Native Bovine Hydroxyapatite Powder, Demineralised Bone Matrix Powder, and Purified Bone Collagen Membranes Are Efficient in Repair of Critical-Sized Rat Calvarial Defects

Here we evaluated the efficacy of bone repair using various native bovine biomaterials (refined hydroxyapatite (HA), demineralised bone matrix (DBM), and purified bone collagen (COLL)) as compared with commercially available bone mineral and bone autografts. We employed a conventional critical-sized (8 mm diameter) rat calvarial defect model (6-month-old male Sprague–Dawley rats, n = 72 in total). The artificial defect was repaired using HA, DBM, COLL, commercially available bone mineral powder, bone calvarial autograft, or remained unfilled (n = 12 animals per group). Rats were euthanised 4 or 12 weeks postimplantation (n = 6 per time point) with the subsequent examination to assess the extent, volume, area, and mineral density of the repaired tissue by means of microcomputed tomography and hematoxylin and eosin staining. Bovine HA and DBM powder exhibited excellent repair capability similar to the autografts and commercially available bone mineral powder while COLL showed higher bone repair rate. We suggest that HA and DBM powder obtained from bovine bone tissue can be equally applied for the repair of bone defects and demonstrate sufficient potential to be implemented into clinical studies.

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Heterologous demineralised bone matrix is efficient for the repair of critical-sized rat calvarial defects

Fundamental and Clinical Medicine ◽

10.23946/2500-0764-2020-5-3-24-34 ◽

2020 ◽

Vol 5 (3) ◽

pp. 24-34

Author(s):

A. V. Veremeev ◽

R. N. Bolgarin ◽

V. G. Nesterenko ◽

A. A. Andreev-Andrievskiy

Keyword(s):

Bone Repair ◽

Bone Matrix ◽

Microcomputed Tomography ◽

Demineralised Bone Matrix ◽

Calvarial Defect ◽

Sprague Dawley ◽

Mineral Density ◽

Calvarial Defects ◽

Demineralised Bone ◽

Bone Autograft

Aim. To evaluate the efficacy of heterologous demineralised bone matrix (DBM) for the replacement of bone defects using a critical-sized rat calvarial defect model. Materials and Methods. For the experiments, we used 48 Sprague-Dawley rats (4.5 to 6 months of age). Critical-sized (8 mm diameter) calvarial defect was filled by the bone autograft, heterologous DBM, or comparator product (Geistlich BioOss®) or remained unfilled (negative control). Upon 4 or 12 weeks, rats were euthanised with the subsequent investigation of the defect and adjacent tissues by means of hematoxylin and eosin staining (mineralized tissue area to the defect area ratio) and microcomputed tomography (volume, thickness, and mineral density of the repaired tissue). Results. In our experimental setting, bone autograft was the most efficient in bone repair. Heterologous DBM and comparator product were equally efficient in filling the defect and did not show any statistically significant differences regarding any of the parameters. Microcomputed tomography and routine histological examination demonstrated concordant results. Conclusion. Heterologous DBM is efficient for the repair of critical-sized rat calvarial defects.

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Аpplication of xenogeneic native bone collagen for bone repair in critical-sized rat calvarial defect model

Fundamental and Clinical Medicine ◽

10.23946/2500-0764-2020-5-2-8-21 ◽

2020 ◽

Vol 5 (2) ◽

pp. 8-21

Author(s):

A. V. Veremeev ◽

R. N. Bolgarin ◽

V. G. Nesterenko ◽

A. A. Andreev-Andrievskiy

Keyword(s):

Bone Mineral ◽

Bone Repair ◽

Bone Collagen ◽

Calvarial Defect ◽

Mineral Density ◽

Defect Model ◽

Artificial Defect ◽

Regeneration Rate ◽

Native Bone ◽

Rat Calvarial Defect

Aim. To evaluate the efficacy of bone repair using xenogeneic native bone collagen (Bongraf COLLAGEN) as compared to other widely applied orthopaedic solutions (xenogeneic native bone mineral Geistlich Bio-Oss® and bone autograft).Materials and Methods. We employed a conventional critical-sized (8 mm) rat calvarial defect model (48 Wistar or Sprague-Dawley rats). The artificial defect was repaired using Bongraf COLLAGEN, Geistlich Bio-Oss® utilised as a comparator, bone calvarial autograft, or remained unfilled (n = 6 per group). Rats were euthanised 4 or 12 weeks postimplantation (n = 3 per time point) with the subsequent examination (repair extent, volume, thickness and mineral density of the repaired tissue) by means of microcomputed tomography and hematoxylin and eosin staining.Results. Expectedly, highest volume, thickness and mineral density of the repaired tissue have been observed in defects filled with autografts. Bongraf COLLAGEN and Geistlich Bio-Oss® also demonstrated a comparable and significant repair capability, yet the former option demonstrated higher bone regeneration rate.Conclusion. Xenogeneic native bone collagen (Bongraf COLLAGEN) is comparable with xenogeneic native bone mineral (Geistlich Bio-Oss®).

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The action of demineralized bovine bone matrix on bone neoformation in rats submitted to experimental alcoholism

Arquivo Brasileiro de Medicina Veterinária e Zootecnia ◽

10.1590/s0102-09352013000300016 ◽

2013 ◽

Vol 65 (3) ◽

pp. 715-721 ◽

Cited By ~ 1

Author(s):

R.L. Buchaim ◽

J.C. Andreo ◽

A. C. Rodrigues ◽

D.V. Buchaim ◽

D.V. Dias ◽

...

Keyword(s):

Connective Tissue ◽

Bone Repair ◽

Blood Clot ◽

Bone Matrix ◽

Male Rats ◽

Bovine Bone ◽

Filling Material ◽

Tissue Formation ◽

Bone Trabeculae ◽

Bone Tissue Formation

The objective of this study was to evaluate whether demineralized bovine bone (Gen-ox®) alters bone neoformation in rats submitted to alcoholism. Forty male rats were separated into two groups of 20 rats and distributed as follows: Group E1, which received 25% ethanol and a surgical cavity filled only by a blood clot, and Group E2, which received 25% ethanol and a surgical cavity filled with Gen-ox®. The animals were euthanized at 10, 20, 40 and 60 days after surgery and necropsy was performed. The histomorphological and histometric analyses of the area of connective tissue and bone neoformation showed that the reorganization of the bone marrow and full repair of the surgical cavity in Group E1 occurred more quickly than in Group E2. It was also noted that in the final period the animals in Group E2 showed areas of connective tissue and thick bone trabeculae around the particles of the implant. It can be concluded that the use of Gen-ox® delayed the process of bone repair in alcoholic rats, although it can be used as filling material because it shows osteoconductive activity, as evidenced by bone tissue formation around the graft particles.

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Bone quality associated with daily intake of coffee: a biochemical, radiographic and histometric study

Brazilian Dental Journal ◽

10.1590/s0103-64402010000300004 ◽

2010 ◽

Vol 21 (3) ◽

pp. 199-204 ◽

Cited By ~ 16

Author(s):

Suzie Aparecida Lacerda ◽

Renata Inahara Matuoka ◽

Rander Moreira Macedo ◽

Sergio Olavo Petenusci ◽

Alessandra Aparecida Campos ◽

...

Keyword(s):

Bone Mineral Density ◽

Bone Quality ◽

Bone Mineral ◽

Bone Repair ◽

Daily Intake ◽

Male Rats ◽

Lower Amount ◽

Caffeine Intake ◽

Mineral Density ◽

Coffee Intake

Caffeine induces loss of calcium and influences the normal development of bone. This study investigated the effects of coffee on bone metabolism in rats by biochemical measurement of calcium, bone densitometry and histometry. Male rats, born of female treated daily with coffee and with coffee intake since born, were anesthetized, subjected to extraction of the upper right incisor, and sacrificed 7, 21 and 42 days after surgery. Blood and urine samples were taken, and their maxilla radiographed and processed to obtain 5-µm-thick semi-serial sections stained with hematoxylin and eosin. The volume and bone quality were estimated using an image-analysis software. The results showed significantly greater amount of calcium in the plasma (9.40 ± 1.73 versus 9.80 ± 2.05 mg%) and urine (1.00 ± 0.50 versus 1.25 ± 0.70 mg/24 h) and significantly less amount in bone (90.0 ± 1.94 versus 86.0 ± 2.12 mg/mg bone), reduced bone mineral density (1.05 ± 0.11 versus 0.65 ± 0.15 mmAL), and lower amount of bone (76.19 ± 1.6 versus 53.41 ± 2.1 %) (ANOVA; p≤0.01) in animals treated with coffee sacrificed after 42 days. It may be concluded that coffee/caffeine intake caused serious adverse effects on calcium metabolism in rats, including increased levels of calcium in the urine and plasma, decreased bone mineral density and lower volume of bone, thus delaying the bone repair process.

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