial Growth Factor

It has been reported that carbon nanotubes (CNTs) serve as nucleation sites for the deposition of bone matrix and cell proliferation. Here, we evaluated the effects of multi-walled CNTs (MWCNTs) on bone repair of rat tibiae. Furthermore, because sodium hyaluronate (HY) accelerates bone restoration, we associated CNTs with HY (HY-MWCNTs) in an attempt to boost bone repair. The bone defect was created by a 1.6-mm-diameter drill. After 7 and 14 days, tibiae were processed for histological and morphometric analyses. Immunohistochemistry was used to evaluate the expression of vascular endothelial growth factor (VEGF) in bone defects. Expression of osteocalcin (OCN), bone morphogenetic protein-2 (BMP-2), and collagen I (Col I) was assessed by real-time PCR. Histomorphometric analysis showed a similar increase in the percentage of bone trabeculae in tibia bone defects treated with HY and HY-MWCNTs, and both groups presented more organized and thicker bone trabeculae than nontreated defects. Tibiae treated with MWCNTs or HY- MWCNTs showed a higher expression of VEGF. Treatment with MWCNTs or HY-MWCNTs increased the expression of molecules involved in the bone repair process, such as OCN and BMP-2. Also, HY- and MWCNT-treated tibiae had an increased expression of Col I. Thus, it is tempting to conclude that CNTs associated or not with other materials such as HY emerged as a promising biomaterial for bone tissue engineering.

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Bone Repair is Influenced by Different Particle Sizes of Anorganic Bovine Bone Matrix

Journal of Craniofacial Surgery ◽

10.1097/scs.0b013e318286a0a3 ◽

2013 ◽

Vol 24 (4) ◽

pp. 1074-1077 ◽

Cited By ~ 10

Author(s):

Leandro Eduardo Klüppel ◽

Fernando Antonini ◽

Sérgio Olate ◽

Frederico Felipe Nascimento ◽

José Ricardo Albergaria-Barbosa ◽

...

Keyword(s):

Bone Repair ◽

Bone Matrix ◽

Bovine Bone ◽

Particle Sizes

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Native Bovine Hydroxyapatite Powder, Demineralised Bone Matrix Powder, and Purified Bone Collagen Membranes Are Efficient in Repair of Critical-Sized Rat Calvarial Defects

Materials ◽

10.3390/ma13153393 ◽

2020 ◽

Vol 13 (15) ◽

pp. 3393 ◽

Cited By ~ 1

Author(s):

Alexey Veremeev ◽

Roman Bolgarin ◽

Vladimir Nesterenko ◽

Alexander Andreev-Andrievskiy ◽

Anton Kutikhin

Keyword(s):

Bone Mineral ◽

Bone Repair ◽

Bone Matrix ◽

Bone Collagen ◽

Demineralised Bone Matrix ◽

Bovine Bone ◽

Mineral Density ◽

Artificial Defect ◽

Demineralised Bone ◽

Mineral Powder

Here we evaluated the efficacy of bone repair using various native bovine biomaterials (refined hydroxyapatite (HA), demineralised bone matrix (DBM), and purified bone collagen (COLL)) as compared with commercially available bone mineral and bone autografts. We employed a conventional critical-sized (8 mm diameter) rat calvarial defect model (6-month-old male Sprague–Dawley rats, n = 72 in total). The artificial defect was repaired using HA, DBM, COLL, commercially available bone mineral powder, bone calvarial autograft, or remained unfilled (n = 12 animals per group). Rats were euthanised 4 or 12 weeks postimplantation (n = 6 per time point) with the subsequent examination to assess the extent, volume, area, and mineral density of the repaired tissue by means of microcomputed tomography and hematoxylin and eosin staining. Bovine HA and DBM powder exhibited excellent repair capability similar to the autografts and commercially available bone mineral powder while COLL showed higher bone repair rate. We suggest that HA and DBM powder obtained from bovine bone tissue can be equally applied for the repair of bone defects and demonstrate sufficient potential to be implemented into clinical studies.

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Treatment of critical defects produced in calvaria of mice with mesenchymal stem cells

Anais da Academia Brasileira de Ciências ◽

10.1590/s0001-37652012000300026 ◽

2012 ◽

Vol 84 (3) ◽

pp. 841-851 ◽

Cited By ~ 9

Author(s):

Betânia S. Monteiro ◽

Napoleão M. Argôlo-Neto ◽

Nance B. Nardi ◽

Pedro C. Chagastelles ◽

Pablo H. Carvalho ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Bone Repair ◽

Bone Matrix ◽

Cell Types ◽

Repair Process ◽

Treated Group ◽

Control Group ◽

Calvarial Bone

Mesenchymal stem cells (MSC) are present in specialized niches in perivascular regions of adult tissues and are able to differentiate into various cell types, such as those committed to repairing. Bone marrow derived MSC from eight young mice C57BL/ 6 gfp+ were expanded in culture for repairing critical defects in calvarial bone produced in twenty-four young isogenic adult C57BL/6 mice. The animals were subjected to a cranial defect of 6.0mm diameter and divided into two equal experimental groups. Control group did not receive any treatment and the treated group received a MSC pellet containing 1.0 x 10(7) cells/mL into the defects. The group treated with MSC showed increased angiogenesis and amount of new bone deposited on the defect limits than that observed in the control group. The results demonstrated that transplantation of bone marrow-derived MSC of C57BL/6 gfp+ mice to bone critical defects produced in mice calvarial contributes positively to the bone repair process. MSC presets ability to influence the correct functioning of osteoblasts, increases the amount of mobilized cells for the repairing process, speeds up growth, and increases deposition of bone matrix.

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