scholarly journals The action of demineralized bovine bone matrix on bone neoformation in rats submitted to experimental alcoholism

2013 ◽  
Vol 65 (3) ◽  
pp. 715-721 ◽  
Author(s):  
R.L. Buchaim ◽  
J.C. Andreo ◽  
A. C. Rodrigues ◽  
D.V. Buchaim ◽  
D.V. Dias ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Bone Repair ◽  
Blood Clot ◽  
Bone Matrix ◽  
Male Rats ◽  
Bovine Bone ◽  
Filling Material ◽  
Tissue Formation ◽  
Bone Trabeculae ◽  
Bone Tissue Formation

The objective of this study was to evaluate whether demineralized bovine bone (Gen-ox®) alters bone neoformation in rats submitted to alcoholism. Forty male rats were separated into two groups of 20 rats and distributed as follows: Group E1, which received 25% ethanol and a surgical cavity filled only by a blood clot, and Group E2, which received 25% ethanol and a surgical cavity filled with Gen-ox®. The animals were euthanized at 10, 20, 40 and 60 days after surgery and necropsy was performed. The histomorphological and histometric analyses of the area of connective tissue and bone neoformation showed that the reorganization of the bone marrow and full repair of the surgical cavity in Group E1 occurred more quickly than in Group E2. It was also noted that in the final period the animals in Group E2 showed areas of connective tissue and thick bone trabeculae around the particles of the implant. It can be concluded that the use of Gen-ox® delayed the process of bone repair in alcoholic rats, although it can be used as filling material because it shows osteoconductive activity, as evidenced by bone tissue formation around the graft particles.

In Vivo Bone Tissue Formation Induced by Caclium Phosphate Paste Composite with Demineralized Bone Matrix

2007 ◽  
Vol 330-332 ◽  
pp. 1091-1094
Author(s):  
H. Kim ◽  
M. Park ◽  
Su Young Lee ◽  
Kang Yong Lee ◽  
Hyun Min Kim ◽  
...  
Keyword(s):  
Animal Model ◽  
Calcium Phosphate ◽  
Calcium Phosphate Cement ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Marker Gene ◽  
Tissue Formation ◽  
Bone Tissue Formation ◽  
Demineralized Bone

Demineralized bone matrix (DBM)-calcium phosphate cement (CPC) composites were subjected to cellular test of osteogenic potentials and implantation in animal model. The expression of osteogenic marker gene from mouse preosteoblast cell line MC3T3-E1 adhered to the DBM-CPC composite was much higher than plain CPC. In addition, the DBM-CPC composite implanted nude mice revealed osteoinduction between the implanted composite and adjacent tissues, whereas the plain CPC induced osteoconduction.

scholarly journals Comparison of Bone Graft Healing Between Autogenous Bone, Blood Clot and Anorganic Bovine Bone Matrix: Radiographic and Histological Analyses

2013 ◽  
Vol 31 (4) ◽  
pp. 1257-1262
Author(s):  
Sergio Olate ◽  
Henrique Duque Netto ◽  
Leandro E Klüppel ◽  
Bélgica Vásquez ◽  
Maria das Graças A Miranda Chaves ◽  
...  
Keyword(s):  
Bone Graft ◽  
Blood Clot ◽  
Bone Matrix ◽  
Autogenous Bone ◽  
Bovine Bone ◽  
Graft Healing

Enhancement of Bone Healing by Local Administration of Carbon Nanotubes Functionalized with Sodium Hyaluronate in Rat Tibiae

2017 ◽  
Vol 204 (3-4) ◽  
pp. 137-149 ◽  
Author(s):  
Vanessa B. Andrade ◽  
Marcos A. Sá ◽  
Renato M. Mendes ◽  
Paulo A. Martins-Júnior ◽  
Gerluza A.B. Silva ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Bone Repair ◽  
Bone Matrix ◽  
Sodium Hyaluronate ◽  
Repair Process ◽  
Bone Defects ◽  
Bone Morphogenetic Protein 2 ◽  
Morphogenetic Protein ◽  
Bone Trabeculae ◽  
Endothelial Growth Factor

It has been reported that carbon nanotubes (CNTs) serve as nucleation sites for the deposition of bone matrix and cell proliferation. Here, we evaluated the effects of multi-walled CNTs (MWCNTs) on bone repair of rat tibiae. Furthermore, because sodium hyaluronate (HY) accelerates bone restoration, we associated CNTs with HY (HY-MWCNTs) in an attempt to boost bone repair. The bone defect was created by a 1.6-mm-diameter drill. After 7 and 14 days, tibiae were processed for histological and morphometric analyses. Immunohistochemistry was used to evaluate the expression of vascular endothelial growth factor (VEGF) in bone defects. Expression of osteocalcin (OCN), bone morphogenetic protein-2 (BMP-2), and collagen I (Col I) was assessed by real-time PCR. Histomorphometric analysis showed a similar increase in the percentage of bone trabeculae in tibia bone defects treated with HY and HY-MWCNTs, and both groups presented more organized and thicker bone trabeculae than nontreated defects. Tibiae treated with MWCNTs or HY- MWCNTs showed a higher expression of VEGF. Treatment with MWCNTs or HY-MWCNTs increased the expression of molecules involved in the bone repair process, such as OCN and BMP-2. Also, HY- and MWCNT-treated tibiae had an increased expression of Col I. Thus, it is tempting to conclude that CNTs associated or not with other materials such as HY emerged as a promising biomaterial for bone tissue engineering.

scholarly journals Comparison between fractal analysis and radiopacity evaluation as a tool for studying repair of an osseous defect in an animal model using biomaterials

2019 ◽  
Vol 48 (7) ◽  
pp. 20180466
Author(s):  
Nathália R. Gomes ◽  
Juliano D.S. Albergaria ◽  
Jonathas A. de S. Henriques ◽  
Tânia M.P. Amaral ◽  
Evandro N. Abdo ◽  
...  
Keyword(s):  
Animal Model ◽  
Fractal Analysis ◽  
Bone Repair ◽  
Blood Clot ◽  
Bovine Bone ◽  
Final Time ◽  
Significant Difference ◽  
Final Time Point ◽  
Osseous Defect ◽  
Time Point

Objectives: To evaluate bone repair of an osseous defect in a rat animal model through fractal analysis and radiopacity analysis in radiographic images. Methods: 120 rats were subjected to extraction of their first molar and divided into four groups (n = 6/group) according to the material used for bone grafting: mineralized bovine bone, demineralized bovine bone (DBB), blood clot (BC - control) or Bio-Oss® (BO). The animals were sacrificed after 1, 7, 14, 21 and 49 days and subjected to radiographic evaluation. For fractal analysis (FA), a square regionof interest of 30 × 30 pixels was used, and radiopacity was measured as the mean gray scale (MGS) value for three points of 5 × 5 pixels in the apical, medial and coronal regions of the defect. Histomorphometric evaluation was realized as the gold standard for bone neo-formation and maturation of the new osseous matrix. Results: Histomorphometric evaluation suggested that DBB showed faster mineralized deposition and resulted in more mature bone at the final time point of evaluation. Mineralized bovine bone and Bio-Oss presented similar results. The mineralized groups did not show significant differences in bone maturation. The radiopacity analysis revealed a significant difference (p < 0.05) between the DBB and blood clot groups at the final time point. FA did not show any significant differences at the final time point. Conclusions: Mean gray scale seemed to be more effective for the quantification of bone repair than FA in the demineralized group in this animal model. Results for the mineralized groups did not reveal a significant difference, leading to the conclusion that both methods are effective.

Bone tissue formation around two titanium implant surfaces placed in bone defects filled with bone substitute material or blood clot: A pilot study

2019 ◽  
Vol 21 (6) ◽  
pp. 1175-1180
Author(s):  
Guilherme dos Santos Trento ◽  
Rubens Spin‐Neto ◽  
Ana Paula Farnezi Bassi ◽  
Roberta Okamoto ◽  
Marisa Aparecida Cabrini Gabrielli ◽  
...  
Keyword(s):  
Pilot Study ◽  
Bone Tissue ◽  
Bone Substitute ◽  
Blood Clot ◽  
Titanium Implant ◽  
Bone Defects ◽  
Tissue Formation ◽  
Bone Substitute Material ◽  
Substitute Material ◽  
Bone Tissue Formation

Bone Repair is Influenced by Different Particle Sizes of Anorganic Bovine Bone Matrix

2013 ◽  
Vol 24 (4) ◽  
pp. 1074-1077 ◽  
Author(s):  
Leandro Eduardo Klüppel ◽  
Fernando Antonini ◽  
Sérgio Olate ◽  
Frederico Felipe Nascimento ◽  
José Ricardo Albergaria-Barbosa ◽  
...  
Keyword(s):  
Bone Repair ◽  
Bone Matrix ◽  
Bovine Bone ◽  
Particle Sizes

scholarly journals Effect of PDGF-BB, IGF-I Growth Factors and their Combination Carried by Liposomes in Tooth Socket Healing

2013 ◽  
Vol 24 (4) ◽  
pp. 299-307 ◽  
Author(s):  
Fernando Antonio Mauad de Abreu ◽  
Cynthia Lopes Ferreira ◽  
Gerluza Aparecida Borges Silva ◽  
Camila de Oliveira Paulo ◽  
Melissa Nunes Miziara ◽  
...  
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Blood Vessels ◽  
Bone Repair ◽  
Blood Clot ◽  
Healing Process ◽  
Igf I ◽  
Bone Trabeculae ◽  
The Right ◽  
Endothelial Growth Factor

This work evaluated the bone-forming potential of the platelet-derived growth factor isoform BB (PDGF-BB), insulin-like growth factor I (IGF-I), and mixed PDGF-BB/IGF-I delivered in liposomes compared with phosphate buffered saline (PBS), in the healing process of rat tooth sockets. One hundred and twelve Wistar rats were randomized into 7 groups of 16 animals each and were evaluated at 3, 7, 14 and 21 days after extraction of the maxillary second molars. The left sockets were treated with PBS (P), empty liposome (L), IGF-I in PBS (IP), IGF-I in liposome (IL), PDGF-BB in PBS (PDP), PDGF-BB in liposome (PDL) and both growth factors (GFs) together within liposomes (PDIL). The right sockets were filled with blood clot (BC). Histological and histomorphometric analyses were used to evaluate the formation of new bone and blood vessels. Immunohistochemistry was performed to evaluate the expression of osteocalcin and vascular endothelial growth factor (VEGF) during bone repair. Data were tested statistically using a Tukey's test according to a Dunn's analysis and Mann-Whitney U test followed by Kruskal-Wallis one-way analysis. Results were considered significant when p<0.05. A significantly higher percentage of bone trabeculae and a higher number of blood vessels were observed in the IL, PDL and PDIL groups (p<0.05). However, these GF-liposome groups had statistically similar results. Immunohistochemical assays first detected osteocalcin and VEGF expression at 3 days followed by a peak at 7 days. Lower immunoreactivity levels were observed in the BC, L, P, IP and PDP groups compared with the IL, PDL and PDIL groups (p<0.05). The results suggest that GFs carried by liposomes, either in isolated or mixed forms, enhanced the healing process in rat tooth sockets. The differential expression of the osteogenic markers VEGF and osteocalcin in the early phases of bone healing support these findings.

scholarly journals Native Bovine Hydroxyapatite Powder, Demineralised Bone Matrix Powder, and Purified Bone Collagen Membranes Are Efficient in Repair of Critical-Sized Rat Calvarial Defects

Materials ◽  
2020 ◽  
Vol 13 (15) ◽  
pp. 3393 ◽  
Author(s):  
Alexey Veremeev ◽  
Roman Bolgarin ◽  
Vladimir Nesterenko ◽  
Alexander Andreev-Andrievskiy ◽  
Anton Kutikhin
Keyword(s):  
Bone Mineral ◽  
Bone Repair ◽  
Bone Matrix ◽  
Bone Collagen ◽  
Demineralised Bone Matrix ◽  
Bovine Bone ◽  
Mineral Density ◽  
Artificial Defect ◽  
Demineralised Bone ◽  
Mineral Powder

Here we evaluated the efficacy of bone repair using various native bovine biomaterials (refined hydroxyapatite (HA), demineralised bone matrix (DBM), and purified bone collagen (COLL)) as compared with commercially available bone mineral and bone autografts. We employed a conventional critical-sized (8 mm diameter) rat calvarial defect model (6-month-old male Sprague–Dawley rats, n = 72 in total). The artificial defect was repaired using HA, DBM, COLL, commercially available bone mineral powder, bone calvarial autograft, or remained unfilled (n = 12 animals per group). Rats were euthanised 4 or 12 weeks postimplantation (n = 6 per time point) with the subsequent examination to assess the extent, volume, area, and mineral density of the repaired tissue by means of microcomputed tomography and hematoxylin and eosin staining. Bovine HA and DBM powder exhibited excellent repair capability similar to the autografts and commercially available bone mineral powder while COLL showed higher bone repair rate. We suggest that HA and DBM powder obtained from bovine bone tissue can be equally applied for the repair of bone defects and demonstrate sufficient potential to be implemented into clinical studies.

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